Carbon monoxide ameliorates Staphylococcus aureus-elicited COX-2/IL-6/MMP-9-dependent human aortic endothelial cell migration and inflammatory responses

Ming Horng Tsai, Chuen Mao Yang, Kuo Ting Chang, Chu Chun Chuang, Wei Ning Lin, Rong San Jiang, Cheng Hsun Wu, I. Ta Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Staphylococcus aureus (S. aureus) can often lead to many life-threatening diseases. It has the ability to invade normal endovascular tissue. Acute inflammation and its resolution are important to ensure bacterial clearance and limit tissue injury. Carbon monoxide (CO) has been shown to exert anti-inflammatory effects in various tissues and organ systems. In our study, we investigated the effects and the mechanisms of carbon monoxide releasing molecule-2 (CORM-2) on S. aureus-induced inflammatory responses in human aortic endothelial cells (HAECs). We proved that S. aureus induced cyclooxygenase-2 (COX-2)/prostaglandin E 2 (PGE 2 )/interleukin-6 (IL-6)/matrix metallopeptidase-9 (MMP-9) expression and cell migration, which were decreased by CORM-2. Moreover, CORM-2 had no effects on TLR2 mRNA levels in response to S. aureus. Interestingly, we proved that S. aureus decreased intracellular ROS generation, suggesting that the inhibition of ROS further promoted inflammatory responses. However, CORM-2 significantly inhibited S. aureus-induced inflammation by increasing intracellular ROS generation. S. aureus-induced NF-κB activation was also inhibited by CORM-2. Finally, we proved that S. aureus induced levels of the biomarkers of inflammation in cardiovascular diseases, which were inhibited by CORM-2. Taken together, these results suggest that CORM-2 inhibits S. aureus-induced COX-2/PGE 2 /IL-6/MMP-9 expression and aorta inflammatory responses by increasing the ROS generation and reducing the inflammatory molecules levels.

Original languageEnglish
Pages (from-to)40-49
Number of pages10
JournalImmunology Letters
Volume203
DOIs
Publication statusPublished - Nov 1 2018
Externally publishedYes

Fingerprint

Metalloproteases
Cyclooxygenase 2
Carbon Monoxide
Cell Movement
Staphylococcus aureus
Interleukin-6
Endothelial Cells
Prostaglandins E
Inflammation
Aorta
Anti-Inflammatory Agents
Cardiovascular Diseases
Biomarkers
Messenger RNA
Wounds and Injuries

Keywords

  • Carbon monoxide
  • Cyclooxygenase-2
  • Interleukin-6
  • Matrix metallopeptidase-9
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Carbon monoxide ameliorates Staphylococcus aureus-elicited COX-2/IL-6/MMP-9-dependent human aortic endothelial cell migration and inflammatory responses. / Tsai, Ming Horng; Yang, Chuen Mao; Chang, Kuo Ting; Chuang, Chu Chun; Lin, Wei Ning; Jiang, Rong San; Wu, Cheng Hsun; Lee, I. Ta.

In: Immunology Letters, Vol. 203, 01.11.2018, p. 40-49.

Research output: Contribution to journalArticle

Tsai, Ming Horng ; Yang, Chuen Mao ; Chang, Kuo Ting ; Chuang, Chu Chun ; Lin, Wei Ning ; Jiang, Rong San ; Wu, Cheng Hsun ; Lee, I. Ta. / Carbon monoxide ameliorates Staphylococcus aureus-elicited COX-2/IL-6/MMP-9-dependent human aortic endothelial cell migration and inflammatory responses. In: Immunology Letters. 2018 ; Vol. 203. pp. 40-49.
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