Butylidenephthalide blocks potassium channels and enhances basal tension in isolated guinea-pig trachea

Hsin Te Hsu, You Lan Yang, Wan Chen Chen, Chi Ming Chen, Wun-Chang Ko

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Butylidenephthalide (Bdph, 30300 M), a constituent of Ligusticum chuanxiong Hort., significantly enhanced tension in isolated guinea-pig trachea. In this study, we investigate the mechanism(s) of Bdph-induced contraction in the tissue. Isolated trachea was bathed in 5 mL of Krebs solution containing indomethacin (3 M), and its tension changes were isometrically recorded. Cromakalim (3 M), an ATP-dependent K+ channel opener, significantly antagonized the Bdph-induced enhancement of baseline tension. Bdph (300 M) also significantly antagonized cromakalim-induced relaxation. Bdph (300 M) did not significantly influence the antagonistic effects of glibenclamide (GBC, 1 M) and tetraethylammonium (TEA, 8 mM) against the cromakalim-induced relaxation. However, Bdph (300 M) and 4-aminopiridine (4-AP, 5 mM), a blocker of K v1 family of K+ channels, in combination significantly rightward shifted the log concentration-relaxation curve of cromakalim. The antagonistic effect of the combination almost equals the sum of the individual effects of Bdph and 4-AP, suggesting that the antagonistic mechanism of Bdph may be similar to that of 4-AP. All calcium channel blockers influenced neither the baseline tension nor antagonistic effect of Bdph against cromakalim. In conclusion, Bdph may be similar to 4-AP, a blocker of Kv1 family of K+ channels, to enhance the baseline tension of guinea-pig trachea.

Original languageEnglish
Article number875230
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Cromakalim
Potassium Channels
Trachea
Guinea Pigs
Ligusticum
Tetraethylammonium
Glyburide
Calcium Channel Blockers
Indomethacin
Adenosine Triphosphate
butylidenephthalide
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Butylidenephthalide blocks potassium channels and enhances basal tension in isolated guinea-pig trachea. / Hsu, Hsin Te; Yang, You Lan; Chen, Wan Chen; Chen, Chi Ming; Ko, Wun-Chang.

In: BioMed Research International, Vol. 2014, 875230, 2014.

Research output: Contribution to journalArticle

@article{bac7f71eebfc420bab1eb108d621cde6,
title = "Butylidenephthalide blocks potassium channels and enhances basal tension in isolated guinea-pig trachea",
abstract = "Butylidenephthalide (Bdph, 30300 M), a constituent of Ligusticum chuanxiong Hort., significantly enhanced tension in isolated guinea-pig trachea. In this study, we investigate the mechanism(s) of Bdph-induced contraction in the tissue. Isolated trachea was bathed in 5 mL of Krebs solution containing indomethacin (3 M), and its tension changes were isometrically recorded. Cromakalim (3 M), an ATP-dependent K+ channel opener, significantly antagonized the Bdph-induced enhancement of baseline tension. Bdph (300 M) also significantly antagonized cromakalim-induced relaxation. Bdph (300 M) did not significantly influence the antagonistic effects of glibenclamide (GBC, 1 M) and tetraethylammonium (TEA, 8 mM) against the cromakalim-induced relaxation. However, Bdph (300 M) and 4-aminopiridine (4-AP, 5 mM), a blocker of K v1 family of K+ channels, in combination significantly rightward shifted the log concentration-relaxation curve of cromakalim. The antagonistic effect of the combination almost equals the sum of the individual effects of Bdph and 4-AP, suggesting that the antagonistic mechanism of Bdph may be similar to that of 4-AP. All calcium channel blockers influenced neither the baseline tension nor antagonistic effect of Bdph against cromakalim. In conclusion, Bdph may be similar to 4-AP, a blocker of Kv1 family of K+ channels, to enhance the baseline tension of guinea-pig trachea.",
author = "Hsu, {Hsin Te} and Yang, {You Lan} and Chen, {Wan Chen} and Chen, {Chi Ming} and Wun-Chang Ko",
year = "2014",
doi = "10.1155/2014/875230",
language = "English",
volume = "2014",
journal = "BioMed Research International",
issn = "2314-6133",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Butylidenephthalide blocks potassium channels and enhances basal tension in isolated guinea-pig trachea

