Carboxylated carbon nanomaterials in cell cycle and apoptotic cell death regulation

Kuen Chan Lee, Pei Ying Lo, Guang Yu Lee, Jia Huei Zheng, Er Chieh Cho

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Carbon nanomaterials, include carbon nanotubes and graphene nanosheets, have drawn an increasing amount of attention because of their potential applications in daily life or in providing novel therapeutic possibilities for treating diseases. However, the overall biocompatibility, the potential toxic effects of carbon nanomaterials toward human cells, and their modulations in cellular mechanism, are not fully understood. Herein, four types of carbon nanomaterials, include long and short carbon nanotubes and graphene nanosheets, at low and high concentrations, were functionalized and dispersed in the biocompatible buffer for assessment. The surface structure, the morphology, and chemical composition of carbon nanomaterials were characterized. Also, biological assays investigating cellular viability, vitality, cell cycle, and apoptotic cell death were applied on cells co-incubated with nanomaterials, to evaluate the biocompatibility of these nanomaterials in human cells. Our data suggested that even though co-incubation of nanomaterials did not seem to affect the viability of cells notably, high concentrations (50 ug/ml) of SW could lead to unhealthy cells, and we observed dramatic G2 arrest effect mediated by p21 induction in high SW incubated cells. Other nanomaterials at high concentration may also alter cell cycle profile of the cells. In summary, our data demonstrated that these nanomaterials could regulate cell cycle and lead to apoptosis at high concentrations, and the underling molecular mechanisms have been addressed. Caution should be taken on their concentration when nanomaterials are in used in future medical applications.

Original languageEnglish
Pages (from-to)14-21
Number of pages8
JournalJournal of Biotechnology
Volume296
DOIs
Publication statusPublished - Apr 20 2019

Fingerprint

Nanostructures
Cell death
Nanostructured materials
Cell Cycle
Cell Death
Carbon
Cells
Carbon Nanotubes
Graphite
Nanosheets
Biocompatibility
Graphene
Carbon nanotubes
Poisons
Medical applications
Surface structure
Biological Assay
Assays
Cell Survival
Buffers

Keywords

  • Apoptosis
  • Biocompatibility
  • Carbon nanomaterials
  • Cell cycle
  • Toxicity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Carboxylated carbon nanomaterials in cell cycle and apoptotic cell death regulation. / Lee, Kuen Chan; Lo, Pei Ying; Lee, Guang Yu; Zheng, Jia Huei; Cho, Er Chieh.

In: Journal of Biotechnology, Vol. 296, 20.04.2019, p. 14-21.

Research output: Contribution to journalArticle

Lee, Kuen Chan ; Lo, Pei Ying ; Lee, Guang Yu ; Zheng, Jia Huei ; Cho, Er Chieh. / Carboxylated carbon nanomaterials in cell cycle and apoptotic cell death regulation. In: Journal of Biotechnology. 2019 ; Vol. 296. pp. 14-21.
@article{c78523bb4e5046f3b5d45f60fde40c6d,
title = "Carboxylated carbon nanomaterials in cell cycle and apoptotic cell death regulation",
abstract = "Carbon nanomaterials, include carbon nanotubes and graphene nanosheets, have drawn an increasing amount of attention because of their potential applications in daily life or in providing novel therapeutic possibilities for treating diseases. However, the overall biocompatibility, the potential toxic effects of carbon nanomaterials toward human cells, and their modulations in cellular mechanism, are not fully understood. Herein, four types of carbon nanomaterials, include long and short carbon nanotubes and graphene nanosheets, at low and high concentrations, were functionalized and dispersed in the biocompatible buffer for assessment. The surface structure, the morphology, and chemical composition of carbon nanomaterials were characterized. Also, biological assays investigating cellular viability, vitality, cell cycle, and apoptotic cell death were applied on cells co-incubated with nanomaterials, to evaluate the biocompatibility of these nanomaterials in human cells. Our data suggested that even though co-incubation of nanomaterials did not seem to affect the viability of cells notably, high concentrations (50 ug/ml) of SW could lead to unhealthy cells, and we observed dramatic G2 arrest effect mediated by p21 induction in high SW incubated cells. Other nanomaterials at high concentration may also alter cell cycle profile of the cells. In summary, our data demonstrated that these nanomaterials could regulate cell cycle and lead to apoptosis at high concentrations, and the underling molecular mechanisms have been addressed. Caution should be taken on their concentration when nanomaterials are in used in future medical applications.",
keywords = "Apoptosis, Biocompatibility, Carbon nanomaterials, Cell cycle, Toxicity",
author = "Lee, {Kuen Chan} and Lo, {Pei Ying} and Lee, {Guang Yu} and Zheng, {Jia Huei} and Cho, {Er Chieh}",
year = "2019",
month = "4",
day = "20",
doi = "10.1016/j.jbiotec.2019.02.005",
language = "English",
volume = "296",
pages = "14--21",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",

