Carriage rates of methicillin-resistant Staphylococcus aureus (MRSA) depend on anatomic location, the number of sites cultured, culture methods, and the distribution of clonotypes

T. L Y Lauderdale, J. T. Wang, W. S. Lee, J. H. Huang, L. C. McDonald, I. W. Huang, S. C. Chang

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Abstract

The present study was carried out to determine how active surveillance for methicillin-resistant Staphylococcus aureus (MRSA) could be improved by the use of enrichment broth and the inclusion of extra-nasal sites with nares cultures. Molecular typing was also performed to identify colonization by single or multiple strains. Surveillance cultures for MRSA were obtained from 650 patients on admission to a medical and surgical intensive care unit (ICU) in Taiwan. MRSA was detected on directly plated vs. broth-enrichment cultures in any site at 10.0% vs. 24.2%, nares 8.2% vs. 17.5%, throat 4.8% vs. 13.4%, axilla 1.2% vs. 9.1%, and perineum 1.8% vs. 9.5%, respectively. Nares cultures alone detected only 81.5% and 72.5% of all colonized patients by direct and broth-enriched cultures, respectively. The molecular typing of 68 isolates from 17 patients revealed that multisite isolates were largely indistinguishable within each patient, but four patients had multiple subtypes and another three patients had different clonotypes. The detection of MRSA carriers was considerably enhanced by broth-enrichment cultures at multiple anatomic sites and simultaneous colonization by multiple strains at different sites can occur. Epidemiological studies are needed to determine the likelihood of subsequent nosocomial infection among colonized patients detected via direct nasal versus broth-enriched cultures from multiple sites.

Original languageEnglish
Pages (from-to)1553-1559
Number of pages7
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume29
Issue number12
DOIs
Publication statusPublished - Dec 2010

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Methicillin-Resistant Staphylococcus aureus
Molecular Typing
Nose
Perineum
Axilla
Patient Admission
Critical Care
Cross Infection
Pharynx
Taiwan
Intensive Care Units
Epidemiologic Studies

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Carriage rates of methicillin-resistant Staphylococcus aureus (MRSA) depend on anatomic location, the number of sites cultured, culture methods, and the distribution of clonotypes. / Lauderdale, T. L Y; Wang, J. T.; Lee, W. S.; Huang, J. H.; McDonald, L. C.; Huang, I. W.; Chang, S. C.

In: European Journal of Clinical Microbiology and Infectious Diseases, Vol. 29, No. 12, 12.2010, p. 1553-1559.

Research output: Contribution to journalArticle

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