cDNA Cloning and characterization of a plant protein that may be associated with the harpin(PSS)-mediated hypersensitive response

Cheng-Hsien Chen, Hao Jan Lin, Mang Jye Ger, David T. Chow, Teng Yung Feng

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Hypersensitive response-assisting protein (HRAP) is a novel plant protein that can intensify the harpin(PSS)-mediated hypersensitive response (HR) in harpin(PSS)-insensitive plants, such as the vegetative stage of sweet pepper. In this report, we identified a HRAP cDNA clone from sweet pepper (Capsicum annuum cv. ECW). The sequence of this cDNA clone showed no appreciable similarity to any other known sequences. However, it contained three positively charged regions, a typical signal peptide and a cAMP-dependent phosphorylation site. The hrap mRNA accumulated preferentially during the incompatible interaction of sweet pepper leaves with a pathogenic bacterium, Pseudomonas syringae pv. syringae. When the hrap gene transcription level was high, the sweet pepper leaves readily expressed the harpin(PSS)-mediated HR. The hrap gene transcription level in sweet pepper was also higher during the reproductive stage than during the vegetative stage. The HRAP distribution in an individual plant and different plant species was investigated. We found that all the organs of sweet pepper, except fruit, could express two different forms of HRAP. Moreover, the hrap gene was presented in many plant species including tobacco, Arabidopsis, and rice. In conclusion, our results suggest that the hrap gene is widely distributed throughout the plant world and its transcription level correlates with plant sensitivity to harpin(PSS). The interaction between HRAP and harpin(PSS) reveals a novel way to interpret the interaction mechanism between plants and bacterial pathogens.

Original languageEnglish
Pages (from-to)429-438
Number of pages10
JournalPlant Molecular Biology
Volume43
Issue number4
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Capsicum
Plant Proteins
Cloning
hypersensitive response
plant proteins
sweet peppers
Organism Cloning
molecular cloning
Complementary DNA
Transcription
Genes
Proteins
transcription (genetics)
proteins
vegetative growth
Phosphorylation
Tobacco
genes
Clone Cells
Pathogens

Keywords

  • Defense-related protein
  • Harpin(PSS)
  • Hypersensitive response
  • Hypersensitive response-assisting protein
  • Sweet pepper

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

cDNA Cloning and characterization of a plant protein that may be associated with the harpin(PSS)-mediated hypersensitive response. / Chen, Cheng-Hsien; Lin, Hao Jan; Ger, Mang Jye; Chow, David T.; Feng, Teng Yung.

In: Plant Molecular Biology, Vol. 43, No. 4, 2000, p. 429-438.

Research output: Contribution to journalArticle

Chen, Cheng-Hsien ; Lin, Hao Jan ; Ger, Mang Jye ; Chow, David T. ; Feng, Teng Yung. / cDNA Cloning and characterization of a plant protein that may be associated with the harpin(PSS)-mediated hypersensitive response. In: Plant Molecular Biology. 2000 ; Vol. 43, No. 4. pp. 429-438.
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