cAMP response element-binding protein activation in ligation preconditioning in neonatal brain

Hsueh Te Lee, Ying Chao Chang, Lin Yu Wang, Shan Tair Wang, Chao Ching Huang, Chien Jung Ho

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Perinatal hypoxic-ischemic (HI) brain injury is a major cause of permanent neurological dysfunction in children. An approach to study the treatment of neonatal HI encephalopathy that allows for neuroprotection is to investigate the states of tolerance to HI. Twenty-four-hour carotid-artery ligation preconditioning established by delaying the onset of hypoxia for 24 hours after permanent unilateral carotid ligation rats markedly diminished the cerebral injury, however, the signaling mechanisms of this carotid-artery ligation preconditioning in neonatal rats remain unknown. Ligation of the carotid artery 24 hours before hypoxia provided complete neuroprotection and produced improved performance on the Morris water maze compared with ligation performed 1 hour before hypoxia. Carotid artery ligation 6 hours before hypoxia produced intermediate benefit. The 24-hour carotid-artery ligation preconditioning was associated with a robust and sustained activation of a transcription factor, the cAMP response element-binding protein (CREB), on its phosphorylation site on Ser133. Intracerebroventricular infusions of antisense CREB oligodeoxynucleotides significantly reduced the 24-hour carotid-artery ligation-induced neuroprotection effects by decreasing CREB expressions. Pharmacological activation of the cAMP-CREB signaling with rolipram 24 hours before hypoxia protected rat pups at behavioral and pathological levels by sustained increased CREB phosphorylation. This study suggests that 24-hour carotid-artery ligation preconditioning provides important mechanisms for potential pharmacological preconditioning against neonatal HI brain injury.

Original languageEnglish
Pages (from-to)611-623
Number of pages13
JournalAnnals of Neurology
Volume56
Issue number5
DOIs
Publication statusPublished - Nov 2004
Externally publishedYes

Fingerprint

Cyclic AMP Response Element-Binding Protein
Ligation
Carotid Arteries
Brain
Brain Injuries
Phosphorylation
Pharmacology
Rolipram
Intraventricular Infusions
Brain Hypoxia-Ischemia
Oligodeoxyribonucleotides
Transcription Factors
Hypoxia
Water

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

cAMP response element-binding protein activation in ligation preconditioning in neonatal brain. / Lee, Hsueh Te; Chang, Ying Chao; Wang, Lin Yu; Wang, Shan Tair; Huang, Chao Ching; Ho, Chien Jung.

In: Annals of Neurology, Vol. 56, No. 5, 11.2004, p. 611-623.

Research output: Contribution to journalArticle

Lee, Hsueh Te ; Chang, Ying Chao ; Wang, Lin Yu ; Wang, Shan Tair ; Huang, Chao Ching ; Ho, Chien Jung. / cAMP response element-binding protein activation in ligation preconditioning in neonatal brain. In: Annals of Neurology. 2004 ; Vol. 56, No. 5. pp. 611-623.
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