Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold

Chen Chie Wang, Kai Chiang Yang, Keng Hui Lin, Yen Liang Liu, Hwa Chang Liu, Feng Huei Lin

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Tissue engineering for cartilage regeneration provides an alternative to surgery for degenerative osteoarthritis. Recently, a highly organized three-dimensional (3D) alginate scaffold was prepared using a microfluidic device; this scaffold is effective for chondrocyte culture in vitro. The performance of this scaffold was further demonstrated; an alginate scaffold seeded with porcine chondrocytes was implanted in the dorsal subcutaneous site of SCID mice. The recipients were sacrificed at 2, 4, and 6 weeks after transplantation. The grafted implants retrieved from the subcutaneous site were analyzed with histologic examinations. Real-time PCR was used to identify the gene expression patterns of the chondrocytes. The hematoxylin and eosin staining showed that the chondrocytes survived normally in SCID mice; cartilage-like structures were formed after 4 weeks implantation. Immunohistochemical staining revealed cells secreted type II collagen, produced glycosaminoglycans (proved by alcian blue stain), and maintained the expression of S-100. On the other hand, the cells were negative for type I and type X collagen staining. PCR showed that the mRNA expressions of aggrecan and type II collagen were up-regulated at weeks two and four, while type I and type X collagen were down-regulated during the study period. In summary, this highly organized 3D alginate scaffold provided a suitable environment and maintained functional phenotypes for chondrocytes in this animal study.

Original languageEnglish
Pages (from-to)120-127
Number of pages8
JournalBiomaterials
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

SCID Mice
Alginate
Cartilage
Scaffolds (biology)
Chondrocytes
Scaffolds
Regeneration
Collagen
Collagen Type X
Collagen Type II
Lab-On-A-Chip Devices
Staining and Labeling
Collagen Type I
Aggrecans
Alcian Blue
Hematoxylin
Tissue Engineering
Eosine Yellowish-(YS)
Glycosaminoglycans
Tissue engineering

Keywords

  • Alginate
  • Cartilage tissue engineering
  • Chondrocyte
  • Scaffold
  • SCID mouse

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold. / Wang, Chen Chie; Yang, Kai Chiang; Lin, Keng Hui; Liu, Yen Liang; Liu, Hwa Chang; Lin, Feng Huei.

In: Biomaterials, Vol. 33, No. 1, 01.2012, p. 120-127.

Research output: Contribution to journalArticle

Wang, Chen Chie ; Yang, Kai Chiang ; Lin, Keng Hui ; Liu, Yen Liang ; Liu, Hwa Chang ; Lin, Feng Huei. / Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold. In: Biomaterials. 2012 ; Vol. 33, No. 1. pp. 120-127.
@article{cdf2594749cd47a8accbe3968e15adc2,
title = "Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold",
abstract = "Tissue engineering for cartilage regeneration provides an alternative to surgery for degenerative osteoarthritis. Recently, a highly organized three-dimensional (3D) alginate scaffold was prepared using a microfluidic device; this scaffold is effective for chondrocyte culture in vitro. The performance of this scaffold was further demonstrated; an alginate scaffold seeded with porcine chondrocytes was implanted in the dorsal subcutaneous site of SCID mice. The recipients were sacrificed at 2, 4, and 6 weeks after transplantation. The grafted implants retrieved from the subcutaneous site were analyzed with histologic examinations. Real-time PCR was used to identify the gene expression patterns of the chondrocytes. The hematoxylin and eosin staining showed that the chondrocytes survived normally in SCID mice; cartilage-like structures were formed after 4 weeks implantation. Immunohistochemical staining revealed cells secreted type II collagen, produced glycosaminoglycans (proved by alcian blue stain), and maintained the expression of S-100. On the other hand, the cells were negative for type I and type X collagen staining. PCR showed that the mRNA expressions of aggrecan and type II collagen were up-regulated at weeks two and four, while type I and type X collagen were down-regulated during the study period. In summary, this highly organized 3D alginate scaffold provided a suitable environment and maintained functional phenotypes for chondrocytes in this animal study.",
keywords = "Alginate, Cartilage tissue engineering, Chondrocyte, Scaffold, SCID mouse",
author = "Wang, {Chen Chie} and Yang, {Kai Chiang} and Lin, {Keng Hui} and Liu, {Yen Liang} and Liu, {Hwa Chang} and Lin, {Feng Huei}",
year = "2012",
month = "1",
doi = "10.1016/j.biomaterials.2011.09.042",
language = "English",
volume = "33",
pages = "120--127",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Science Ltd",
number = "1",

