Cellular localization of gonadotropin-releasing hormone (GnRH) I and GnRH II in first-trimester human placenta and decidua

Chun Shan Chou, Alexander G. Beristain, Colin D. MacCalman, Peter C K Leung

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

There is increasing evidence to suggest that the classical form of GnRH (GnRH I) and the second mammalian form of this hormone, GnRH II, play regulatory roles in human implantation and placentation. To date, the cellular distribution of these two hormones at the maternal-fetal interface remains poorly characterized. In these studies, we localized GnRH I and GnRH II expression in human placenta and decidua to distinct subpopulations of cells isolated from these tissues and in a chorionic villous/decidual tissue coculture system that mimics many of the cellular events of early placentation. GnRH I and GnRH II mRNA transcripts were detected in first-trimester placenta, whereas only GnRH I was detected in tissues obtained at term. Both hormones were further immunolocalized to the mononucleate villous and distinct subpopulations of extravillous cytotrophoblasts of the placenta in vivo and in vitro. In contrast, GnRH I but not GnRH II was expressed in the outer multinucleated syncytial trophoblast layer of first trimester chorionic villi and in cultures of villous cytotrophoblasts allowed to undergo differentiation and fusion in vitro. GnRH I and GnRH II were also found to be coexpressed in first-trimester decidua and primary cultures of decidual stromal cells. Collectively, these observations demonstrate that GnRH I and GnRH II have both common and discrete cellular distributions in the placenta and decidua and suggest that these two hormones are capable of eliciting their biological actions in an autocrine and/or paracrine manner within and between these maternal and fetal cellular compartments.

Original languageEnglish
Pages (from-to)1459-1466
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume89
Issue number3
DOIs
Publication statusPublished - Mar 2004

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Decidua
First Pregnancy Trimester
Gonadotropin-Releasing Hormone
Placenta
Trophoblasts
Hormones
Placentation
Tissue
Mothers
Chorionic Villi
Stromal Cells
Coculture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Cellular localization of gonadotropin-releasing hormone (GnRH) I and GnRH II in first-trimester human placenta and decidua. / Chou, Chun Shan; Beristain, Alexander G.; MacCalman, Colin D.; Leung, Peter C K.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 89, No. 3, 03.2004, p. 1459-1466.

Research output: Contribution to journalArticle

Chou, Chun Shan ; Beristain, Alexander G. ; MacCalman, Colin D. ; Leung, Peter C K. / Cellular localization of gonadotropin-releasing hormone (GnRH) I and GnRH II in first-trimester human placenta and decidua. In: Journal of Clinical Endocrinology and Metabolism. 2004 ; Vol. 89, No. 3. pp. 1459-1466.
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