Carbon dots prepared from ginger exhibiting efficient inhibition of human hepatocellular carcinoma cells

Chi Lin Li, Chung Mao Ou, Chih Ching Huang, Wei Cheng Wu, Yi Ping Chen, Tzu En Lin, Lin Chen Ho, Chia Wei Wang, Chung Chien Shih, Hang Cheng Zhou, Ying Chu Lee, Woan Fang Tzeng, Tzeon Jye Chiou, Sin Tak Chu, Jinshun Cang, Huan Tsung Chang

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Fluorescent carbon nanodots (C-dots; 4.3 ± 0.8 nm) from fresh tender ginger juice provide high suppression of the growth of human hepatocellular carcinoma cells (HepG2), with low toxicity to normal mammary epithelial cells (MCF-10A) and normal liver cells (FL83B). The inhibition is selective to HepG2 over other tested cancer cells, including human lung cancer cell line (A549), human breast cancer cell line (MDA-MB-231), and human cervical cancer cell line (HeLa). Western blot results reveal that the C-dots up-regulate the expression of p53 protein only in the HepG2 cell line. The 50% inhibiting concentration (IC50) value of the C-dots on HepG2 cells is 0.35 mg mL-1. Image cytometry results show significant uptake of C-dots by HepG2 cells that induce intracellular production of reactive oxygen species (ROS, 18.2-fold increased), while other cells remain almost the same in ROS levels after treatment with C-dots (1.11 mg mL-1). The C-dots trigger the pro-apoptotic factor to promote HepG2 cell apoptosis. The C-dots effectively inhibit the growth of tumors in nude mice (104 ± 14 vs. 3.7 ± 0.2 mg with and without treatment within 14 days). This journal is

Original languageEnglish
Pages (from-to)4564-4571
Number of pages8
JournalJournal of Materials Chemistry B
Volume2
Issue number28
DOIs
Publication statusPublished - Jul 28 2014
Externally publishedYes

Fingerprint

Ginger
Hep G2 Cells
Hepatocellular Carcinoma
Carbon
Cells
Cell Line
Image Cytometry
Growth
Nude Mice
Uterine Cervical Neoplasms
Inhibitory Concentration 50
Cell death
Reactive Oxygen Species
Lung Neoplasms
Neoplasms
Breast
Up-Regulation
Liver
Western Blotting
Epithelial Cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Medicine(all)
  • Materials Science(all)

Cite this

Carbon dots prepared from ginger exhibiting efficient inhibition of human hepatocellular carcinoma cells. / Li, Chi Lin; Ou, Chung Mao; Huang, Chih Ching; Wu, Wei Cheng; Chen, Yi Ping; Lin, Tzu En; Ho, Lin Chen; Wang, Chia Wei; Shih, Chung Chien; Zhou, Hang Cheng; Lee, Ying Chu; Tzeng, Woan Fang; Chiou, Tzeon Jye; Chu, Sin Tak; Cang, Jinshun; Chang, Huan Tsung.

In: Journal of Materials Chemistry B, Vol. 2, No. 28, 28.07.2014, p. 4564-4571.

Research output: Contribution to journalArticle

Li, CL, Ou, CM, Huang, CC, Wu, WC, Chen, YP, Lin, TE, Ho, LC, Wang, CW, Shih, CC, Zhou, HC, Lee, YC, Tzeng, WF, Chiou, TJ, Chu, ST, Cang, J & Chang, HT 2014, 'Carbon dots prepared from ginger exhibiting efficient inhibition of human hepatocellular carcinoma cells', Journal of Materials Chemistry B, vol. 2, no. 28, pp. 4564-4571. https://doi.org/10.1039/c4tb00216d
Li, Chi Lin ; Ou, Chung Mao ; Huang, Chih Ching ; Wu, Wei Cheng ; Chen, Yi Ping ; Lin, Tzu En ; Ho, Lin Chen ; Wang, Chia Wei ; Shih, Chung Chien ; Zhou, Hang Cheng ; Lee, Ying Chu ; Tzeng, Woan Fang ; Chiou, Tzeon Jye ; Chu, Sin Tak ; Cang, Jinshun ; Chang, Huan Tsung. / Carbon dots prepared from ginger exhibiting efficient inhibition of human hepatocellular carcinoma cells. In: Journal of Materials Chemistry B. 2014 ; Vol. 2, No. 28. pp. 4564-4571.
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