BPDE-like DNA adduct level in oral tissue may act as a risk biomarker of oral cancer

Chun Yi Chuang, Jai Nien Tung, Mao Chang Su, Buor Chang Wu, Chun Han Hsin, Yi Juai Chen, Kun Tu Yeh, Huei Lee, Ya Wen Cheng

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Objective: Most reports have shown that PAH-related DNA adducts are positively correlated with the smoking status of oral cancer patients. However, these reports did not focus on a specific carcinogen in cigarette smoke. The purpose of this study was to elucidate the role of the BPDE (7,8-dihydroxy-anti- 9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene)-DNA adduct in the development of oral cancer in Taiwanese patients. Design: We enrolled 158 oral cancer patients and 64 non-cancer controls to investigate whether there were differences in susceptibility to cigarette smoke exposure in the formation of DNA adducts between cancer patients and controls. Immunohistochemistry and ELISA (enzyme-linked immunosorbent assay) were used to evaluate BPDE-DNA adduct levels in this study. Results: Our data showed that the BPDE-DNA adduct levels were positively correlated with gender, smoking status, betel nut chewing and alcohol consumption. The difference in DNA adduct levels could be explained by genetic polymorphisms of glutathione S-transferase M1 (GSTM1), but not by cytochrome P-4501A1 (CYP1A1). Patients with high DNA adduct levels (≧34.03 adducts/108 nucleotides) had an approximately 9.936-fold risk of oral cancer compared with those with low DNA adduct levels (8 nucleotides) (p <0.001). Conclusions: We suggest that genetic background and carcinogen exposure may increase the risk of developing oral cancer.

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalArchives of Oral Biology
Volume58
Issue number1
DOIs
Publication statusPublished - Jan 2013

Keywords

  • DNA adducts
  • Oral cancer
  • Risk factor

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

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