Cafestol, a coffee diterpene, inhibits urotensin II-induced interleukin-8 expression in human umbilical vein endothelial cells

Yi Ting Tsai, Li Chin Sung, Wen Ray Haw, Chun Chao Chen, Shu Fu Huang, Ju Chi Liu, Tzu Hurng Cheng, Po Yuan Chen, Shih Hurng Loh, Chien Sung Tsai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cafestol, a diterpene molecule found in the berries of Coffea arabica L. (Rubiaceae), has been shown to exercise anti-angiogenic and anti-tumorigenic effects. However, cafestol's cellular mechanism has yet to be fully investigated. We previously demonstrated that urotensin II enhanced interleukin-8 secretion by endothelial cells, thereby increasing endothelial cell proliferation. Urotensin II may also participate in angiogenesis and tumor infiltration by macrophages. However, the effects of cafestol on urotensin II-induced interleukin-8 expression and cellular proliferation have not been determined. Here, we showed that pretreatment with cafestol inhibited urotensin II-stimulated endothelial cell proliferation. Further experiments demonstrated that cafestol increased translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and expression of enhanced heme oxygenase-1. Moreover, cafestol inhibited expression of urotensin II-induced interleukin-8. Cafestol's inhibitory effects on interleukin-8 expression and cellular proliferation induced by urotensin II were significantly abrogated by heme oxygenase-1 silencing, suggesting it may be involved in mediating the effects of cafestol. This study reports that cafestol inhibits urotensin II-induced interleukin-8 expression and cell proliferation via Nrf2/heme oxygenase-1-dependent mechanism in endothelial cells. These findings provide novel insight into the signaling pathways that may be important in mediating the effects of cafestol.

Original languageEnglish
Pages (from-to)106-112
Number of pages7
JournalEuropean Journal of Pharmacology
Volume820
DOIs
Publication statusPublished - Feb 5 2018

Keywords

  • Cafestol
  • Endothelial cells
  • Heme oxygenase-1
  • Nuclear factor erythroid 2-related factor 2
  • Urotensin II, interleukin-8

ASJC Scopus subject areas

  • Pharmacology

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