C-terminus of Hsc70-interacting protein (CHIP) enhances stemness properties of human Wharton’s jelly mesenchymal stem cell

C. K. Yang, C. C. Feng, J. F. Lo, J. W. Chen, V. V. Padma, C. H. Lai, T. S. Chen, R. J. Chen, P. H. Liao, C. Y. Huang

Research output: Contribution to journalArticle

Abstract

Mesenchymal stem cells are an attractive source of multipotent cells in part because they are easy to obtain. Several E3 ligases regulate the stability and functions of various factors in different adult stem cells through the ubiquitylation pathway. We investigated the C-terminus of Hsc70-interacting protein (CHIP) E3 ligase that regulates pluripotency of human Wharton’s jelly mesenchymal stem cells (hWJMSC). We found that CHIP increases protein kinase B (Akt) phosphorylation by decreased expression of phosphatase and tensin homolog (PTEN), which suggests improvement of the survival pathway by CHIP over-expression. We also found that increased CHIP expression induced Sox2 and NANOG, which can promote stem cell self-renewal and prevent oxidative stress-induced senescence of hWJMSC by decreased p21. We found that CHIP could be used to enhance the multiple functions of hWJMSC.

Original languageEnglish
Pages (from-to)632-639
Number of pages8
JournalBiotechnic and Histochemistry
Volume93
Issue number8
DOIs
Publication statusPublished - Nov 17 2018

Keywords

  • Hsc70-interacting protein
  • human
  • mesenchymal stem cell
  • phosphatase and tensin homolog (PTEN)
  • protein kinase B (Akt)
  • self-renewal
  • Wharton’s jelly

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

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    Yang, C. K., Feng, C. C., Lo, J. F., Chen, J. W., Padma, V. V., Lai, C. H., Chen, T. S., Chen, R. J., Liao, P. H., & Huang, C. Y. (2018). C-terminus of Hsc70-interacting protein (CHIP) enhances stemness properties of human Wharton’s jelly mesenchymal stem cell. Biotechnic and Histochemistry, 93(8), 632-639. https://doi.org/10.1080/10520295.2018.1521990