Abstract
The derivation of mesenchymal progenitors from human embryonic stem cells (hESCs) has recently been reported. We studied the immune characteristics of these hESC-de rived mesenchymal progenitors (EMPs) and their interactions with T lymphocytes and natural killer cells (NKs), two populations of lymphocytes with important roles in transplantation immunology. EMPs express a number of bone marrow mesenchymal stromal cell (BMMSC) markers, as well as the hESC marker SSEA-4. Immunologically, EMPs do not express HLA-DR or costimulatory molecules. On the other hand, HLA-G, a nonclassic MHC I protein involved in mediating maternal-fetal tolerance, can be found on the surface of EMPs, and its expression is increased after interferon-γ stimulation. EMPs can suppress CD4+ or CD8+ lymphocyte proliferation, similar to BMMSCs. However, EMPs are more resistant to NK-mediated lysis than BMMSCs and can suppress the cytotoxic effects of activated NKs, as well as downregu-lating the NK-activating receptors NKp30 and NKp46. With their broad immunosuppressive properties, EMPs may represent a new potential cell source for therapeutic use.
| Original language | English |
|---|---|
| Pages (from-to) | 451-456 |
| Number of pages | 6 |
| Journal | Stem Cells |
| Volume | 27 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2009 |
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Keywords
- HLA-G natural killer cells
- Human embryonic stem cells
- Immunosuppression
- Mesenchymal stromal cells
- T lymphocytes
ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
- Molecular Medicine
Cite this
Brief report-Human embryonic stem cell-derived mesenchymal progenitors possess strong immunosuppressive effects toward natural killer cells as well as T lymphocytes. / Yen, B. Linju; Chan, Jung Chang; Liu, Ko Jiunn; Yao, Chang Chen; Hu, Hsin I.; Chi-Huey, Bai; Yen, Men Luh.
In: Stem Cells, Vol. 27, No. 2, 02.2009, p. 451-456.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Brief report-Human embryonic stem cell-derived mesenchymal progenitors possess strong immunosuppressive effects toward natural killer cells as well as T lymphocytes
AU - Yen, B. Linju
AU - Chan, Jung Chang
AU - Liu, Ko Jiunn
AU - Yao, Chang Chen
AU - Hu, Hsin I.
AU - Chi-Huey, Bai
AU - Yen, Men Luh
PY - 2009/2
Y1 - 2009/2
N2 - The derivation of mesenchymal progenitors from human embryonic stem cells (hESCs) has recently been reported. We studied the immune characteristics of these hESC-de rived mesenchymal progenitors (EMPs) and their interactions with T lymphocytes and natural killer cells (NKs), two populations of lymphocytes with important roles in transplantation immunology. EMPs express a number of bone marrow mesenchymal stromal cell (BMMSC) markers, as well as the hESC marker SSEA-4. Immunologically, EMPs do not express HLA-DR or costimulatory molecules. On the other hand, HLA-G, a nonclassic MHC I protein involved in mediating maternal-fetal tolerance, can be found on the surface of EMPs, and its expression is increased after interferon-γ stimulation. EMPs can suppress CD4+ or CD8+ lymphocyte proliferation, similar to BMMSCs. However, EMPs are more resistant to NK-mediated lysis than BMMSCs and can suppress the cytotoxic effects of activated NKs, as well as downregu-lating the NK-activating receptors NKp30 and NKp46. With their broad immunosuppressive properties, EMPs may represent a new potential cell source for therapeutic use.
AB - The derivation of mesenchymal progenitors from human embryonic stem cells (hESCs) has recently been reported. We studied the immune characteristics of these hESC-de rived mesenchymal progenitors (EMPs) and their interactions with T lymphocytes and natural killer cells (NKs), two populations of lymphocytes with important roles in transplantation immunology. EMPs express a number of bone marrow mesenchymal stromal cell (BMMSC) markers, as well as the hESC marker SSEA-4. Immunologically, EMPs do not express HLA-DR or costimulatory molecules. On the other hand, HLA-G, a nonclassic MHC I protein involved in mediating maternal-fetal tolerance, can be found on the surface of EMPs, and its expression is increased after interferon-γ stimulation. EMPs can suppress CD4+ or CD8+ lymphocyte proliferation, similar to BMMSCs. However, EMPs are more resistant to NK-mediated lysis than BMMSCs and can suppress the cytotoxic effects of activated NKs, as well as downregu-lating the NK-activating receptors NKp30 and NKp46. With their broad immunosuppressive properties, EMPs may represent a new potential cell source for therapeutic use.
KW - HLA-G natural killer cells
KW - Human embryonic stem cells
KW - Immunosuppression
KW - Mesenchymal stromal cells
KW - T lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=62549157393&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62549157393&partnerID=8YFLogxK
U2 - 10.1634/stemcells.2008-0390
DO - 10.1634/stemcells.2008-0390
M3 - Article
C2 - 18988708
AN - SCOPUS:62549157393
VL - 27
SP - 451
EP - 456
JO - Stem Cells
JF - Stem Cells
SN - 1066-5099
IS - 2
ER -