C 60 fullerene nanoparticle prevents β-amyloid peptide induced cytotoxicity in neuro 2A cells

Tan Yi Lu, Pai Feng Kao, Chi-Ming Lee, Sheng Tung Huang, Chun Mao Lin

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7 Citations (Scopus)

Abstract

Oxidative stress, which is an early determinant of Alzheimer's disease (AD), is involved in mediating neuronal apoptosis in Aβ-induced cell death. C 60 fullerenes are known to behave like a "radical sponge" as they can sponge up free radicals and act more effectively than other antioxidants. PEG-C 60-3, a C 60 fullerene derivative, was investigated against β-amyloid (Aβ) 25-35-induced toxicity toward Neuro-2A cells in this study. PEG-C 60-3 reduced Aβ 25-35-induced cytotoxicity, which showed an increasing cell viability with C 60 and Aβ 25-35 co-treated cells. Moreover, the intracellular reactive oxygen species (ROS) accumulation caused by Aβ-treated Neuro-2A cells was reduced by PEG-C 60-3 co-treatment. Microarray for the analysis of gene expressions was investigated. Endoplasmic reticulum (ER) stress responsive genes, ion-channel, cell-cycle and anti-oxidant related cell responses were found to be associated with C 60 protective mechanism against Aβ 25-35 treatment. The results offered new comprehension into the possible pathway of Aβ 25-35 gene expression and C 60 protective mechanism. With the understanding of the roles of Aβ and C 60 in cells, we can hopefully provide insight on therapeutic design using C 60 fullerene nanoparticles against Aβ-associated diseases.

Original languageEnglish
JournalJournal of Food and Drug Analysis
Volume19
Issue number2
Publication statusPublished - Jun 2011

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Keywords

  • Amyloid
  • Apoptosis
  • Fullerene
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

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