CD14 promoter polymorphism in Chinese alcoholic patients with cirrhosis of liver and acute pancreatitis

You-Chen Chao, Heng-Cheng Chu, Wei-Kuo Chang, Hsin-Hung Huang, Tsai-Yuan Hsieh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aim: To investigate the relationship between genetic polymorphism of the CD14 promoter and the occurrence of alcoholic cirrhosis and alcoholic pancreatitis, and to challenge the conclusion made earlier that the patients with acute alcoholic pancreatitis and patients with alcoholic cirrhosis of liver are two different subpopulations. Methods: Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, we determined the polymorphism of CD14 gene and aldehyde dehydrogenase gene 2 (ALDH 2) in 335 alcoholic patients with different organ complications i.e., cirrhosis of liver (n = 100), acute pancreatitis (n = 100), esophageal cancer (n = 82) and avascular necrosis of hip joint (AVN) (n = 53) and 194 non-alcoholic controls in a Chinese group. Results: The results showed that the carriage of T allele was not different among alcoholic patients with cirrhosis of liver, alcoholic patients with other complication and non-alcoholic controls. On the other hand, the carriage of the C allele was significantly more prevalent for alcoholic pancreatitis than for esophageal cancer (0.79 vs 0.60, P
Original languageEnglish
Pages (from-to)6043-6048
Number of pages6
JournalWorld Journal of Gastroenterology
Volume11
Issue number38
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Alcoholics
Liver Cirrhosis
Pancreatitis
Alcoholic Pancreatitis
Alcoholic Liver Cirrhosis
Esophageal Neoplasms
Alleles
Aldehyde Dehydrogenase
Hip Joint
Genetic Polymorphisms
Restriction Fragment Length Polymorphisms
Genes
Necrosis
Polymerase Chain Reaction

Keywords

  • Alcoholic patients
  • CD14 promoter
  • Polymorphism
  • aldehyde dehydrogenase isoenzyme 2
  • CD14 antigen
  • adult
  • alcohol liver cirrhosis
  • alcoholic pancreatitis
  • article
  • avascular necrosis
  • Chinese
  • controlled study
  • DNA polymorphism
  • esophagus cancer
  • female
  • gene frequency
  • genotype
  • heterozygote
  • human
  • major clinical study
  • male
  • pathogenesis
  • polymerase chain reaction
  • prevalence
  • promoter region
  • restriction fragment length polymorphism
  • statistical analysis
  • Adult
  • Aged
  • Antigens, CD14
  • Asian Continental Ancestry Group
  • Base Sequence
  • Case-Control Studies
  • DNA
  • Female
  • Humans
  • Liver Cirrhosis, Alcoholic
  • Male
  • Middle Aged
  • Pancreatitis, Alcoholic
  • Polymorphism, Genetic
  • Promoter Regions (Genetics)
  • Taiwan

Cite this

CD14 promoter polymorphism in Chinese alcoholic patients with cirrhosis of liver and acute pancreatitis. / Chao, You-Chen; Chu, Heng-Cheng; Chang, Wei-Kuo; Huang, Hsin-Hung; Hsieh, Tsai-Yuan.

In: World Journal of Gastroenterology, Vol. 11, No. 38, 2005, p. 6043-6048.