}

TY - JOUR

T1 - Carboxylated carbon nanomaterials in cell cycle and apoptotic cell death regulation

AU - Lee, Kuen Chan

AU - Lo, Pei Ying

AU - Lee, Guang Yu

AU - Zheng, Jia Huei

AU - Cho, Er Chieh

PY - 2019/4/20

Y1 - 2019/4/20

N2 - Carbon nanomaterials, include carbon nanotubes and graphene nanosheets, have drawn an increasing amount of attention because of their potential applications in daily life or in providing novel therapeutic possibilities for treating diseases. However, the overall biocompatibility, the potential toxic effects of carbon nanomaterials toward human cells, and their modulations in cellular mechanism, are not fully understood. Herein, four types of carbon nanomaterials, include long and short carbon nanotubes and graphene nanosheets, at low and high concentrations, were functionalized and dispersed in the biocompatible buffer for assessment. The surface structure, the morphology, and chemical composition of carbon nanomaterials were characterized. Also, biological assays investigating cellular viability, vitality, cell cycle, and apoptotic cell death were applied on cells co-incubated with nanomaterials, to evaluate the biocompatibility of these nanomaterials in human cells. Our data suggested that even though co-incubation of nanomaterials did not seem to affect the viability of cells notably, high concentrations (50 ug/ml) of SW could lead to unhealthy cells, and we observed dramatic G2 arrest effect mediated by p21 induction in high SW incubated cells. Other nanomaterials at high concentration may also alter cell cycle profile of the cells. In summary, our data demonstrated that these nanomaterials could regulate cell cycle and lead to apoptosis at high concentrations, and the underling molecular mechanisms have been addressed. Caution should be taken on their concentration when nanomaterials are in used in future medical applications.

AB - Carbon nanomaterials, include carbon nanotubes and graphene nanosheets, have drawn an increasing amount of attention because of their potential applications in daily life or in providing novel therapeutic possibilities for treating diseases. However, the overall biocompatibility, the potential toxic effects of carbon nanomaterials toward human cells, and their modulations in cellular mechanism, are not fully understood. Herein, four types of carbon nanomaterials, include long and short carbon nanotubes and graphene nanosheets, at low and high concentrations, were functionalized and dispersed in the biocompatible buffer for assessment. The surface structure, the morphology, and chemical composition of carbon nanomaterials were characterized. Also, biological assays investigating cellular viability, vitality, cell cycle, and apoptotic cell death were applied on cells co-incubated with nanomaterials, to evaluate the biocompatibility of these nanomaterials in human cells. Our data suggested that even though co-incubation of nanomaterials did not seem to affect the viability of cells notably, high concentrations (50 ug/ml) of SW could lead to unhealthy cells, and we observed dramatic G2 arrest effect mediated by p21 induction in high SW incubated cells. Other nanomaterials at high concentration may also alter cell cycle profile of the cells. In summary, our data demonstrated that these nanomaterials could regulate cell cycle and lead to apoptosis at high concentrations, and the underling molecular mechanisms have been addressed. Caution should be taken on their concentration when nanomaterials are in used in future medical applications.

KW - Apoptosis

KW - Biocompatibility

KW - Carbon nanomaterials

KW - Cell cycle

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=85063038396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063038396&partnerID=8YFLogxK

U2 - 10.1016/j.jbiotec.2019.02.005

DO - 10.1016/j.jbiotec.2019.02.005

M3 - Article

VL - 296

SP - 14

EP - 21

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

ER -