Cardiovascular pressor effects of orexins in the dorsomedial hypothalamus

Tzu Ling Li, Jennifer Y.S. Chen, Shang Cheng Huang, Yu Wen E. Dai, Ling Ling Hwang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Orexins are important regulators of cardiovascular functions in various physiological and pathological conditions. The dorsomedial hypothalamus (DMH), an essential mediator of cardiovascular responses to stress, contains dense orexinergic innervations and receptors. We examined whether orexins can regulate cardiovascular functions through their actions in the DMH in anesthetized rats. An intra-DMH injection of orexin A (30 pmol) produced elevation of arterial pressure and heart rate. Orexin A-sensitive sites were located within or immediately adjacent to the DMH and larger responses were induced at the compact part of the dorsomedial hypothalamic nucleus. Orexin A-induced responses were attenuated by intra-DMH pretreatment with an orexin receptor 1 (OX1R) antagonist, SB-334867 (15 nmol) (17.7 ± 2.8 vs. 5.2 ± 1.0 mmHg; 54.6 ± 10.0 vs. 22.8 ± 7.4 beats/min). Intra-DMH applied [Ala11,D-Leu15]-orexin B (300 pmol), an orexin receptor 2 (OX2R) agonist, elicited cardiovascular responses mimicking the responses of orexin A, except for a smaller pressor response (7.4 ± 1.7 vs. 16.4 ± 1.8 mmHg). In a series of experiment, effects of orexin B (100 pmol) and then orexin A (30 pmol), were examined at a same site. Two patterns of responses were observed in 12 intra-DMH sites: (1) both orexin A and B (9 sites), and (2) only orexin A (3 sites) induced cardiovascular responses, respectively suggesting OX1 R/OX2R-mediated and OX1R-predominant mechanisms. In conclusion, orexins regulated cardiovascular functions through OX1R/OX2R- or OX1R-mediated mechanisms at different locations in the DMH.

Original languageEnglish
Pages (from-to)343-350
Number of pages8
JournalEuropean Journal of Pharmacology
Volume818
DOIs
Publication statusPublished - Jan 5 2018

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Hypothalamus
Orexin Receptors
Orexins
Dorsomedial Hypothalamic Nucleus
Arterial Pressure
Heart Rate
Injections

Keywords

  • Blood pressure
  • Dorsomedial hypothalamus
  • Hypocretin
  • Orexin
  • SB334867
  • [Ala,D-Leu]-orexin B

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cardiovascular pressor effects of orexins in the dorsomedial hypothalamus. / Li, Tzu Ling; Chen, Jennifer Y.S.; Huang, Shang Cheng; Dai, Yu Wen E.; Hwang, Ling Ling.

In: European Journal of Pharmacology, Vol. 818, 05.01.2018, p. 343-350.

Research output: Contribution to journalArticle

Li, Tzu Ling ; Chen, Jennifer Y.S. ; Huang, Shang Cheng ; Dai, Yu Wen E. ; Hwang, Ling Ling. / Cardiovascular pressor effects of orexins in the dorsomedial hypothalamus. In: European Journal of Pharmacology. 2018 ; Vol. 818. pp. 343-350.
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