Research output: Contribution to journalArticle

Chao, You-Chen ; Chu, Heng-Cheng ; Chang, Wei-Kuo ; Huang, Hsin-Hung ; Hsieh, Tsai-Yuan. / CD14 promoter polymorphism in Chinese alcoholic patients with cirrhosis of liver and acute pancreatitis. In: World Journal of Gastroenterology. 2005 ; Vol. 11, No. 38. pp. 6043-6048.
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title = "CD14 promoter polymorphism in Chinese alcoholic patients with cirrhosis of liver and acute pancreatitis",
abstract = "Aim: To investigate the relationship between genetic polymorphism of the CD14 promoter and the occurrence of alcoholic cirrhosis and alcoholic pancreatitis, and to challenge the conclusion made earlier that the patients with acute alcoholic pancreatitis and patients with alcoholic cirrhosis of liver are two different subpopulations. Methods: Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, we determined the polymorphism of CD14 gene and aldehyde dehydrogenase gene 2 (ALDH 2) in 335 alcoholic patients with different organ complications i.e., cirrhosis of liver (n = 100), acute pancreatitis (n = 100), esophageal cancer (n = 82) and avascular necrosis of hip joint (AVN) (n = 53) and 194 non-alcoholic controls in a Chinese group. Results: The results showed that the carriage of T allele was not different among alcoholic patients with cirrhosis of liver, alcoholic patients with other complication and non-alcoholic controls. On the other hand, the carriage of the C allele was significantly more prevalent for alcoholic pancreatitis than for esophageal cancer (0.79 vs 0.60, P",
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author = "You-Chen Chao and Heng-Cheng Chu and Wei-Kuo Chang and Hsin-Hung Huang and Tsai-Yuan Hsieh",
note = "被引用次數:15 Export Date: 22 March 2016 CODEN: WJGAF 通訊地址: Chao, Y.-C.No. 325 Section 2, Cheng-Kung Road, Neihu 114 Taipei, Taiwan; 電子郵件: chaoycmd@ndmctsgh.edu.tw 化學物質/CAS: Antigens, CD14; DNA, 9007-49-2 參考文獻: Schuckit, M.A., Alcohol and alcoholism (1995) Harrison's Principles of Internal Medicine, pp. 2420-2425. , Braunwald E, Isselbacher KJ, Eds. 13th Ed. New York: McGraw-Hill; Hrubec, Z., Omenn, G.S., Evidence of genetic predisposition to alcoholic cirrhosis and psychosis: Twin concordances for alcoholism and its biological end points by zygosity among male veterans (1981) Alcohol Clin Exp Res, 5, pp. 207-215; Reed, T., Page, W.F., Viken, R.J., Christian, J.C., Genetic predisposition to organ-specific endpoints of alcoholism (1996) Alcohol Clin Exp Res, 20, pp. 1528-1533; Lieber, C.S., Alcohol and the liver: 1994 update (1994) Gastroenterology, 106, pp. 1085-1105; Bosron, W.F., Li, T.K., Genetic polymorphism of human liver alcohol and aldehyde dehydrogenase, and their relationship to alcohol metabolism and alcoholism (1986) Hepatology, 6, pp. 502-510; Bosron, W.F., Ehrig, T., Li, T.K., Genetic factors in alcohol metabolism and alcoholism (1993) Semin Liver Dis, 13, pp. 126-135; Goedde, H.W., Agarwal, D.P., Acetaldehyde metabolism: Genetic variation and physiological implications (1989) Alcoholism: Biomedical and Genetic Aspects, pp. 21-56. , Goedde