Calmodulin-dependent protein kinase gamma 3 (CamKIIγ3) mediates the cell cycle resumption of metaphase II eggs in mouse

Heng Yu Chang, Kyra Minahan, Julie A. Merriman, Keith T. Jones

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Mature mammalian eggs are ovulated arrested at meiotic metaphase II. Sperm break this arrest by an oscillatory Ca2+ signal that is necessary and sufficient for the two immediate events of egg activation: cell cycle resumption and cortical granule release. Previous work has suggested that cell cycle resumption, but not cortical granule release, is mediated by calmodulin-dependent protein kinase II (CamKII). Here we find that mouse eggs contain detectable levels of only one CamKII isoform, gamma 3. Antisense morpholino knockdown of CamKIIγ3 during oocyte maturation produces metaphase II eggs that are insensitive to parthenogenetic activation by Ca2+ stimulation and insemination. The effect is specific to this morpholino, as a 5-base-mismatch morpholino is without effect, and is rescued by CamKIIγ3 or constitutively active CamKII cRNAs. Although CamKII-morpholino-treated eggs fail to exit metaphase II arrest, cortical granule exocytosis is not blocked. Therefore, CamKIIγ3 plays a necessary and sufficient role in transducing the oscillatory Ca2+ signal into cell cycle resumption, but not into cortical granule release.

Original languageEnglish
Pages (from-to)4077-4081
Number of pages5
JournalDevelopment
Volume136
Issue number24
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 2
Morpholinos
Calcium-Calmodulin-Dependent Protein Kinases
Metaphase
Eggs
Cell Cycle
Complementary RNA
Insemination
Exocytosis
Oocytes
Ovum
Spermatozoa
Protein Isoforms

Keywords

  • Calcium
  • CamKII (Camk2)
  • Cell cycle
  • Fertilization
  • Mouse

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Calmodulin-dependent protein kinase gamma 3 (CamKIIγ3) mediates the cell cycle resumption of metaphase II eggs in mouse. / Chang, Heng Yu; Minahan, Kyra; Merriman, Julie A.; Jones, Keith T.

In: Development, Vol. 136, No. 24, 2009, p. 4077-4081.

Research output: Contribution to journalArticle

Chang, Heng Yu ; Minahan, Kyra ; Merriman, Julie A. ; Jones, Keith T. / Calmodulin-dependent protein kinase gamma 3 (CamKIIγ3) mediates the cell cycle resumption of metaphase II eggs in mouse. In: Development. 2009 ; Vol. 136, No. 24. pp. 4077-4081.
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