Calcium-activated gene transfection from DNA/poly(Amic acid-co-imide) complexes

Szu-Yuan Wu, Li Ting Chang, Sydeny Peng, Hsieh Chih Tsai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, we synthesized a water-soluble poly(amic acid-co-imide) (PA-I) from ethylenediaminetetraacetic dianhydride (EDTA) and 2,2′-(ethylenedioxy)bis(ethylamine) that possesses comparable transfection efficiency to that of polyethylenimine (PEI), when prepared in combination with divalent calcium cations. The polycondensation of monomers afforded poly(amic acid) (PA) precursors, and subsequent thermal imidization resulted in the formation of PA-I. At a polymer/DNA ratio (indicated by the molar ratio of nitrogen in the polymer to phosphate in DNA) of 40, complete retardation of the DNA band was observed by gel electrophoresis, indicating the strong association of DNA with PA-I. A zeta potential of-22 mV was recorded for the PA-I polymer solution, and no apparent cytotoxicity was observed at concentrations up to 500 μg⋅mL-1. In the presence of divalent Ca2+, the transfection efficiency of PA-I was higher than that of PA, due to the formation of a copolymer/Ca2+/DNA polyplex and the reduction in negative charge due to thermal cyclization. Interestingly, a synergistic effect of Ca2+and the synthesized copolymer on DNA transfection was observed. The use of Ca2+or copolymer alone resulted in unsatisfactory delivery, whereas the formation of three-component polyplexes synergistically increased DNA transfection. Our findings demonstrated that a PA-I/Ca2+/DNA polyplex could serve as a promising candidate for gene delivery.

Original languageEnglish
Pages (from-to)1637-1647
Number of pages11
JournalInternational Journal of Nanomedicine
Volume10
DOIs
Publication statusPublished - Feb 27 2015

Fingerprint

Imides
Transfection
Calcium
DNA
Genes
Acids
Polymers
Copolymers
Hot Temperature
Polyethyleneimine
Cyclization
Divalent Cations
Zeta potential
Polymer solutions
Cytotoxicity
Polycondensation
Electrophoresis
Cations
Phosphates
Nitrogen

Keywords

  • Gene carrier
  • Poly(amic acid)
  • Polyimide

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Calcium-activated gene transfection from DNA/poly(Amic acid-co-imide) complexes. / Wu, Szu-Yuan; Chang, Li Ting; Peng, Sydeny; Tsai, Hsieh Chih.

In: International Journal of Nanomedicine, Vol. 10, 27.02.2015, p. 1637-1647.

Research output: Contribution to journalArticle

Wu, Szu-Yuan ; Chang, Li Ting ; Peng, Sydeny ; Tsai, Hsieh Chih. / Calcium-activated gene transfection from DNA/poly(Amic acid-co-imide) complexes. In: International Journal of Nanomedicine. 2015 ; Vol. 10. pp. 1637-1647.
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