HW, Agarwal DP, eds. Elmsford: Pergamon Press; Smith, M., Genetics of human alcohol and aldehyde dehydrogenases (1986) Adv Hum Genet, 15, pp. 249-290; Maezawa, Y., Yamauchi, M., Toda, G., Association between restriction fragment length polymorphism of the human cytochrome P450IIE1 gene and susceptibility to alcoholic liver cirrhosis (1994) Am J Gastroenterol, 89, pp. 561-565; Tsutsumi, M., Takada, A., Wang, J.S., Genetic polymorphisms of cytochrome P4502E1 related to the development of alcoholic liver disease (1994) Gastroenterology, 107, pp. 1430-1435; Day, C.P., James, O.F., Bassendine, M.F., Crabb, D.W., Li, T.K., Alcohol dehydrogenase polymorphisms and predisposition to alcoholic cirrhosis (1993) Hepatology, 18, pp. 230-232; Crabb, D.W., Ethanol oxidizing enzymes: Roles in alcohol metabolism and alcoholic liver disease (1995) Prog Liver Dis, 13, pp. 151-172; Enomoto, N., Takase, S., Takada, N., Alcoholic liver disease in heterozygotes of mutant and normal aldehyde dehydrogenase-2 genes (1991) Hepatology, 13, pp. 1071-1075; Chao, Y.C., Liou, S.R., Chung, Y.Y., Tang, H.S., Hsu, C.T., Li, T.K., Yin, S.J., Polymorphism of alcohol and aldehyde dehydrogenase genes and alcoholic cirrhosis in Chinese patients (1994) Hepatology, 19, pp. 360-366; Chao, Y.C., Young, T.H., Chang, W.K., Tang, H.S., Hsu, C.T., An investigation of whether polymorphisms of cytochrome P4502E1 are genetic markers of susceptibility to alcoholic end-stage organ damage in a Chinese population (1995) Hepatology, 22, pp. 1409-1414; Yamauchi, M., Maezawa, Y., Mizuhara, Y., Ohata, M., Hirakawa, J., Nakajima, H., Toda, G., Polymorphisms in alcohol metabolizing enzyme genes and alcoholic cirrhosis in Japanese patients: A multivariate analysis (1995) Hepatology, 22, pp. 1136-1142; Bode, C., Kugler, V., Bode, J.C., Endotoxemia in patients with alcoholic and non-alcoholic cirrhosis and in subjects with no evidence of chronic liver disease following acute alcohol excess (1987) J Hepatol, 4, pp. 8-14; Nanji, A.A., Khettry, U., Sadrzadeh, S.M., Yamanaka, T., Severity of liver injury in experimental alcoholic liver disease. Correlation with plasma endotoxin, prostaglandin E2, leukotriene B4, and thromboxane B2 (1993) Am J Pathol, 142, pp. 367-373; Schenker, S., Bay, M.K., Alcohol and endotoxin: Another path to alcoholic liver injury? (1995) Alcohol Clin Exp Res, 19, pp. 1364-1366; Matsuura, K., Ishida, T., Setoguchi, M., Higuchi, Y., Akizuki, S., Yamamoto, S., Upregulation of mouth CD14 expression in Kupffer cells by lipopolysaccharide (1994) J Exp Med, 179, pp. 1671-1676; Kielian, T.L., Blecha, F., CD 14 and other recognition molecules for lipopolysaccharide: A review (1995) Immunopharmacology, 29, pp. 187-205; Baldini, M., Lohman, I.C., Halonen, M., Erickson, R.P., Holt, P.G., Martinez, F.D., Polymorphism in the 5′ flanking region of the CD 14 gene is associated with circulating soluble CD14 levels and with total serum immunoglobulin E (1999) Am J Respir Cell Mol Biol, 20, pp. 976-983; Hubacek, J.A., Rothe, G., Pit'ha, J., Skodova, Z., Stanek, V., Poledne, R., Schmitz, G., C(-260)T polymorphism in the