Cell adhesion regulates the plasminogen activator inhibitor-1 gene expression in anchorage-dependent cells

Chun Chung Lee, Kou Gi Shyu, Shankung Lin, Bao Wei Wang, Ya Chen Liu, Hang Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Plasminogen activator inhibitor-1 (PAI-1) is the primary inhibitor of both tissue- and urokinase-type plasminogen activators (t-PA, u-PA). PAI-1 also regulates the attachment of cells to the adhesive glycoprotein vitronectin (VN). PAI-1 gene expression has been observed in various cell types, and many regulatory factors have been identified to play a role in PAI-1 gene transcription. The complete picture of how the PAI-1 gene is expressed when cells adhere to a culture plate has not been fully elucidated. We found that in anchorage-dependent cells, PAI-1 gene was up-regulated when cells were beginning to attach to a culture dish and was down-regulated when cells had attached completely. The PAI-1 gene expression was induced only in adhered cells but not in non-adhered cells. The regulation of PAI-1 protein was also found in both culture medium and cell lysate when cells were attached to a culture dish. Our experiment indicates that vitronectin and fibronectin, as components of ECM, may be the factors involved in the regulation of PAI-1 gene expression. PAI-1, as an inhibitor of the interaction between vitronectin and integrin αvβ3, may also be a regulator of its own expression.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number1
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • αvβ3
  • Adhesion
  • ECM
  • Fibronectin
  • Integrin
  • PAI-1
  • Plasminogen
  • t-PA
  • uPA
  • Vitronectin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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