Caffeic acid phenethyl ester inhibits nuclear factor-κB and protein kinase B signalling pathways and induces caspase-3 expression in primary human CD4+ T cells

L. C. Wang, K. H. Chu, Y. C. Liang, Y. L. Lin, B. L. Chiang

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Summary Caffeic acid phenethyl ester (CAPE), an active component in propolis, is known to have anti-tumour, anti-inflammatory and anti-oxidant properties. In this study, the effects of CAPE on the functions of primary human CD4+ T cells were evaluated in vitro. CAPE significantly suppressed interferon (IFN)-γ and interleukin (IL)-5 production and proliferation of CD4+ T cells stimulated by soluble anti-CD3 and anti-CD28 monoclonal antibodies in both healthy subjects and asthmatic patients. CAPE inhibited nuclear factor (NF)-κB activation and protein kinase B (Akt) phosphorylation, but not p38 mitogen-activated protein kinase (MAPK) phosphorylation in T cells. CAPE also induced active caspase-3 expression in CD4+ T cells; CCR4+CD4+ T cells were more sensitive to CAPE induction than CXCR3+CD4+ T cells. Together, these results indicate that CAPE inhibits cytokine production and proliferation of T cells, which might be related to the NF-κB and Akt signalling pathways, and that CCR4+CD4+ T cells are more sensitive to CAPE inhibition. This study provides a new insight into the mechanisms of CAPE for immune regulation and a rationale for the use of propolis for the treatment of allergic disorders.

Original languageEnglish
Pages (from-to)223-232
Number of pages10
JournalClinical and Experimental Immunology
Volume160
Issue number2
DOIs
Publication statusPublished - May 2010

Keywords

  • Akt
  • CD4 T cells
  • Caffeic acid phenethyl ester
  • Caspase-3
  • NF-κB

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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