Cell adhesion regulates the plasminogen activator inhibitor-1 gene expression in anchorage-dependent cells

Chun Chung Lee, Kou Gi Shyu, Shankung Lin, Bao Wei Wang, Ya Chen Liu, Hang Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Plasminogen activator inhibitor-1 (PAI-1) is the primary inhibitor of both tissue- and urokinase-type plasminogen activators (t-PA, u-PA). PAI-1 also regulates the attachment of cells to the adhesive glycoprotein vitronectin (VN). PAI-1 gene expression has been observed in various cell types, and many regulatory factors have been identified to play a role in PAI-1 gene transcription. The complete picture of how the PAI-1 gene is expressed when cells adhere to a culture plate has not been fully elucidated. We found that in anchorage-dependent cells, PAI-1 gene was up-regulated when cells were beginning to attach to a culture dish and was down-regulated when cells had attached completely. The PAI-1 gene expression was induced only in adhered cells but not in non-adhered cells. The regulation of PAI-1 protein was also found in both culture medium and cell lysate when cells were attached to a culture dish. Our experiment indicates that vitronectin and fibronectin, as components of ECM, may be the factors involved in the regulation of PAI-1 gene expression. PAI-1, as an inhibitor of the interaction between vitronectin and integrin αvβ3, may also be a regulator of its own expression.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number1
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Plasminogen Activator Inhibitor 1
Cell adhesion
Gene expression
Cell Adhesion
Gene Expression
Vitronectin
Genes
Replication Protein C
Military electronic countermeasures
Urokinase-Type Plasminogen Activator
Tissue Plasminogen Activator
Transcription
Fibronectins
Cell culture
Integrins
Adhesives
Culture Media
Glycoproteins

Keywords

  • αvβ3
  • Adhesion
  • ECM
  • Fibronectin
  • Integrin
  • PAI-1
  • Plasminogen
  • t-PA
  • uPA
  • Vitronectin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cell adhesion regulates the plasminogen activator inhibitor-1 gene expression in anchorage-dependent cells. / Lee, Chun Chung; Shyu, Kou Gi; Lin, Shankung; Wang, Bao Wei; Liu, Ya Chen; Chang, Hang.

In: Biochemical and Biophysical Research Communications, Vol. 291, No. 1, 2002, p. 185-190.

Research output: Contribution to journalArticle

Lee, Chun Chung ; Shyu, Kou Gi ; Lin, Shankung ; Wang, Bao Wei ; Liu, Ya Chen ; Chang, Hang. / Cell adhesion regulates the plasminogen activator inhibitor-1 gene expression in anchorage-dependent cells. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 291, No. 1. pp. 185-190.
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