Cellular interaction of integrin α3β1 with laminin 5 promotes gap junctional communication

Paul D. Lampe, Beth P. Nguyen, Susana Gil, Marcia Usui, John Olerud, Yoshikazu Takada, William G. Carter

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Wounding of skin activates epidermal cell migration over exposed dermal collagen and fibronectin and over laminin 5 secreted into the provisional basement membrane. Gap junctional intercellular communication (GJIC) has been proposed to integrate the individual motile cells into a synchronized colony. We found that outgrowths of human keratinocytes in wounds or epibole cultures display parallel changes in the expression of laminin 5, integrin α3β1, E- cadherin, and the gap junctional protein connexin 43. Adhesion of keratinocytes on laminin 5, collagen, and fibronectin was found to differentially regulate GJIC. When keratinocytes were adhered on laminin 5, both structural (assembly of connexin 43 in gap junctions) and functional (dye transfer) assays showed a two- to threefold increase compared with collagen and five- to eightfold over fibronectin. Based on studies with immobilized integrin antibody and integrin-transfected Chinese hamster ovary cells, the interaction of integrin α3β1 with laminin 5 was sufficient to promote GJIC. Mapping of intermediate steps in the pathway linking α3β1- laminin 5 interactions to GJIC indicated that protein trafficking and Rho signaling were both required. We suggest that adhesion of epithelial cells to laminin 5 in the basement membrane via α3β1 promotes GJIC that integrates individual cells into synchronized epiboles.

Original languageEnglish
Pages (from-to)1735-1747
Number of pages13
JournalJournal of Cell Biology
Volume143
Issue number6
DOIs
Publication statusPublished - Dec 14 1998
Externally publishedYes

Fingerprint

Integrins
Communication
Keratinocytes
Fibronectins
Connexin 43
Collagen
Basement Membrane
Immobilized Antibodies
Skin
Gap Junctions
Protein Transport
Cadherins
Cricetulus
kalinin
Cell Communication
Cell Movement
Ovary
Coloring Agents
Epithelial Cells
Wounds and Injuries

Keywords

  • Epidermis
  • Gap junctions
  • Integrins
  • Intracellular protein trafficking
  • Laminin 5

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lampe, P. D., Nguyen, B. P., Gil, S., Usui, M., Olerud, J., Takada, Y., & Carter, W. G. (1998). Cellular interaction of integrin α3β1 with laminin 5 promotes gap junctional communication. Journal of Cell Biology, 143(6), 1735-1747. https://doi.org/10.1083/jcb.143.6.1735

Cellular interaction of integrin α3β1 with laminin 5 promotes gap junctional communication. / Lampe, Paul D.; Nguyen, Beth P.; Gil, Susana; Usui, Marcia; Olerud, John; Takada, Yoshikazu; Carter, William G.

In: Journal of Cell Biology, Vol. 143, No. 6, 14.12.1998, p. 1735-1747.

Research output: Contribution to journalArticle

Lampe, PD, Nguyen, BP, Gil, S, Usui, M, Olerud, J, Takada, Y & Carter, WG 1998, 'Cellular interaction of integrin α3β1 with laminin 5 promotes gap junctional communication', Journal of Cell Biology, vol. 143, no. 6, pp. 1735-1747. https://doi.org/10.1083/jcb.143.6.1735
Lampe, Paul D. ; Nguyen, Beth P. ; Gil, Susana ; Usui, Marcia ; Olerud, John ; Takada, Yoshikazu ; Carter, William G. / Cellular interaction of integrin α3β1 with laminin 5 promotes gap junctional communication. In: Journal of Cell Biology. 1998 ; Vol. 143, No. 6. pp. 1735-1747.
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