Brain-specific 1B promoter of FGF1 gene facilitates the isolation of neural stem/progenitor cells with self-renewal and multipotent capacities

Yi Chao Hsu, Don Ching Lee, Su Liang Chen, Wei Chih Liao, Jia Wei Lin, Wen Ta Chiu, Ing Ming Chiu

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Fibroblast growth factor 1 (FGF1) has been shown to maintain proliferation and self-renewal capacities of neural stem/progenitor cells (NSPCs) in vitro. We have previously identified FGF1B as the major transcript of FGF1 gene expressed exclusively in brain areas that are known to be abundant for NSPCs in vivo. The 540-bp (-540 to +31) sequence upstream of the 1B transcription start site (F1B) is sufficient to drive the expression of a heterologous luciferase reporter in cultured cells. In this study, we report a direct genetic and functional approach to isolate F1B(+) NSPCs using green fluorescent protein (GFP) reporter gene under the control of human F1B promoter. The F1B-GFP reporter could facilitate the isolation of NSPCs with self-renewal and multipotent capacities from human glioblastoma tissues, developing or adult mouse brains by fluorescence-activated cell sorting. Future work elucidating the mechanisms that control FGF1B expression will help to identify new NSPC-related genes.

Original languageEnglish
Pages (from-to)302-314
Number of pages13
JournalDevelopmental Dynamics
Volume238
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Fibroblast Growth Factor 1
Neural Stem Cells
Stem Cells
Brain
Genes
Green Fluorescent Proteins
Transcription Initiation Site
Glioblastoma
Luciferases
Reporter Genes
Cultured Cells
Flow Cytometry
Cell Self Renewal

Keywords

  • CD133
  • F1B
  • FGF1
  • Neural progenitors
  • Neural stem cells

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Brain-specific 1B promoter of FGF1 gene facilitates the isolation of neural stem/progenitor cells with self-renewal and multipotent capacities. / Hsu, Yi Chao; Lee, Don Ching; Chen, Su Liang; Liao, Wei Chih; Lin, Jia Wei; Chiu, Wen Ta; Chiu, Ing Ming.

In: Developmental Dynamics, Vol. 238, No. 2, 02.2009, p. 302-314.

Research output: Contribution to journalArticle

Hsu, Yi Chao ; Lee, Don Ching ; Chen, Su Liang ; Liao, Wei Chih ; Lin, Jia Wei ; Chiu, Wen Ta ; Chiu, Ing Ming. / Brain-specific 1B promoter of FGF1 gene facilitates the isolation of neural stem/progenitor cells with self-renewal and multipotent capacities. In: Developmental Dynamics. 2009 ; Vol. 238, No. 2. pp. 302-314.
@article{0301760a9350421cb230f35934983342,
title = "Brain-specific 1B promoter of FGF1 gene facilitates the isolation of neural stem/progenitor cells with self-renewal and multipotent capacities",
abstract = "Fibroblast growth factor 1 (FGF1) has been shown to maintain proliferation and self-renewal capacities of neural stem/progenitor cells (NSPCs) in vitro. We have previously identified FGF1B as the major transcript of FGF1 gene expressed exclusively in brain areas that are known to be abundant for NSPCs in vivo. The 540-bp (-540 to +31) sequence upstream of the 1B transcription start site (F1B) is sufficient to drive the expression of a heterologous luciferase reporter in cultured cells. In this study, we report a direct genetic and functional approach to isolate F1B(+) NSPCs using green fluorescent protein (GFP) reporter gene under the control of human F1B promoter. The F1B-GFP reporter could facilitate the isolation of NSPCs with self-renewal and multipotent capacities from human glioblastoma tissues, developing or adult mouse brains by fluorescence-activated cell sorting. Future work elucidating the mechanisms that control FGF1B expression will help to identify new NSPC-related genes.",
keywords = "CD133, F1B, FGF1, Neural progenitors, Neural stem cells",
author = "Hsu, {Yi Chao} and Lee, {Don Ching} and Chen, {Su Liang} and Liao, {Wei Chih} and Lin, {Jia Wei} and Chiu, {Wen Ta} and Chiu, {Ing Ming}",
year = "2009",
month = "2",
doi = "10.1002/dvdy.21753",
language = "English",
volume = "238",
pages = "302--314",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Brain-specific 1B promoter of FGF1 gene facilitates the isolation of neural stem/progenitor cells with self-renewal and multipotent capacities

AU - Hsu, Yi Chao

AU - Lee, Don Ching

AU - Chen, Su Liang

AU - Liao, Wei Chih

AU - Lin, Jia Wei

AU - Chiu, Wen Ta

AU - Chiu, Ing Ming

PY - 2009/2

Y1 - 2009/2

N2 - Fibroblast growth factor 1 (FGF1) has been shown to maintain proliferation and self-renewal capacities of neural stem/progenitor cells (NSPCs) in vitro. We have previously identified FGF1B as the major transcript of FGF1 gene expressed exclusively in brain areas that are known to be abundant for NSPCs in vivo. The 540-bp (-540 to +31) sequence upstream of the 1B transcription start site (F1B) is sufficient to drive the expression of a heterologous luciferase reporter in cultured cells. In this study, we report a direct genetic and functional approach to isolate F1B(+) NSPCs using green fluorescent protein (GFP) reporter gene under the control of human F1B promoter. The F1B-GFP reporter could facilitate the isolation of NSPCs with self-renewal and multipotent capacities from human glioblastoma tissues, developing or adult mouse brains by fluorescence-activated cell sorting. Future work elucidating the mechanisms that control FGF1B expression will help to identify new NSPC-related genes.

AB - Fibroblast growth factor 1 (FGF1) has been shown to maintain proliferation and self-renewal capacities of neural stem/progenitor cells (NSPCs) in vitro. We have previously identified FGF1B as the major transcript of FGF1 gene expressed exclusively in brain areas that are known to be abundant for NSPCs in vivo. The 540-bp (-540 to +31) sequence upstream of the 1B transcription start site (F1B) is sufficient to drive the expression of a heterologous luciferase reporter in cultured cells. In this study, we report a direct genetic and functional approach to isolate F1B(+) NSPCs using green fluorescent protein (GFP) reporter gene under the control of human F1B promoter. The F1B-GFP reporter could facilitate the isolation of NSPCs with self-renewal and multipotent capacities from human glioblastoma tissues, developing or adult mouse brains by fluorescence-activated cell sorting. Future work elucidating the mechanisms that control FGF1B expression will help to identify new NSPC-related genes.

KW - CD133

KW - F1B

KW - FGF1

KW - Neural progenitors

KW - Neural stem cells

UR - http://www.scopus.com/inward/record.url?scp=59649084032&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59649084032&partnerID=8YFLogxK

U2 - 10.1002/dvdy.21753

DO - 10.1002/dvdy.21753

M3 - Article

C2 - 18855895

AN - SCOPUS:59649084032

VL - 238

SP - 302

EP - 314

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 2

ER -