CD200 in growing rat lungs: developmental expression and control by dexamethasone

Ming Hsuan Tsai, Chin Chen Chu, Tsiu shan Wei, Mei Miao Chiu, Chiu-Yun Chang, I Hua Wei, Hsiung-Fei Chien, Hui Min Chen, Ching-Hsiang Wu, Jiang Shieh Ya Fen

Research output: Contribution to journalArticle

Abstract

CD200 belongs to cell adhesion molecules of the immunoglobulin superfamily. It lacks intracellular signaling motifs and exerts immunosuppressive effect in various tissues. We have reported previously that CD200 is predominantly associated with the capillary network in the alveolar septum of adult rats. The alveolar endothelial cells express CD200, which is confined to their luminal cell membrane facing the blood-air barrier. Our present results show that lung CD200 protein increases gradually with advancing age, being maximally expressed in the early postnatal (P) period. CD200 protein expression, however, declines at P5 but increases again after P7, reaching the adult level at P21. In developing lungs in fetal and neonatal stages, double-immunofluorescence staining has confirmed intense CD200 immunoreactivity delineating the vascular profiles in the double layers of the alveolar capillaries; this staining becomes diffuse and patchy with time. Unlike in adult lungs, immunoelectron microscopy has revealed that CD200 expression in fetal and early postnatal lungs is localized over the entire luminal cell membrane and in the cytoplasm of the endothelia. CD200 expression is progressively redistributed to a specific luminal domain of alveolar endothelia during pulmonary microvascular maturation. In neonatal rats treated with dexamethasone, the amount of lung CD200 significantly increases and is also elevated with time. Upregulation of endothelial CD200 has further been confirmed in isolated pulmonary microvascular endothelial cells treated with dexamethasone. Thus, lung CD200 is developmentally regulated, possibly under hormonal influence. © 2014, Springer-Verlag Berlin Heidelberg.
Original languageEnglish
Pages (from-to)729-742
Number of pages14
JournalCell and Tissue Research
Volume359
Issue number3
DOIs
Publication statusPublished - 2015

Cite this

Tsai, M. H., Chu, C. C., Wei, T. S., Chiu, M. M., Chang, C-Y., Wei, I. H., ... Ya Fen, J. S. (2015). CD200 in growing rat lungs: developmental expression and control by dexamethasone. Cell and Tissue Research, 359(3), 729-742. https://doi.org/10.1007/s00441-014-2065-8

CD200 in growing rat lungs: developmental expression and control by dexamethasone. / Tsai, Ming Hsuan; Chu, Chin Chen; Wei, Tsiu shan ; Chiu, Mei Miao; Chang, Chiu-Yun; Wei, I Hua; Chien, Hsiung-Fei; Chen, Hui Min; Wu, Ching-Hsiang; Ya Fen, Jiang Shieh.

In: Cell and Tissue Research, Vol. 359, No. 3, 2015, p. 729-742.

Research output: Contribution to journalArticle

Tsai, MH, Chu, CC, Wei, TS, Chiu, MM, Chang, C-Y, Wei, IH, Chien, H-F, Chen, HM, Wu, C-H & Ya Fen, JS 2015, 'CD200 in growing rat lungs: developmental expression and control by dexamethasone', Cell and Tissue Research, vol. 359, no. 3, pp. 729-742. https://doi.org/10.1007/s00441-014-2065-8
Tsai, Ming Hsuan ; Chu, Chin Chen ; Wei, Tsiu shan ; Chiu, Mei Miao ; Chang, Chiu-Yun ; Wei, I Hua ; Chien, Hsiung-Fei ; Chen, Hui Min ; Wu, Ching-Hsiang ; Ya Fen, Jiang Shieh. / CD200 in growing rat lungs: developmental expression and control by dexamethasone. In: Cell and Tissue Research. 2015 ; Vol. 359, No. 3. pp. 729-742.
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AU - Chu, Chin Chen

AU - Wei, Tsiu shan

AU - Chiu, Mei Miao

AU - Chang, Chiu-Yun

AU - Wei, I Hua

AU - Chien, Hsiung-Fei

AU - Chen, Hui Min

AU - Wu, Ching-Hsiang

AU - Ya Fen, Jiang Shieh

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AB - CD200 belongs to cell adhesion molecules of the immunoglobulin superfamily. It lacks intracellular signaling motifs and exerts immunosuppressive effect in various tissues. We have reported previously that CD200 is predominantly associated with the capillary network in the alveolar septum of adult rats. The alveolar endothelial cells express CD200, which is confined to their luminal cell membrane facing the blood-air barrier. Our present results show that lung CD200 protein increases gradually with advancing age, being maximally expressed in the early postnatal (P) period. CD200 protein expression, however, declines at P5 but increases again after P7, reaching the adult level at P21. In developing lungs in fetal and neonatal stages, double-immunofluorescence staining has confirmed intense CD200 immunoreactivity delineating the vascular profiles in the double layers of the alveolar capillaries; this staining becomes diffuse and patchy with time. Unlike in adult lungs, immunoelectron microscopy has revealed that CD200 expression in fetal and early postnatal lungs is localized over the entire luminal cell membrane and in the cytoplasm of the endothelia. CD200 expression is progressively redistributed to a specific luminal domain of alveolar endothelia during pulmonary microvascular maturation. In neonatal rats treated with dexamethasone, the amount of lung CD200 significantly increases and is also elevated with time. Upregulation of endothelial CD200 has further been confirmed in isolated pulmonary microvascular endothelial cells treated with dexamethasone. Thus, lung CD200 is developmentally regulated, possibly under hormonal influence. © 2014, Springer-Verlag Berlin Heidelberg.

KW - Alveolar endothelium

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KW - Development

KW - Dexamethasone

KW - Pulmonary

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