Cell-based semiquantitative assay for sulfated glycosaminoglycans facilitating the identification of chondrogenesis

Ching Yu Yen, Yu Wei Wu, Chao Nan Hsiung, Min I. Yeh, Yi Ming Lin, Sheng Yang Lee

Research output: Contribution to journalArticle

Abstract

Glycosaminoglycans (GAGs), in particular chondroitin sulfate, are an accepted marker of chondrogenic cells. In this study, a cell-based sulfated GAG assay for identifying the chondrogenesis of mesenchymal stem cells was developed. Based on fluorescent staining using safranin O and 4',6-diamidino-2-phenylindole (DAPI), this method was highly sensitive. The results were both qualitative and quantitative. The method is suitable for identifying the chondrogenic process and also for screening compounds. The method may be helpful for discovering novel bioactive compounds for cartilage regeneration.

Original languageEnglish
Pages (from-to)41-43
Number of pages3
JournalAnalytical Biochemistry
Volume486
DOIs
Publication statusPublished - Jul 21 2015

Keywords

  • Chondrogenesis
  • DAPI
  • Glycosaminoglycan
  • Safranin O
  • Screening

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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