Brown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cells

Szu Yuan Wu, Ming De Yan, Alexander T H Wu, Kevin Sheng Po Yuan, Shing Hwa Liu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, we observed that brown seaweed fucoidan inhibited human breast cancer progression by upregulating microRNA (miR)-29c and downregulating miR-17-5p, thereby suppressing their target genes, a disintegrin and metalloproteinase 12 (ADAM12) and phosphatase and tensin homolog (PTEN), respectively. Moreover, fucoidan reduced the luciferase activity of 3'-untranslated region reporter; treatment of cells with the miR-29c mimic or miR-17-5p inhibitor also produced similar results. These effects of fucoidan inhibited the epithelial-mesenchymal transition in breast cancer cells, as evidenced by an increase in E-cadherin and a drop in N-cadherin, and inhibited breast cancer cell survival, as evidenced by the activation of the phosphoinositide 3-kinase/Akt pathway. Taken together, these findings demonstrate that fucoidan inhibits breast cancer progression by regulating the miR-29c/ADAM12 and miR-17-5p/PTEN axes. Fucoidan is a potential chemopreventive/chemotherapeutic agent for breast cancer.

Original languageEnglish
Pages (from-to)2408-2419
Number of pages12
JournalJournal of Cancer
Volume7
Issue number15
DOIs
Publication statusPublished - 2016

Fingerprint

Disintegrins
Seaweed
Metalloproteases
MicroRNAs
Phosphoric Monoester Hydrolases
Breast Neoplasms
Neoplasms
Cadherins
Epithelial-Mesenchymal Transition
1-Phosphatidylinositol 4-Kinase
3' Untranslated Regions
Luciferases
fucoidan
Tensins
Cell Survival
Down-Regulation
Genes

Keywords

  • ADAM12
  • Breast cancer cells
  • Fucoidan
  • MiR-17-5p
  • MiR-29c
  • PTEN

ASJC Scopus subject areas

  • Oncology

Cite this

Brown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cells. / Wu, Szu Yuan; Yan, Ming De; Wu, Alexander T H; Yuan, Kevin Sheng Po; Liu, Shing Hwa.

In: Journal of Cancer, Vol. 7, No. 15, 2016, p. 2408-2419.

Research output: Contribution to journalArticle

@article{44846d0bf96841af8af0be840e91a3c8,
title = "Brown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cells",
abstract = "In this study, we observed that brown seaweed fucoidan inhibited human breast cancer progression by upregulating microRNA (miR)-29c and downregulating miR-17-5p, thereby suppressing their target genes, a disintegrin and metalloproteinase 12 (ADAM12) and phosphatase and tensin homolog (PTEN), respectively. Moreover, fucoidan reduced the luciferase activity of 3'-untranslated region reporter; treatment of cells with the miR-29c mimic or miR-17-5p inhibitor also produced similar results. These effects of fucoidan inhibited the epithelial-mesenchymal transition in breast cancer cells, as evidenced by an increase in E-cadherin and a drop in N-cadherin, and inhibited breast cancer cell survival, as evidenced by the activation of the phosphoinositide 3-kinase/Akt pathway. Taken together, these findings demonstrate that fucoidan inhibits breast cancer progression by regulating the miR-29c/ADAM12 and miR-17-5p/PTEN axes. Fucoidan is a potential chemopreventive/chemotherapeutic agent for breast cancer.",
keywords = "ADAM12, Breast cancer cells, Fucoidan, MiR-17-5p, MiR-29c, PTEN",
author = "Wu, {Szu Yuan} and Yan, {Ming De} and Wu, {Alexander T H} and Yuan, {Kevin Sheng Po} and Liu, {Shing Hwa}",
year = "2016",
doi = "10.7150/jca.15703",
language = "English",
volume = "7",
pages = "2408--2419",
journal = "Journal of Cancer",
issn = "1837-9664",
publisher = "Ivyspring International Publisher",
number = "15",

}

TY - JOUR

T1 - Brown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cells

AU - Wu, Szu Yuan

AU - Yan, Ming De

AU - Wu, Alexander T H

AU - Yuan, Kevin Sheng Po

AU - Liu, Shing Hwa

PY - 2016

Y1 - 2016

N2 - In this study, we observed that brown seaweed fucoidan inhibited human breast cancer progression by upregulating microRNA (miR)-29c and downregulating miR-17-5p, thereby suppressing their target genes, a disintegrin and metalloproteinase 12 (ADAM12) and phosphatase and tensin homolog (PTEN), respectively. Moreover, fucoidan reduced the luciferase activity of 3'-untranslated region reporter; treatment of cells with the miR-29c mimic or miR-17-5p inhibitor also produced similar results. These effects of fucoidan inhibited the epithelial-mesenchymal transition in breast cancer cells, as evidenced by an increase in E-cadherin and a drop in N-cadherin, and inhibited breast cancer cell survival, as evidenced by the activation of the phosphoinositide 3-kinase/Akt pathway. Taken together, these findings demonstrate that fucoidan inhibits breast cancer progression by regulating the miR-29c/ADAM12 and miR-17-5p/PTEN axes. Fucoidan is a potential chemopreventive/chemotherapeutic agent for breast cancer.

AB - In this study, we observed that brown seaweed fucoidan inhibited human breast cancer progression by upregulating microRNA (miR)-29c and downregulating miR-17-5p, thereby suppressing their target genes, a disintegrin and metalloproteinase 12 (ADAM12) and phosphatase and tensin homolog (PTEN), respectively. Moreover, fucoidan reduced the luciferase activity of 3'-untranslated region reporter; treatment of cells with the miR-29c mimic or miR-17-5p inhibitor also produced similar results. These effects of fucoidan inhibited the epithelial-mesenchymal transition in breast cancer cells, as evidenced by an increase in E-cadherin and a drop in N-cadherin, and inhibited breast cancer cell survival, as evidenced by the activation of the phosphoinositide 3-kinase/Akt pathway. Taken together, these findings demonstrate that fucoidan inhibits breast cancer progression by regulating the miR-29c/ADAM12 and miR-17-5p/PTEN axes. Fucoidan is a potential chemopreventive/chemotherapeutic agent for breast cancer.

KW - ADAM12

KW - Breast cancer cells

KW - Fucoidan

KW - MiR-17-5p

KW - MiR-29c

KW - PTEN

UR - http://www.scopus.com/inward/record.url?scp=85006963777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006963777&partnerID=8YFLogxK

U2 - 10.7150/jca.15703

DO - 10.7150/jca.15703

M3 - Article

AN - SCOPUS:85006963777

VL - 7

SP - 2408

EP - 2419

JO - Journal of Cancer

JF - Journal of Cancer

SN - 1837-9664

IS - 15

ER -