Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells

Wen Rui Hao, Li Chin Sung, Chun Chao Chen, Po Yuan Chen, Tzu Hurng Cheng, Hung Hsing Chao, Ju Chi Liu, Jin Jer Chen

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Moderate coffee consumption is inversely associated with cardiovascular disease mortality; however, mechanisms underlying this causal effect remain unclear. Cafestol, a diterpene found in coffee, has various properties, including an anti-inflammatory property. This study investigated the effect of cafestol on cyclic-strain-induced inflammatory molecule secretion in vascular endothelial cells. Cells were cultured under static or cyclic strain conditions, and the secretion of inflammatory molecules was determined using enzyme-linked immunosorbent assay. The effects of cafestol on mitogen-activated protein kinases (MAPK), heme oxygenase-1 (HO-1), and sirtuin 1 (Sirt1) signaling pathways were examined using Western blotting and specific inhibitors. Cafestol attenuated cyclic-strain-stimulated intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein- (MCP-) 1, and interleukin- (IL-) 8 secretion. Cafestol inhibited the cyclic-strain-induced phosphorylation of extracellular signal-regulated kinase and p38 MAPK. By contrast, cafestol upregulated cyclic-strain-induced HO-1 and Sirt1 expression. The addition of zinc protoporphyrin IX, sirtinol, or Sirt1 silencing (transfected with Sirt1 siRNA) significantly attenuated cafestol-mediated modulatory effects on cyclic-strain-stimulated ICAM-1, MCP-1, and IL-8 secretion. This is the first study to report that cafestol inhibited cyclic-strain-induced inflammatory molecule secretion, possibly through the activation of HO-1 and Sirt1 in endothelial cells. The results provide valuable insights into molecular pathways that may contribute to the effects of cafestol.

Original languageEnglish
Article number7861518
Number of pages1
JournalOxidative Medicine and Cellular Longevity
Volume2018
DOIs
Publication statusPublished - Jan 1 2018

Keywords

  • Chemokine CCL2/metabolism
  • Diterpenes/pharmacology
  • Extracellular Signal-Regulated MAP Kinases/metabolism
  • Heme Oxygenase-1/genetics
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Intercellular Adhesion Molecule-1/metabolism
  • Interleukin-8/metabolism
  • Phosphorylation/drug effects
  • RNA Interference
  • RNA, Small Interfering/metabolism
  • Reactive Oxygen Species/metabolism
  • Sirtuin 1/antagonists & inhibitors
  • Stress, Physiological
  • Up-Regulation/drug effects
  • p38 Mitogen-Activated Protein Kinases/metabolism

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Cell Biology

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