CD24 expression indicates healthier phenotype and less tendency of cellular senescence in human nucleus pulposus cells

Shu Hua Yang, Ming Hsiao Hu, Chang Chin Wu, Chih Wei Chen, Yuan Hui Sun, Kai Chiang Yang

Research output: Contribution to journalArticle

Abstract

Identification of specific cell markers is crucial for recognizing functionally healthy nucleus pulposus (NP) cells. The objective of this study was to investigate the role of CD24 expression in adult human NP cells. Cells were retrieved from NP tissues of 20 patients (aged 17–44) operated on for lumbar disc herniation. Based on CD24 expression, NP cells were separated by sorting and then used to examine phenotypic behavior, the effects of culture conditions and cellular senescence pathway related proteins. CD24 expression was positive in 35.5 ± 3.7% (range 9.1–65.2%) of NP cells. Consistently, normoxic expansion and serial passages in monolayers decreased percentage positivity for CD24 in NP cells. CD24 NP cells showed a markedly decreased GSK-3β activity and increased mitogen-activated protein kinase phosphorylation accompanying by an increased β-catenin expression. Higher levels of matrix metalloproteinases, as well as lower levels of ACAN and COL2 in CD24 cells, indicated the breakdown and reduced the formation of key extracellular matrix components. CD24+ NP cells presented a more favorable phenotype while CD24 cells showed a more prominent cellular senescence fate. CD24 in NP cells may be a surrogate marker of healthy cells, in the cell-based therapeutic treatment of degenerative disc disorders.

Original languageEnglish
Pages (from-to)3021-3028
Number of pages8
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume47
Issue number1
DOIs
Publication statusPublished - Dec 4 2019

Fingerprint

Cell Aging
Proteins
Phenotype
Phosphorylation
Sorting
Monolayers
Cells
Tissue
Nucleus Pulposus
Serial Passage
Glycogen Synthase Kinase 3
Catenins
Metalloproteases
Mitogen-Activated Protein Kinases
Matrix Metalloproteinases
Extracellular Matrix

Keywords

  • CD24
  • cell therapy
  • cellular senescence
  • Nucleus pulposus
  • phenotype

ASJC Scopus subject areas

  • Biotechnology
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

CD24 expression indicates healthier phenotype and less tendency of cellular senescence in human nucleus pulposus cells. / Yang, Shu Hua; Hu, Ming Hsiao; Wu, Chang Chin; Chen, Chih Wei; Sun, Yuan Hui; Yang, Kai Chiang.

In: Artificial Cells, Nanomedicine and Biotechnology, Vol. 47, No. 1, 04.12.2019, p. 3021-3028.

Research output: Contribution to journalArticle

Yang, Shu Hua ; Hu, Ming Hsiao ; Wu, Chang Chin ; Chen, Chih Wei ; Sun, Yuan Hui ; Yang, Kai Chiang. / CD24 expression indicates healthier phenotype and less tendency of cellular senescence in human nucleus pulposus cells. In: Artificial Cells, Nanomedicine and Biotechnology. 2019 ; Vol. 47, No. 1. pp. 3021-3028.
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