promoter of the CD14 monocyte receptor gene as a risk factor for myocardial infarction (1999) Circulation, 99, pp. 3218-3220; Unkelbach, K., Gardemann, A., Kostrzewa, M., Philipp, M., Tillmanns, H., Haberbosch, W., A new promoter polymorphism in the gene of lipopolysaccharide receptor CD14 is associated with expired myocardial infarction in patients with low atherosclerotic risk profile (1999) Arterioscler Thromb Vasc Biol, 19, pp. 932-938; Jarvelainen, H.A., Orpana, A., Perola, M., Savolainen, V.T., Karhunen, P.J., Lindros, K.O., Promoter polymorphism of the CD14 endotoxin receptor gene as a risk factor for alcoholic liver disease (2001) Hepatology, 33, pp. 1148-1153; Koppelman, G.H., Reijmerink, N.E., Colin Stine, O., Howard, T.D., Whittaker, P.A., Meyers, D.A., Postma, D.S., Bleecker, E.R., Association of a promoter polymorphism of the CD14 gene and atopy (2001) Am J Respir Crit Care Med, 163, pp. 965-969; Chao, Y.C., Young, T.H., Tang, H.S., Hsu, C.T., Alcoholism and alcoholic organ damage and genetic polymorphisms of alcohol metabolizing enzymes in Chinese patients (1997) Hepatology, 25, pp. 112-117; Ito, D., Murata, M., Tanahashi, N., Sato, H., Sonoda, A., Saito, I., Watanabe, K., Fukuuchi, Y., Polymorphism in the promoter of lipopolysaccharide receptor CD14 and ischemic cerebrovascular disease (2000) Stroke, 31, pp. 2661-2664; Risley, P., Jerrard-Dunne, P., Sitzer, M., Buehler, A., von Kegler, S., Markus, H.S., Carotid Atherosclerosis Progression Study. Promoter polymorphism in the endotoxin receptor (CD14) is associated with increased carotid atherosclerosis only in smokers. The carotid atherosclerosis progression study (CAPS) (2003) Stroke, 34, pp. 604-606; Fernandez-Real, J.M., Broch, M., Richart, C., Vendrell, J., Lopez-Bermejo, A., Ricart, W., CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity (2003) J Clin Endocrinol Metab, 88, pp. 1780-1784; Koch, W., Kastrati, A., Mehilli, J., von Beckerath, N., Schomig, A., CD14 gene -159 C/T polymorphism is not associated with coronary artery disease and myocardial infarction (2002) Am Heart J, 143, pp. 971-976; Schlegel, R.A., Krahling, S., Callahan, M.K., Williamson, P., CD14 is a component of multiple recognition systems used by macrophages to phagocytose apoptotic lymphocytes (1999) Cell Death Differ, 6, pp. 583-592; Gibot, S., Cariou, A., Drouet, L., Rossignol, M., Ripoll, L., Association between a genomic polymorphism within the CD14 locus and septic shock susceptibility and mortality rate (2002) Crit Care Med, 30, pp. 969-973; Obana, N., Takahashi, S., Kinouchi, Y., Negoro, K., Takagi, S., Hiwatashi, N., Shimosegawa, T., Ulcerative colitis is associated with a promoter polymorphism of lipopolysaccharide receptor gene, CD14 (2002) Scand J Gastroenterol, 37, pp. 699-704; Klein, W., Tromm, A., Griga, T., Fricke, H., Folwaczny, C., Hocke, M., Eitner, K., Epplen, J.T., A polymorphism in the CD14 gene is associated with Crohn disease (2002) Scand J Gastroenterol, 37, pp. 189-191",
year = "2005",
language = "English",
volume = "11",
pages = "6043--6048",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "38",

