Abstract
Characterization of nonacute retrovirus-induced neoplasms have greatly facilitated our understanding of the mechanisms by which host protooncogenes are activated. Here we report the molecular characterization of a newly identified chicken lymphoma, which does not involve the bursa of Fabricius. This lymphoma, specifically induced by reticuloendotheliosis virus (REV), is similar in tumor location and T-cell origin to Marek's disease, a herpesvirus-induced T-lymphoma. We show the c-myc is the specific target locus for REV insertion. Integrated proviruses are all located upstream of the c-myc coding exons, and 60% are 5' to the first noncoding exon. One-half of the proviruses are oriented in the opposite transcriptional direction as the c-myc gene. This insertion pattern is in contrast to the pattern in B-lymphomas induced by the same virus. While some of the proviruses in these T-lymphomas used the 3' LTR promoter to transcribe the downstream c-myc gene, others apparently activated a common, cryptic promoter located in the first intron. To our knowledge, this is the first molecular description of an avian T-lymphoma induced by a nonacute retrovirus.
| Original language | English |
|---|---|
| Pages (from-to) | 81-94 |
| Number of pages | 14 |
| Journal | Oncogene Research |
| Volume | 2 |
| Issue number | 1 |
| Publication status | Published - Dec 1 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
Cite this
C-myc activation in an unusual retrovirus-induced avian T-lymphoma resembling Marek's disease : Proviral insertion 5' of exon one enhances the expression of an intron promoter. / Isfort, R.; Witter, R. L.; Kung, H. J.
In: Oncogene Research, Vol. 2, No. 1, 01.12.1987, p. 81-94.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - C-myc activation in an unusual retrovirus-induced avian T-lymphoma resembling Marek's disease
T2 - Proviral insertion 5' of exon one enhances the expression of an intron promoter
AU - Isfort, R.
AU - Witter, R. L.
AU - Kung, H. J.
PY - 1987/12/1
Y1 - 1987/12/1
N2 - Characterization of nonacute retrovirus-induced neoplasms have greatly facilitated our understanding of the mechanisms by which host protooncogenes are activated. Here we report the molecular characterization of a newly identified chicken lymphoma, which does not involve the bursa of Fabricius. This lymphoma, specifically induced by reticuloendotheliosis virus (REV), is similar in tumor location and T-cell origin to Marek's disease, a herpesvirus-induced T-lymphoma. We show the c-myc is the specific target locus for REV insertion. Integrated proviruses are all located upstream of the c-myc coding exons, and 60% are 5' to the first noncoding exon. One-half of the proviruses are oriented in the opposite transcriptional direction as the c-myc gene. This insertion pattern is in contrast to the pattern in B-lymphomas induced by the same virus. While some of the proviruses in these T-lymphomas used the 3' LTR promoter to transcribe the downstream c-myc gene, others apparently activated a common, cryptic promoter located in the first intron. To our knowledge, this is the first molecular description of an avian T-lymphoma induced by a nonacute retrovirus.
AB - Characterization of nonacute retrovirus-induced neoplasms have greatly facilitated our understanding of the mechanisms by which host protooncogenes are activated. Here we report the molecular characterization of a newly identified chicken lymphoma, which does not involve the bursa of Fabricius. This lymphoma, specifically induced by reticuloendotheliosis virus (REV), is similar in tumor location and T-cell origin to Marek's disease, a herpesvirus-induced T-lymphoma. We show the c-myc is the specific target locus for REV insertion. Integrated proviruses are all located upstream of the c-myc coding exons, and 60% are 5' to the first noncoding exon. One-half of the proviruses are oriented in the opposite transcriptional direction as the c-myc gene. This insertion pattern is in contrast to the pattern in B-lymphomas induced by the same virus. While some of the proviruses in these T-lymphomas used the 3' LTR promoter to transcribe the downstream c-myc gene, others apparently activated a common, cryptic promoter located in the first intron. To our knowledge, this is the first molecular description of an avian T-lymphoma induced by a nonacute retrovirus.
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M3 - Article
C2 - 3505666
AN - SCOPUS:0023614889
VL - 2
SP - 81
EP - 94
JO - Oncogene Research
JF - Oncogene Research
SN - 0890-6467
IS - 1
ER -