Brazilin ameliorates high glucose-induced vascular inflammation via inhibiting ROS and CAMs production in human umbilical vein endothelial cells

Thanasekaran Jayakumar, Chao Chien Chang, Shoei Loong Lin, Yung Kai Huang, Chien Ming Hu, Antoinet Ramola Elizebeth, Shih Chang Lin, Cheuk-Sing Choy

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Vascular inflammatory process has been suggested to play a key role in the initiation and progression of atherosclerosis, a major complication of diabetes mellitus. Recent studies have shown that brazilin exhibits antihepatotoxic, antiplatelet, cancer preventive, or anti-inflammatory properties. Thus, we investigated whether brazilin suppresses vascular inflammatory process induced by high glucose (HG) in cultured human umbilical vein endothelial cells (HUVEC). HG induced nitrite production, lipid peroxidation, and intracellular reactive oxygen species formation in HUVEC cells, which was reversed by brazilin. Western blot analysis revealed that brazilin markedly inhibited HG-induced phosphorylation of endothelial nitric oxide synthase. Besides, we investigated the effects of brazilin on the MAPK signal transduction pathway because MAPK families are associated with vascular inflammation under stress. Brazilin blocked HG-induced phosphorylation of extracellular signal-regulated kinase and transcription factor NF-B. Furthermore, brazilin concentration-dependently attenuated cell adhesion molecules (ICAM-1 and VCAM-1) expression induced by various concentrations of HG in HUVEC. Taken together, the present data suggested that brazilin could suppress high glucose-induced vascular inflammatory process, which may be closely related with the inhibition of oxidative stress, CAMs expression, and NF-B activation in HUVEC. Our findings may highlight a new therapeutic intervention for the prevention of vascular diseases.

Original languageEnglish
Article number403703
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

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Endothelial cells
Human Umbilical Vein Endothelial Cells
Computer aided manufacturing
Blood Vessels
Inflammation
Glucose
Phosphorylation
Signal transduction
Oxidative stress
Vascular Cell Adhesion Molecule-1
brazilin
Nitric Oxide Synthase Type III
Extracellular Signal-Regulated MAP Kinases
Cell Adhesion Molecules
Intercellular Adhesion Molecule-1
Diabetes Complications
Medical problems
Nitrites
Vascular Diseases
Lipid Peroxidation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Brazilin ameliorates high glucose-induced vascular inflammation via inhibiting ROS and CAMs production in human umbilical vein endothelial cells. / Jayakumar, Thanasekaran; Chang, Chao Chien; Lin, Shoei Loong; Huang, Yung Kai; Hu, Chien Ming; Elizebeth, Antoinet Ramola; Lin, Shih Chang; Choy, Cheuk-Sing.

In: BioMed Research International, Vol. 2014, 403703, 2014.

Research output: Contribution to journalArticle

Jayakumar, Thanasekaran ; Chang, Chao Chien ; Lin, Shoei Loong ; Huang, Yung Kai ; Hu, Chien Ming ; Elizebeth, Antoinet Ramola ; Lin, Shih Chang ; Choy, Cheuk-Sing. / Brazilin ameliorates high glucose-induced vascular inflammation via inhibiting ROS and CAMs production in human umbilical vein endothelial cells. In: BioMed Research International. 2014 ; Vol. 2014.
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