CD44 is a physiological E-selectin ligand on neutrophils

Yoshio Katayama, Andrés Hidalgo, Jungshan Chang, Anna Peired, Paul S. Frenette

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

The selectin family of adhesion molecules and their glycoconjugated ligands are essential for blood polymorphonuclear neutrophil (PMN) extravasation into inflammatory and infectious sites. However, E-selectin ligands on PMNs are not well characterized. We show here that CD44 immunopurified from G-CSF-differentiated 32D cells or from peripheral blood PMNs binds specifically to E-selectin. In contrast, CD44 extracted from bone marrow stromal or brain endothelial cell lines does not interact with E-selectin, suggesting cell-specific posttranslational modifications of CD44. PMN-derived CD44 binding activity is mediated by sialylated, α(1,3) fucosylated, N-linked glycans. CD44 enables slow leukocyte rolling on E-selectin expressed on inflamed endothelium in vivo and cooperates with P-selectin glycoprotein ligand-1 to recruit neutrophils into thioglycollate-induced peritonitis and staphylococcal enterotoxin A-injected skin pouch. CD44 extracted from human PMNs also binds to E-selectin. Moreover, we demonstrate that CD44 is hypofucosylated in PMNs from a patient with leukocyte adhesion deficiency type II, suggesting that it contributes to the syndrome. These findings thus suggest broader roles for CD44 in the innate immune response and uncover a potential new target for diseases in which selectins play a prominent role.

Original languageEnglish
Pages (from-to)1183-1189
Number of pages7
JournalJournal of Experimental Medicine
Volume201
Issue number8
DOIs
Publication statusPublished - Apr 18 2005
Externally publishedYes

Fingerprint

E-Selectin
Neutrophils
Ligands
Selectins
Leukocyte Rolling
Thioglycolates
Granulocyte Colony-Stimulating Factor
Post Translational Protein Processing
Peritonitis
Innate Immunity
Endothelium
Polysaccharides
Endothelial Cells
Bone Marrow
Cell Line
Skin
Brain

ASJC Scopus subject areas

  • Immunology

Cite this

CD44 is a physiological E-selectin ligand on neutrophils. / Katayama, Yoshio; Hidalgo, Andrés; Chang, Jungshan; Peired, Anna; Frenette, Paul S.

In: Journal of Experimental Medicine, Vol. 201, No. 8, 18.04.2005, p. 1183-1189.

Research output: Contribution to journalArticle

Katayama, Y, Hidalgo, A, Chang, J, Peired, A & Frenette, PS 2005, 'CD44 is a physiological E-selectin ligand on neutrophils', Journal of Experimental Medicine, vol. 201, no. 8, pp. 1183-1189. https://doi.org/10.1084/jem.20042014
Katayama, Yoshio ; Hidalgo, Andrés ; Chang, Jungshan ; Peired, Anna ; Frenette, Paul S. / CD44 is a physiological E-selectin ligand on neutrophils. In: Journal of Experimental Medicine. 2005 ; Vol. 201, No. 8. pp. 1183-1189.
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