Caffeic acid phenethyl ester upregulates N-myc downstream regulated gene 1 via ERK pathway to inhibit human oral cancer cell growth in vitro and in vivo

Li Chuan Chung, Kun Chun Chiang, Tsui Hsia Feng, Kang Shuo Chang, Sung Ting Chuang, Yu Jen Chen, Ke Hung Tsui, Jehn Chuan Lee, Horng Heng Juang

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Scope: Caffeic acid phenethyl ester (CAPE), a bioactive component of propolis, is considered as a new anti-cancer agent. Oral squamous cell carcinoma (OSCC) is the most common oral cancer with unsatisfying survival. N-myc downstream regulated family genes (NDRGs) involve in numerous physiological processes. We investigated the anti-cancer effect of CAPE on OSCC and related mechanisms. Methods and results: Cell proliferation assay, western blot, gene transfection and knockdown, and reporter assay were applied. We showed that CAPE attenuated OSCC cell proliferation and invasion in vitro, and safely and effectively inhibited OSCC cell growth in a xenograft animal model. CAPE treatment induced NDRG1, but not NDRG2 and NDRG3, expression in OSCC cells as determined by western blot, RT-qPCR, and reporter assay. The 5′-deletion assay demonstrated that CAPE increased NDRG1 promoter activity depending on the region of -128 to +46 of the 5′-flanking of NDRG1 gene. NDRG1 gene knockdown attenuated CAPE anti-growth effect on OSCC cells. CAPE activated mitogen-activated protein kinase (MAPK) signaling pathway. The extracellular signal regulated kinase (ERK) inhibitor (PD0325901) and ERK1 knockdown blocked CAPE-induced NDRG1 expression in OSCC cells. Conclusion: CAPE activated MAPK signaling pathway and increased NDRG1 expression through phosphorylation of ERK1/2 to repress OSCC cells growth.

Original languageEnglish
Article number1600842
JournalMolecular Nutrition and Food Research
Volume61
Issue number9
DOIs
Publication statusPublished - Sep 2017
Externally publishedYes

Keywords

  • CAPE
  • MAPK
  • NDRG1
  • OECM-1
  • Oral cancer
  • SAS

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

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