Cartilage fragments from osteoarthritic knee promote chondrogenesis of mesenchymal stem cells without exogenous growth factor induction.

Chia Chun Chen, Cheng Hao Liao, Yao Horng Wang, Yuan Ming Hsu, Shih Horng Huang, Chih Hung Chang, Hsu Wei Fang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Extracellular matrix (ECM) is thought to participate significantly in guiding the differentiation process of mesenchymal stem cells (MSCs). In this study, we hypothesized that cartilage fragments from osteoarthritic knee could promote chondrogenesis of MSCs. Nonworn parts of cartilage tissues were obtained during total knee arthroplasty (TKA) surgery. Cartilage fragments and MSCs were wrapped into fibrin glue; and the constructs were implanted subcutaneously into nude mice. Histological analysis showed neocartilage-like structure with positive Alcian blue staining in the cartilage fragment-fibrin-MSC constructs. However, constructs with only MSCs in fibrin showed condensed appearance like MSCs in the pellet culture. Gene expression of type II collagen in the constructs with 60 mg cartilage fragments were significantly elevated after 4 weeks of implantation. Conversely, the constructs without cartilage fragments failed to express type II collagen, which indicated MSCs did not differentiate into a chondrogenic lineage. In conclusion, we demonstrated the effect of cartilage fragments from osteoarthritic knee in promoting chondrogenic differentiation of MSCs. This may be a favorable strategy for MSC chondrogenesis without exogenous growth factor induction.

Original languageEnglish
Pages (from-to)393-400
Number of pages8
JournalJournal of orthopaedic research : official publication of the Orthopaedic Research Society
Volume30
Issue number3
DOIs
Publication statusPublished - Jan 1 2012
Externally publishedYes

Fingerprint

Chondrogenesis
Mesenchymal Stromal Cells
Cartilage
Knee
Intercellular Signaling Peptides and Proteins
Collagen Type II
Fibrin
Alcian Blue
Knee Replacement Arthroplasties
Fibrin Tissue Adhesive
Nude Mice
Extracellular Matrix
Staining and Labeling
Gene Expression

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Cartilage fragments from osteoarthritic knee promote chondrogenesis of mesenchymal stem cells without exogenous growth factor induction. / Chen, Chia Chun; Liao, Cheng Hao; Wang, Yao Horng; Hsu, Yuan Ming; Huang, Shih Horng; Chang, Chih Hung; Fang, Hsu Wei.

In: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 30, No. 3, 01.01.2012, p. 393-400.

Research output: Contribution to journalArticle

@article{59151a1ce45b4aa8a816e9db2fd31b2a,
title = "Cartilage fragments from osteoarthritic knee promote chondrogenesis of mesenchymal stem cells without exogenous growth factor induction.",
abstract = "Extracellular matrix (ECM) is thought to participate significantly in guiding the differentiation process of mesenchymal stem cells (MSCs). In this study, we hypothesized that cartilage fragments from osteoarthritic knee could promote chondrogenesis of MSCs. Nonworn parts of cartilage tissues were obtained during total knee arthroplasty (TKA) surgery. Cartilage fragments and MSCs were wrapped into fibrin glue; and the constructs were implanted subcutaneously into nude mice. Histological analysis showed neocartilage-like structure with positive Alcian blue staining in the cartilage fragment-fibrin-MSC constructs. However, constructs with only MSCs in fibrin showed condensed appearance like MSCs in the pellet culture. Gene expression of type II collagen in the constructs with 60 mg cartilage fragments were significantly elevated after 4 weeks of implantation. Conversely, the constructs without cartilage fragments failed to express type II collagen, which indicated MSCs did not differentiate into a chondrogenic lineage. In conclusion, we demonstrated the effect of cartilage fragments from osteoarthritic knee in promoting chondrogenic differentiation of MSCs. This may be a favorable strategy for MSC chondrogenesis without exogenous growth factor induction.",
author = "Chen, {Chia Chun} and Liao, {Cheng Hao} and Wang, {Yao Horng} and Hsu, {Yuan Ming} and Huang, {Shih Horng} and Chang, {Chih Hung} and Fang, {Hsu Wei}",
year = "2012",
month = "1",
day = "1",
doi = "10.1002/jor.21541",
language = "English",
volume = "30",
pages = "393--400",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Cartilage fragments from osteoarthritic knee promote chondrogenesis of mesenchymal stem cells without exogenous growth factor induction.

AU - Chen, Chia Chun

AU - Liao, Cheng Hao

AU - Wang, Yao Horng

AU - Hsu, Yuan Ming

AU - Huang, Shih Horng

AU - Chang, Chih Hung

AU - Fang, Hsu Wei

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Extracellular matrix (ECM) is thought to participate significantly in guiding the differentiation process of mesenchymal stem cells (MSCs). In this study, we hypothesized that cartilage fragments from osteoarthritic knee could promote chondrogenesis of MSCs. Nonworn parts of cartilage tissues were obtained during total knee arthroplasty (TKA) surgery. Cartilage fragments and MSCs were wrapped into fibrin glue; and the constructs were implanted subcutaneously into nude mice. Histological analysis showed neocartilage-like structure with positive Alcian blue staining in the cartilage fragment-fibrin-MSC constructs. However, constructs with only MSCs in fibrin showed condensed appearance like MSCs in the pellet culture. Gene expression of type II collagen in the constructs with 60 mg cartilage fragments were significantly elevated after 4 weeks of implantation. Conversely, the constructs without cartilage fragments failed to express type II collagen, which indicated MSCs did not differentiate into a chondrogenic lineage. In conclusion, we demonstrated the effect of cartilage fragments from osteoarthritic knee in promoting chondrogenic differentiation of MSCs. This may be a favorable strategy for MSC chondrogenesis without exogenous growth factor induction.

AB - Extracellular matrix (ECM) is thought to participate significantly in guiding the differentiation process of mesenchymal stem cells (MSCs). In this study, we hypothesized that cartilage fragments from osteoarthritic knee could promote chondrogenesis of MSCs. Nonworn parts of cartilage tissues were obtained during total knee arthroplasty (TKA) surgery. Cartilage fragments and MSCs were wrapped into fibrin glue; and the constructs were implanted subcutaneously into nude mice. Histological analysis showed neocartilage-like structure with positive Alcian blue staining in the cartilage fragment-fibrin-MSC constructs. However, constructs with only MSCs in fibrin showed condensed appearance like MSCs in the pellet culture. Gene expression of type II collagen in the constructs with 60 mg cartilage fragments were significantly elevated after 4 weeks of implantation. Conversely, the constructs without cartilage fragments failed to express type II collagen, which indicated MSCs did not differentiate into a chondrogenic lineage. In conclusion, we demonstrated the effect of cartilage fragments from osteoarthritic knee in promoting chondrogenic differentiation of MSCs. This may be a favorable strategy for MSC chondrogenesis without exogenous growth factor induction.

UR - http://www.scopus.com/inward/record.url?scp=80052112420&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052112420&partnerID=8YFLogxK

U2 - 10.1002/jor.21541

DO - 10.1002/jor.21541

M3 - Article

VL - 30

SP - 393

EP - 400

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

IS - 3

ER -