Carbonic anhydrase 2-like a and 15a are involved in acid-base regulation and Na+ uptake in zebrafish H+-ATPase-rich cells

Tzung Yi Lin, Bo Kai Liao, Jiun Lin Horng, Jia Jiun Yan, Chung Der Hsiao, Pung Pung Hwang

Research output: Contribution to journalArticle

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Abstract

H+-ATPase-rich (HR) cells in zebrafish gills/skin were found to carry out Na+ uptake and acid-base regulation through a mechanism similar to that which occurs in mammalian proximal tubular cells. However, the roles of carbonic anhydrases (CAs) in this mechanism in zebrafish HR cells are still unclear. The present study used a functional genomic approach to identify 20 CA isoforms in zebrafish. By screening with whole mount in situ hybridization, only zca2-like a and zca15a were found to be expressed in specific groups of cells in zebrafish gills/skin, and further analyses by triple in situ hybridization and immunocytochemistry demonstrated specific colocalizations of the two zca isoforms in HR cells. Knockdown of zca2-like a caused no change in and knockdown of zca15a caused an increase in H+ activity at the apical surface of HR cells at 24 h postfertilization (hpf). Later, at 96 hpf, both the zca2-like a and zca15a morphants showed decreased H+ activity and increased Na+ uptake, with concomitant upregulation of znhe3b and downregulation of zatp6v1a (H+-ATPase A-subunit) expressions. Acclimation to both acidic and low-Na+ fresh water caused upregulation of zca15a expression but did not change the zca2-like a mRNA level in zebrafish gills. These results provide molecular physiological evidence to support the roles of these two zCA isoforms in Na+ uptake and acid-base regulation mechanisms in zebrafish HR cells.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume294
Issue number5
DOIs
Publication statusPublished - May 2008
Externally publishedYes

Fingerprint

Proton-Translocating ATPases
Carbonic Anhydrases
Zebrafish
Acids
Protein Isoforms
Skin
In Situ Hybridization
Up-Regulation
Acclimatization
Fresh Water
Screening
Down-Regulation
Immunohistochemistry
Messenger RNA
Water

Keywords

  • Embryo
  • Gill
  • Ionocytes
  • Na/H exchanger
  • Skin

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Clinical Biochemistry

Cite this

Carbonic anhydrase 2-like a and 15a are involved in acid-base regulation and Na+ uptake in zebrafish H+-ATPase-rich cells. / Lin, Tzung Yi; Liao, Bo Kai; Horng, Jiun Lin; Yan, Jia Jiun; Hsiao, Chung Der; Hwang, Pung Pung.

In: American Journal of Physiology - Cell Physiology, Vol. 294, No. 5, 05.2008.

Research output: Contribution to journalArticle

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