}

TY - JOUR

T1 - Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold

AU - Wang, Chen Chie

AU - Yang, Kai Chiang

AU - Lin, Keng Hui

AU - Liu, Yen Liang

AU - Liu, Hwa Chang

AU - Lin, Feng Huei

PY - 2012/1

Y1 - 2012/1

N2 - Tissue engineering for cartilage regeneration provides an alternative to surgery for degenerative osteoarthritis. Recently, a highly organized three-dimensional (3D) alginate scaffold was prepared using a microfluidic device; this scaffold is effective for chondrocyte culture in vitro. The performance of this scaffold was further demonstrated; an alginate scaffold seeded with porcine chondrocytes was implanted in the dorsal subcutaneous site of SCID mice. The recipients were sacrificed at 2, 4, and 6 weeks after transplantation. The grafted implants retrieved from the subcutaneous site were analyzed with histologic examinations. Real-time PCR was used to identify the gene expression patterns of the chondrocytes. The hematoxylin and eosin staining showed that the chondrocytes survived normally in SCID mice; cartilage-like structures were formed after 4 weeks implantation. Immunohistochemical staining revealed cells secreted type II collagen, produced glycosaminoglycans (proved by alcian blue stain), and maintained the expression of S-100. On the other hand, the cells were negative for type I and type X collagen staining. PCR showed that the mRNA expressions of aggrecan and type II collagen were up-regulated at weeks two and four, while type I and type X collagen were down-regulated during the study period. In summary, this highly organized 3D alginate scaffold provided a suitable environment and maintained functional phenotypes for chondrocytes in this animal study.

AB - Tissue engineering for cartilage regeneration provides an alternative to surgery for degenerative osteoarthritis. Recently, a highly organized three-dimensional (3D) alginate scaffold was prepared using a microfluidic device; this scaffold is effective for chondrocyte culture in vitro. The performance of this scaffold was further demonstrated; an alginate scaffold seeded with porcine chondrocytes was implanted in the dorsal subcutaneous site of SCID mice. The recipients were sacrificed at 2, 4, and 6 weeks after transplantation. The grafted implants retrieved from the subcutaneous site were analyzed with histologic examinations. Real-time PCR was used to identify the gene expression patterns of the chondrocytes. The hematoxylin and eosin staining showed that the chondrocytes survived normally in SCID mice; cartilage-like structures were formed after 4 weeks implantation. Immunohistochemical staining revealed cells secreted type II collagen, produced glycosaminoglycans (proved by alcian blue stain), and maintained the expression of S-100. On the other hand, the cells were negative for type I and type X collagen staining. PCR showed that the mRNA expressions of aggrecan and type II collagen were up-regulated at weeks two and four, while type I and type X collagen were down-regulated during the study period. In summary, this highly organized 3D alginate scaffold provided a suitable environment and maintained functional phenotypes for chondrocytes in this animal study.

KW - Alginate

KW - Cartilage tissue engineering

KW - Chondrocyte

KW - Scaffold

KW - SCID mouse

UR - http://www.scopus.com/inward/record.url?scp=82855172103&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82855172103&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2011.09.042

DO - 10.1016/j.biomaterials.2011.09.042

M3 - Article

VL - 33

SP - 120

EP - 127

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 1

ER -