Calibration of glucose oxidase-based test strips for capillary blood measurement with oxygen saturated venous blood samples

Wen Jing Cheng, Chii Wann Lin, Tai Guang Wu, Chein Shyong Su, Ming Song Hsieh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Glucose oxidase biosensors are used in self-monitoring blood glucose concentrations. The capillary blood glucose quantitation requires a calibration curve. Due to the limitation in obtaining calibration curve from capillary blood, an alternate approach by using venous blood for neonatal measurement was investigated. Methods: A signal correlation between oxygen saturated venous blood and capillary blood was derived. The hematocrit effect was studied for different glucose concentrations. The calibrated glucose strips were validated by neonatal intensive care unit (NICU) samples. Results: A simple equation, finger blood signal=1.39 * (oxygen saturated venous blood signal) - 31.2 was derived. The rate of change in glucose concentration due to hematocrit effect was low in lower glucose concentration samples. The BeneCheck Glucose Strips were compared with Beckman Coulter analyzer by using 52 NICU samples. More than 95% of test results were within the variation of ± 10 mg/dl of bias and ± 15% of bias% when glucose concentration is

Original languageEnglish
Pages (from-to)152-157
Number of pages6
JournalClinica Chimica Acta
Volume415
DOIs
Publication statusPublished - Jan 16 2013

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Keywords

  • Capillary blood
  • Electrochemical
  • Glucose oxidase biosensor
  • Glucose strip
  • Hematocrit
  • Neonatal

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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