}

TY - JOUR

T1 - CD14 promoter polymorphism in Chinese alcoholic patients with cirrhosis of liver and acute pancreatitis

AU - Chao, You-Chen

AU - Chu, Heng-Cheng

AU - Chang, Wei-Kuo

AU - Huang, Hsin-Hung

AU - Hsieh, Tsai-Yuan

N1 - 被引用次數:15 Export Date: 22 March 2016 CODEN: WJGAF 通訊地址: Chao, Y.-C.No. 325 Section 2, Cheng-Kung Road, Neihu 114 Taipei, Taiwan; 電子郵件: chaoycmd@ndmctsgh.edu.tw 化學物質/CAS: Antigens, CD14; DNA, 9007-49-2 參考文獻: Schuckit, M.A., Alcohol and alcoholism (1995) Harrison's Principles of Internal Medicine, pp. 2420-2425. , Braunwald E, Isselbacher KJ, Eds. 13th Ed. New York: McGraw-Hill; Hrubec, Z., Omenn, G.S., Evidence of genetic predisposition to alcoholic cirrhosis and psychosis: Twin concordances for alcoholism and its biological end points by zygosity among male veterans (1981) Alcohol Clin Exp Res, 5, pp. 207-215; Reed, T., Page, W.F., Viken, R.J., Christian, J.C., Genetic predisposition to organ-specific endpoints of alcoholism (1996) Alcohol Clin Exp Res, 20, pp. 1528-1533; Lieber, C.S., Alcohol and the liver: 1994 update (1994) Gastroenterology, 106, pp. 1085-1105; Bosron, W.F., Li, T.K., Genetic polymorphism of human liver alcohol and aldehyde dehydrogenase, and their relationship to alcohol metabolism and alcoholism (1986) Hepatology, 6, pp. 502-510; Bosron, W.F., Ehrig, T., Li, T.K., Genetic factors in alcohol metabolism and alcoholism (1993) Semin Liver Dis, 13, pp. 126-135; Goedde, H.W., Agarwal, D.P., Acetaldehyde metabolism: Genetic variation and physiological implications (1989) Alcoholism: Biomedical and Genetic Aspects, pp. 21-56. , Goedde HW, Agarwal DP, eds. Elmsford: Pergamon Press; Smith, M., Genetics of human alcohol and aldehyde dehydrogenases (1986) Adv Hum Genet, 15, pp. 249-290; Maezawa, Y., Yamauchi, M., Toda, G., Association between restriction fragment length polymorphism of the human cytochrome P450IIE1 gene and susceptibility to alcoholic liver cirrhosis (1994) Am J Gastroenterol, 89, pp. 561-565; Tsutsumi, M., Takada, A., Wang, J.S., Genetic polymorphisms of cytochrome P4502E1 related to the development of alcoholic liver disease (1994) Gastroenterology, 107, pp. 1430-1435; Day, C.P., James, O.F., Bassendine, M.F., Crabb, D.W., Li, T.K., Alcohol dehydrogenase polymorphisms and predisposition to alcoholic cirrhosis (1993) Hepatology, 18, pp. 230-232; Crabb, D.W., Ethanol oxidizing enzymes: Roles in alcohol metabolism and alcoholic liver disease (1995) Prog Liver Dis, 13, pp. 151-172; Enomoto, N., Takase, S., Takada, N., Alcoholic liver disease in heterozygotes of mutant and normal aldehyde dehydrogenase-2 genes (1991) Hepatology, 13, pp. 1071-1075; Chao, Y.C., Liou, S.R., Chung, Y.Y., Tang, H.S., Hsu, C.T., Li, T.K., Yin, S.J., Polymorphism of alcohol and aldehyde dehydrogenase genes and alcoholic cirrhosis in Chinese patients (1994) Hepatology, 19, pp. 360-366; Chao, Y.C., Young, T.H., Chang, W.K., Tang, H.S., Hsu, C.T., An investigation of whether polymorphisms of cytochrome P4502E1 are genetic markers of susceptibility to alcoholic end-stage organ damage in a Chinese population (1995) Hepatology, 22, pp. 1409-1414; Yamauchi, M., Maezawa, Y., Mizuhara, Y., Ohata, M., Hirakawa, J., Nakajima, H., Toda, G., Polymorphisms in alcohol metabolizing enzyme genes and alcoholic cirrhosis in Japanese patients: A multivariate analysis (1995) Hepatology, 22, pp. 1136-1142; Bode, C., Kugler, V., Bode, J.C., Endotoxemia in patients with alcoholic and non-alcoholic cirrhosis and in subjects with no evidence of chronic liver disease following acute alcohol excess (1987) J Hepatol, 4, pp. 8-14; Nanji, A.A., Khettry, U., Sadrzadeh, S.M., Yamanaka, T., Severity of liver injury in experimental alcoholic liver disease. Correlation with plasma endotoxin, prostaglandin E2, leukotriene B4, and thromboxane B2 (1993) Am J Pathol, 142, pp. 