Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes

Hsin Ying Wu, An Chi Chang, Chia Chi Wang, Fu Hua Kuo, Chi Ya Lee, Der Zen Liu, Tong Rong Jan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

It has been documented that cannabidiol (CBD) induced apoptosis in a variety of transformed cells, including lymphocytic and monocytic leukemias. In contrast, a differential sensitivity between normal lymphocytes and monocytes to CBD-mediated apoptosis has been reported. The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72h. CBD markedly enhanced apoptosis of freshly isolated monocytes in a time- and concentration-dependent manner, whereas precultured monocytes were insensitive. By comparison, both cells were sensitive to doxorubicin-induced apoptosis. CBD significantly diminished the cellular thiols and glutathione in freshly isolated monocytes. The apoptosis induced by CBD was abrogated in the presence of N-acetyl-L-cysteine, a precursor of glutathione. In addition, precultured monocytes contained a significantly greater level of glutathione and heme oxygenase-1 (HO-1) compared to the freshly isolated cells. The HO-1 competitive inhibitor zinc protoporphyrin partially but significantly restored the sensitivity of precultured monocytes to CBD-mediated apoptosis. Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournalToxicology and Applied Pharmacology
Volume246
Issue number3
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Apoptosis
  • Cannabidiol
  • Glutathione
  • Heme oxygenase-1
  • Monocyte
  • Thiols

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

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