By inhibiting snail signaling and miR-23a-3p, osthole suppresses the EMT-mediated metastatic ability in prostate cancer

Yu-Ching Wen, Wei Jiunn Lee, Peng Tan, Shun Fa Yang, Michael Hsiao, Liang Ming Lee, Ming Hsien Chien

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Here we showed that Osthole, 7-methoxy-8-(3-methyl-2-butenyl) coumarin, a bioactive coumarin derivative extracted from medicinal plants, inhibited migration, invasion, epithelial to mesenchymal transition (EMT) in androgen-independent prostate cancer (AIPC) cells in vitro and metastasis of AIPC in vivo. In patients, high Snail levels were correlated with a higher histological Gleason sum and poor survival rates. Osthole inhibited the TGF-β/Akt/MAPK pathways, reduced Snail-DNAbinding activity and induced E-cadherin. We found that osthole decreased miR-23a-3p. Ectopic miR-23a-3p suppressed E-cadherin 3' untranslated region reporter activity and E-cadherin expression, and relieved the motility suppression caused by osthole treatment.

Original languageEnglish
Pages (from-to)21120-21136
Number of pages17
JournalOncotarget
Volume6
Issue number25
DOIs
Publication statusPublished - 2015

Keywords

  • EMT
  • MiR-23a-3p
  • Osthole
  • Prostate cancer
  • Snail

ASJC Scopus subject areas

  • Oncology

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