Cell-mediated immune response to human immunodeficiency virus (hiv) type 1 in seronegative homosexual men with recent sexual exposure to hiv-1

Mario Clerici, Janis V. Giorgi, Chen Cheng Chou, Vaheideh K. Gudeman, Jerome A. Zack, Phalguni Gupta, Hong Nerng Ho, Parunag G. Nishanian, Jay A. Berzofsky, Gene M. Shearer

Research output: Contribution to journalArticle

330 Citations (Scopus)

Abstract

Although human immunodeficiency virus (HIV) type 1 infection is efficiently transmitted by sexual intercourse, some individuals whose sexual behavior places them at extremely high risk for infection have nevertheless remained HIV-1-seronegative. An investigation was undertaken to determine whether such individuals have circulating T helper cells that are sensitized to HIV- 1. Five very high risk men who had recent sexual exposure to HIV-1 were studied. Peripheral blood mononuclear cells from all 5 produced interleukin (IL)-2 in culture in response to synthetic amphipathic HIV-1 envelope peptides. One of the 5 high-risk men has subsequently serocon- verted, while 4 have remained seronegative. All were initially culture-negative, and those who have remained seronegative were also virus-negative by polymerase chain reaction (PCR) testing 10 months after they were first studied. These results demonstrate that a cell-mediated immune response to HIV-1 can be detected in the absence of a humoral immune response in individuals recently exposed to HIV-1. Furthermore, IL-2 production by T cells in response to synthetic peptides may be a more sensitive test for exposure to HIV-1 than antibody, lymphoproliferation, or PCR tests.

Original languageEnglish
Pages (from-to)1012-1019
Number of pages8
JournalJournal of Infectious Diseases
Volume165
Issue number6
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

Fingerprint

Molecular Sequence Data
HIV Antigens
Mononuclear Leukocytes
Los Angeles
Oligonucleotide Probes
Homosexuality
Viral DNA
Sexually Transmitted Diseases
Helper-Inducer T-Lymphocytes
Cellular Immunity
HIV Infections
Interleukin-2
HIV-1
Amino Acid Sequence
Cohort Studies
Polymerase Chain Reaction
Peptides
Sexual Minorities
Coitus
Virus Diseases

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Cell-mediated immune response to human immunodeficiency virus (hiv) type 1 in seronegative homosexual men with recent sexual exposure to hiv-1. / Clerici, Mario; Giorgi, Janis V.; Chou, Chen Cheng; Gudeman, Vaheideh K.; Zack, Jerome A.; Gupta, Phalguni; Ho, Hong Nerng; Nishanian, Parunag G.; Berzofsky, Jay A.; Shearer, Gene M.

In: Journal of Infectious Diseases, Vol. 165, No. 6, 01.01.1992, p. 1012-1019.

Research output: Contribution to journalArticle

Clerici, M, Giorgi, JV, Chou, CC, Gudeman, VK, Zack, JA, Gupta, P, Ho, HN, Nishanian, PG, Berzofsky, JA & Shearer, GM 1992, 'Cell-mediated immune response to human immunodeficiency virus (hiv) type 1 in seronegative homosexual men with recent sexual exposure to hiv-1', Journal of Infectious Diseases, vol. 165, no. 6, pp. 1012-1019. https://doi.org/10.1093/infdis/165.6.1012
Clerici, Mario ; Giorgi, Janis V. ; Chou, Chen Cheng ; Gudeman, Vaheideh K. ; Zack, Jerome A. ; Gupta, Phalguni ; Ho, Hong Nerng ; Nishanian, Parunag G. ; Berzofsky, Jay A. ; Shearer, Gene M. / Cell-mediated immune response to human immunodeficiency virus (hiv) type 1 in seronegative homosexual men with recent sexual exposure to hiv-1. In: Journal of Infectious Diseases. 1992 ; Vol. 165, No. 6. pp. 1012-1019.
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