CACC box and enhancer response of the human embryonic ε globin promoter

Kouros Motamed, Carol Bastiani, Zhang Qingyi, Arnold Bailey, C. K.James Shen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The functional interaction between the human ε globin promoter and an erythroid-specific transcription enhancer, 5′ HS-2, has been analyzed by transient expression assay. While stepwise deletion of DNA sequences between -852 and -122 had only small effects, removal of the CACC box at position -111 greatly decreased ε-globin promoter activity, as well as its response to the enhancer function of 5′ HS-2 in erythroid cells. Our data demonstrated that the three ubiquitous promoter elements, the CACC, CCAAT, and TATA boxes, of the ε-globin-encoding gene together form a minimal promoter that would interact efficiently with 5′ HS-2, and that at least the CACC box is an essential functional component of this enhancer-promoter interaction.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalGene
Volume123
Issue number2
DOIs
Publication statusPublished - Jan 30 1993
Externally publishedYes

Keywords

  • CCAAT box
  • erythroid cells
  • Recombinant DNA
  • TATA box
  • transcription factor
  • transient expression assay

ASJC Scopus subject areas

  • Genetics

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