AU - Hsu, Hsin Te

AU - Yang, You Lan

AU - Chen, Wan Chen

AU - Chen, Chi Ming

AU - Ko, Wun-Chang

PY - 2014

Y1 - 2014

N2 - Butylidenephthalide (Bdph, 30300 M), a constituent of Ligusticum chuanxiong Hort., significantly enhanced tension in isolated guinea-pig trachea. In this study, we investigate the mechanism(s) of Bdph-induced contraction in the tissue. Isolated trachea was bathed in 5 mL of Krebs solution containing indomethacin (3 M), and its tension changes were isometrically recorded. Cromakalim (3 M), an ATP-dependent K+ channel opener, significantly antagonized the Bdph-induced enhancement of baseline tension. Bdph (300 M) also significantly antagonized cromakalim-induced relaxation. Bdph (300 M) did not significantly influence the antagonistic effects of glibenclamide (GBC, 1 M) and tetraethylammonium (TEA, 8 mM) against the cromakalim-induced relaxation. However, Bdph (300 M) and 4-aminopiridine (4-AP, 5 mM), a blocker of K v1 family of K+ channels, in combination significantly rightward shifted the log concentration-relaxation curve of cromakalim. The antagonistic effect of the combination almost equals the sum of the individual effects of Bdph and 4-AP, suggesting that the antagonistic mechanism of Bdph may be similar to that of 4-AP. All calcium channel blockers influenced neither the baseline tension nor antagonistic effect of Bdph against cromakalim. In conclusion, Bdph may be similar to 4-AP, a blocker of Kv1 family of K+ channels, to enhance the baseline tension of guinea-pig trachea.

AB - Butylidenephthalide (Bdph, 30300 M), a constituent of Ligusticum chuanxiong Hort., significantly enhanced tension in isolated guinea-pig trachea. In this study, we investigate the mechanism(s) of Bdph-induced contraction in the tissue. Isolated trachea was bathed in 5 mL of Krebs solution containing indomethacin (3 M), and its tension changes were isometrically recorded. Cromakalim (3 M), an ATP-dependent K+ channel opener, significantly antagonized the Bdph-induced enhancement of baseline tension. Bdph (300 M) also significantly antagonized cromakalim-induced relaxation. Bdph (300 M) did not significantly influence the antagonistic effects of glibenclamide (GBC, 1 M) and tetraethylammonium (TEA, 8 mM) against the cromakalim-induced relaxation. However, Bdph (300 M) and 4-aminopiridine (4-AP, 5 mM), a blocker of K v1 family of K+ channels, in combination significantly rightward shifted the log concentration-relaxation curve of cromakalim. The antagonistic effect of the combination almost equals the sum of the individual effects of Bdph and 4-AP, suggesting that the antagonistic mechanism of Bdph may be similar to that of 4-AP. All calcium channel blockers influenced neither the baseline tension nor antagonistic effect of Bdph against cromakalim. In conclusion, Bdph may be similar to 4-AP, a blocker of Kv1 family of K+ channels, to enhance the baseline tension of guinea-pig trachea.

UR - http://www.scopus.com/inward/record.url?scp=84904797765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904797765&partnerID=8YFLogxK

U2 - 10.1155/2014/875230

DO - 10.1155/2014/875230

M3 - Article

C2 - 25114927

AN - SCOPUS:84904797765

VL - 2014

JO - BioMed Research International

JF - BioMed Research International

SN - 2314-6133

M1 - 875230

ER -