Cell coupling regulates Ins1, Pdx-1 and MafA to promote insulin secretion in mouse pancreatic beta cells

Kai Chiang Yang, Zhi Qi, Goichi Yanai, Yasumasa Shirouza, Dai Hua Lu, Hsuan Shu Lee, Shoichiro Sumi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The intracellular contact between pancreatic β-cells plays an important role in functional insulin secretion. We propose cell coupling may regulate glucose-stimulated insulin gene expression. MIN-6, a murine β-cell line, was cultured on the Lipidure-coat dish for cell clusters formation. The responses to glucose-stimulation between monolayers and clusters were compared. The coupling of β-cells was evaluated by immunohistochemical (IHC) staining. After the establishment of cell coupling was confirmed, the transcription factors of insulin gene expression were assessed by RT-PCR. Results showed that in addition to secrete insulin in a glucose-regulated manner, MIN-6 clusters had biphasic insulin secretion. Relative to monolayer, clusters have higher stimulation-index and lower constitutive insulin release. IHC staining showed the connexin 36 highly expressed in clusters. Compared with monolayers, the mRNA expression of insulin 1 (Ins1) and homeodomain transcription factor pancreatic/duodenal homebox-1 (Pdx-1) of clusters were downregulated in low glucose level. On the contrary, these gene expressions were upregulated when clusters cultured in high glucose environment. The V-maf musculoaponeurotic fibrosarcoma oncogene homologue A (MafA) was relative highly expressed in clusters under high glucose condition. This study reveals that cell coupling regulates glucose-stimulated insulin gene expression to promote insulin secretion in mouse β-cells.

Original languageEnglish
Pages (from-to)1853-1860
Number of pages8
JournalProcess Biochemistry
Volume46
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Insulin
Insulin-Secreting Cells
Glucose
Gene expression
Gene Expression
Monolayers
Transcription factors
Transcription Factors
Biphasic Insulins
Staining and Labeling
Glycocalyx
Fibrosarcoma
Oncogenes
Down-Regulation
Cells
Cell Line
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Beta-cell
  • Cell coupling
  • Glucose-stimulated insulin secretion

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Cell coupling regulates Ins1, Pdx-1 and MafA to promote insulin secretion in mouse pancreatic beta cells. / Yang, Kai Chiang; Qi, Zhi; Yanai, Goichi; Shirouza, Yasumasa; Lu, Dai Hua; Lee, Hsuan Shu; Sumi, Shoichiro.

In: Process Biochemistry, Vol. 46, No. 9, 09.2011, p. 1853-1860.

Research output: Contribution to journalArticle

Yang, Kai Chiang ; Qi, Zhi ; Yanai, Goichi ; Shirouza, Yasumasa ; Lu, Dai Hua ; Lee, Hsuan Shu ; Sumi, Shoichiro. / Cell coupling regulates Ins1, Pdx-1 and MafA to promote insulin secretion in mouse pancreatic beta cells. In: Process Biochemistry. 2011 ; Vol. 46, No. 9. pp. 1853-1860.
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