Cardiovascular effects of mastoparan B and its structural requirements

Ho Chewn-Lang Ho, Hwang Ling-Ling Hwang, Lin Yah-Luen Lin, Chen Chiung-Tong Chen, Yu Hui-Ming Yu, Wang Kung-Tsung Wang

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13 Citations (Scopus)

Abstract

Mastoparan B is a cationic, amphiphilic tetradecapeptide (LKLKSIVSWAKKVL-CONH2) isolated from the venom of the hornet Vespa basalis. Intravenous injection of the peptide into rats caused a profound depression of blood pressure and cardiac function, which was inhibited by cyproheptadine, reserpine and multiple doses of compound 48 80, but not by diphenhydramine and cromolyn. Mastoparan from Paravespula lewisii showed little cardiovascular inihibitory activity. A synthetic mastoparan B analog in which lysine at position 2 was replaced by asparagine showed a marked decrease in the cardiovascular depressor effects, while replacing lysine at position 4, 11 or 12 with leucine did not cause a significant reduction in these effects. Replacing lysine at position 12 with leucine even caused a more sustained depressor effect. However, the analog in which lysines at positions 11 and 12 were replaced by leucine lost its cardiovascular inhibitory activity. Replacing tryptophan at position 9 with phenylalanine in mastoparan B did not affect its activity. It is concluded that mastoparan B is involved in the cardiovascular disturbances induced by the hornet venom. Lysine at position 2 is a critical residue for the cardiovascular effects of mastoparan B. A peptide molecule with two lysine residues, one located close to the amino terminus and the other near the carboxyl end of the peptide, appears to be the optimal structure for eliciting the cardiovascular depressor effects.

Original languageEnglish
Pages (from-to)259-264
Number of pages6
JournalEuropean Journal of Pharmacology
Volume259
Issue number3
DOIs
Publication statusPublished - Jul 11 1994
Externally publishedYes

Keywords

  • Cardiovascular effect
  • Hornet (Vespa basalis) venom
  • Mastoparan B
  • Structural requirement

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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  • Cite this

    Chewn-Lang Ho, H., Ling-Ling Hwang, H., Yah-Luen Lin, L., Chiung-Tong Chen, C., Hui-Ming Yu, Y., & Kung-Tsung Wang, W. (1994). Cardiovascular effects of mastoparan B and its structural requirements. European Journal of Pharmacology, 259(3), 259-264. https://doi.org/10.1016/0014-2999(94)90652-1