367-373; Schenker, S., Bay, M.K., Alcohol and endotoxin: Another path to alcoholic liver injury? (1995) Alcohol Clin Exp Res, 19, pp. 1364-1366; Matsuura, K., Ishida, T., Setoguchi, M., Higuchi, Y., Akizuki, S., Yamamoto, S., Upregulation of mouth CD14 expression in Kupffer cells by lipopolysaccharide (1994) J Exp Med, 179, pp. 1671-1676; Kielian, T.L., Blecha, F., CD 14 and other recognition molecules for lipopolysaccharide: A review (1995) Immunopharmacology, 29, pp. 187-205; Baldini, M., Lohman, I.C., Halonen, M., Erickson, R.P., Holt, P.G., Martinez, F.D., Polymorphism in the 5′ flanking region of the CD 14 gene is associated with circulating soluble CD14 levels and with total serum immunoglobulin E (1999) Am J Respir Cell Mol Biol, 20, pp. 976-983; Hubacek, J.A., Rothe, G., Pit'ha, J., Skodova, Z., Stanek, V., Poledne, R., Schmitz, G., C(-260)T polymorphism in the promoter of the CD14 monocyte receptor gene as a risk factor for myocardial infarction (1999) Circulation, 99, pp. 3218-3220; Unkelbach, K., Gardemann, A., Kostrzewa, M., Philipp, M., Tillmanns, H., Haberbosch, W., A new promoter polymorphism in the gene of lipopolysaccharide receptor CD14 is associated with expired myocardial infarction in patients with low atherosclerotic risk profile (1999) Arterioscler Thromb Vasc Biol, 19, pp. 932-938; Jarvelainen, H.A., Orpana, A., Perola, M., Savolainen, V.T., Karhunen, P.J., Lindros, K.O., Promoter polymorphism of the CD14 endotoxin receptor gene as a risk factor for alcoholic liver disease (2001) Hepatology, 33, pp. 1148-1153; Koppelman, G.H., Reijmerink, N.E., Colin Stine, O., Howard, T.D., Whittaker, P.A., Meyers, D.A., Postma, D.S., Bleecker, E.R., Association of a promoter polymorphism of the CD14 gene and atopy (2001) Am J Respir Crit Care Med, 163, pp. 965-969; Chao, Y.C., Young, T.H., Tang, H.S., Hsu, C.T., Alcoholism and alcoholic organ damage and genetic polymorphisms of alcohol metabolizing enzymes in Chinese patients (1997) Hepatology, 25, pp. 112-117; Ito, D., Murata, M., Tanahashi, N., Sato, H., Sonoda, A., Saito, I., Watanabe, K., Fukuuchi, Y., Polymorphism in the promoter of lipopolysaccharide receptor CD14 and ischemic cerebrovascular disease (2000) Stroke, 31, pp. 2661-2664; Risley, P., Jerrard-Dunne, P., Sitzer, M., Buehler, A., von Kegler, S., Markus, H.S., Carotid Atherosclerosis Progression Study. Promoter polymorphism in the endotoxin receptor (CD14) is associated with increased carotid atherosclerosis only in smokers. The carotid atherosclerosis progression study (CAPS) (2003) Stroke, 34, pp. 604-606; Fernandez-Real, J.M., Broch, M., Richart, C., Vendrell, J., Lopez-Bermejo, A., Ricart, W., CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity (2003) J Clin Endocrinol Metab, 88, pp. 1780-1784; Koch, W., Kastrati, A., Mehilli, J., von Beckerath, N., Schomig, A., CD14 gene -159 C/T polymorphism is not associated with coronary artery disease and myocardial infarction (2002) Am Heart J, 143, pp. 971-976; Schlegel, R.A., Krahling, S., Callahan, M.K., Williamson, P., CD14 is a component of multiple recognition systems used by macrophages to phagocytose apoptotic lymphocytes (1999) Cell Death Differ, 6, pp. 583-592; Gibot, S., Cariou, A., Drouet, L., Rossignol, M., Ripoll, L., Association between a genomic polymorphism within the CD14 locus and septic shock susceptibility and mortality rate (2002) Crit Care Med, 30, pp. 969-973; Obana, N., Takahashi, S., Kinouchi, Y., Negoro, K., Takagi, S., Hiwatashi, N., Shimosegawa, T., Ulcerative colitis is associated with a promoter polymorphism of lipopolysaccharide receptor gene, CD14 (2002) Scand J Gastroenterol, 37, pp. 699-704; Klein, W., Tromm, A., Griga, T., Fricke, H., Folwaczny, C., Hocke, M., Eitner, K., Epplen, J.T., A polymorphism in the CD14 gene is associated with Crohn disease (2002) Scand J Gastroenterol, 37, pp. 189-191

