C 60 fullerene nanoparticle prevents β-amyloid peptide induced cytotoxicity in neuro 2A cells

Tan Yi Lu, Pai Feng Kao, Chi-Ming Lee, Sheng Tung Huang, Chun Mao Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Oxidative stress, which is an early determinant of Alzheimer's disease (AD), is involved in mediating neuronal apoptosis in Aβ-induced cell death. C 60 fullerenes are known to behave like a "radical sponge" as they can sponge up free radicals and act more effectively than other antioxidants. PEG-C 60-3, a C 60 fullerene derivative, was investigated against β-amyloid (Aβ) 25-35-induced toxicity toward Neuro-2A cells in this study. PEG-C 60-3 reduced Aβ 25-35-induced cytotoxicity, which showed an increasing cell viability with C 60 and Aβ 25-35 co-treated cells. Moreover, the intracellular reactive oxygen species (ROS) accumulation caused by Aβ-treated Neuro-2A cells was reduced by PEG-C 60-3 co-treatment. Microarray for the analysis of gene expressions was investigated. Endoplasmic reticulum (ER) stress responsive genes, ion-channel, cell-cycle and anti-oxidant related cell responses were found to be associated with C 60 protective mechanism against Aβ 25-35 treatment. The results offered new comprehension into the possible pathway of Aβ 25-35 gene expression and C 60 protective mechanism. With the understanding of the roles of Aβ and C 60 in cells, we can hopefully provide insight on therapeutic design using C 60 fullerene nanoparticles against Aβ-associated diseases.

Original languageEnglish
JournalJournal of Food and Drug Analysis
Volume19
Issue number2
Publication statusPublished - Jun 2011

Fingerprint

fullerene
nanoparticles
amyloid
Amyloid
Nanoparticles
cytotoxicity
peptides
Peptides
Porifera
cells
Gene Expression
gene expression
Endoplasmic Reticulum Stress
ion channels
Alzheimer disease
Microarray Analysis
Ion Channels
Oxidants
oxidants
endoplasmic reticulum

Keywords

  • Amyloid
  • Apoptosis
  • Fullerene
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

C 60 fullerene nanoparticle prevents β-amyloid peptide induced cytotoxicity in neuro 2A cells. / Lu, Tan Yi; Kao, Pai Feng; Lee, Chi-Ming; Huang, Sheng Tung; Lin, Chun Mao.

In: Journal of Food and Drug Analysis, Vol. 19, No. 2, 06.2011.

Research output: Contribution to journalArticle

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AU - Lin, Chun Mao

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N2 - Oxidative stress, which is an early determinant of Alzheimer's disease (AD), is involved in mediating neuronal apoptosis in Aβ-induced cell death. C 60 fullerenes are known to behave like a "radical sponge" as they can sponge up free radicals and act more effectively than other antioxidants. PEG-C 60-3, a C 60 fullerene derivative, was investigated against β-amyloid (Aβ) 25-35-induced toxicity toward Neuro-2A cells in this study. PEG-C 60-3 reduced Aβ 25-35-induced cytotoxicity, which showed an increasing cell viability with C 60 and Aβ 25-35 co-treated cells. Moreover, the intracellular reactive oxygen species (ROS) accumulation caused by Aβ-treated Neuro-2A cells was reduced by PEG-C 60-3 co-treatment. Microarray for the analysis of gene expressions was investigated. Endoplasmic reticulum (ER) stress responsive genes, ion-channel, cell-cycle and anti-oxidant related cell responses were found to be associated with C 60 protective mechanism against Aβ 25-35 treatment. The results offered new comprehension into the possible pathway of Aβ 25-35 gene expression and C 60 protective mechanism. With the understanding of the roles of Aβ and C 60 in cells, we can hopefully provide insight on therapeutic design using C 60 fullerene nanoparticles against Aβ-associated diseases.

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