PY - 2005

Y1 - 2005

N2 - Aim: To investigate the relationship between genetic polymorphism of the CD14 promoter and the occurrence of alcoholic cirrhosis and alcoholic pancreatitis, and to challenge the conclusion made earlier that the patients with acute alcoholic pancreatitis and patients with alcoholic cirrhosis of liver are two different subpopulations. Methods: Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, we determined the polymorphism of CD14 gene and aldehyde dehydrogenase gene 2 (ALDH 2) in 335 alcoholic patients with different organ complications i.e., cirrhosis of liver (n = 100), acute pancreatitis (n = 100), esophageal cancer (n = 82) and avascular necrosis of hip joint (AVN) (n = 53) and 194 non-alcoholic controls in a Chinese group. Results: The results showed that the carriage of T allele was not different among alcoholic patients with cirrhosis of liver, alcoholic patients with other complication and non-alcoholic controls. On the other hand, the carriage of the C allele was significantly more prevalent for alcoholic pancreatitis than for esophageal cancer (0.79 vs 0.60, P

AB - Aim: To investigate the relationship between genetic polymorphism of the CD14 promoter and the occurrence of alcoholic cirrhosis and alcoholic pancreatitis, and to challenge the conclusion made earlier that the patients with acute alcoholic pancreatitis and patients with alcoholic cirrhosis of liver are two different subpopulations. Methods: Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, we determined the polymorphism of CD14 gene and aldehyde dehydrogenase gene 2 (ALDH 2) in 335 alcoholic patients with different organ complications i.e., cirrhosis of liver (n = 100), acute pancreatitis (n = 100), esophageal cancer (n = 82) and avascular necrosis of hip joint (AVN) (n = 53) and 194 non-alcoholic controls in a Chinese group. Results: The results showed that the carriage of T allele was not different among alcoholic patients with cirrhosis of liver, alcoholic patients with other complication and non-alcoholic controls. On the other hand, the carriage of the C allele was significantly more prevalent for alcoholic pancreatitis than for esophageal cancer (0.79 vs 0.60, P

KW - Alcoholic patients

KW - CD14 promoter

KW - Polymorphism

KW - aldehyde dehydrogenase isoenzyme 2

KW - CD14 antigen

KW - adult

KW - alcohol liver cirrhosis

KW - alcoholic pancreatitis

KW - article

KW - avascular necrosis

KW - Chinese

KW - controlled study

KW - DNA polymorphism

KW - esophagus cancer

KW - female

KW - gene frequency

KW - genotype

KW - heterozygote

KW - human

KW - major clinical study

KW - male

KW - pathogenesis

KW - polymerase chain reaction

KW - prevalence

KW - promoter region

KW - restriction fragment length polymorphism

KW - statistical analysis

KW - Adult

KW - Aged

KW - Antigens, CD14

KW - Asian Continental Ancestry Group

KW - Base Sequence

KW - Case-Control Studies

KW - DNA

KW - Female

KW - Humans

KW - Liver Cirrhosis, Alcoholic

KW - Male

KW - Middle Aged

KW - Pancreatitis, Alcoholic

KW - Polymorphism, Genetic

KW - Promoter Regions (Genetics)

KW - Taiwan

M3 - Article

VL - 11

SP - 6043

EP - 6048

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 38

ER -