Both allergen-specific CD4 and CD8 type 2 T cells decreased in asthmatic children with immunotherapy

Chi Ling Fu, Yi Ling Ye, Yueh L. Lee, Bor Luen Chiang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Allergen-specific immunotherapy (IT) has been used for the treatment of atopic diseases since the turn of this century. The precise working mechanisms, however, remain to be clarified. The aim of this study was to investigate the role of particular subsets of allergen -specific T cells in the non-atopic individuals, untreated asthmatic children and the asthmatic children receiving immunotherapy. We collected peripheral blood from 16 untreated asthmatic children and 17 asthmatic children receiving immunotherapy over one and half years. All the patients were sensitive to mite allergen. Peripheral blood mononuclear cells (PBMC) were isolated and, in vitro, stimulated with crude mite extract to enrich the mite-specific T-cell population. After 14 days, the enriched mite-specific T cells were stimulated with phorbol-12-myristate-13-acetate (PMA) and ionomycin for intracellular detection of cytokines such as IFN-γ, IL-4 in CD4+ and CD8+ T lymphocytes. The data here demonstrated that the levels of mite-specific IgG4 and IgA increased significantly in asthmatic children after immunotherapy. In addition, both IL-4 expressing CD4+ and CD8+ T cells were significantly lower in asthmatic children after immunotherapy compared with those of before treatment and the normal control (p <0.05). In contrast, the frequency of IFN-γ expressing CD4+ and CD8+ T cells did not significantly differ between untreated and SIT-treated groups. All these data suggested that decreased Type 2 CD4+ and CD8 + T cells might be closely correlated with the regulatory mechanisms of immunotherapy.

Original languageEnglish
Pages (from-to)284-291
Number of pages8
JournalPediatric Allergy and Immunology
Volume14
Issue number4
DOIs
Publication statusPublished - Aug 2003
Externally publishedYes

Fingerprint

Immunotherapy
Allergens
Mites
T-Lymphocytes
Interleukin-4
Immunologic Desensitization
Ionomycin
Complex Mixtures
Immunoglobulin A
Blood Cells
Acetates
Immunoglobulin G
Cytokines
Therapeutics
Population

Keywords

  • CD4 T cells
  • CD8 T cells
  • Immunotherapy
  • Intracellular staining

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pediatrics, Perinatology, and Child Health

Cite this

Both allergen-specific CD4 and CD8 type 2 T cells decreased in asthmatic children with immunotherapy. / Fu, Chi Ling; Ye, Yi Ling; Lee, Yueh L.; Chiang, Bor Luen.

In: Pediatric Allergy and Immunology, Vol. 14, No. 4, 08.2003, p. 284-291.

Research output: Contribution to journalArticle

@article{cdb5aa2678c046faad57410471fd42a1,
title = "Both allergen-specific CD4 and CD8 type 2 T cells decreased in asthmatic children with immunotherapy",
abstract = "Allergen-specific immunotherapy (IT) has been used for the treatment of atopic diseases since the turn of this century. The precise working mechanisms, however, remain to be clarified. The aim of this study was to investigate the role of particular subsets of allergen -specific T cells in the non-atopic individuals, untreated asthmatic children and the asthmatic children receiving immunotherapy. We collected peripheral blood from 16 untreated asthmatic children and 17 asthmatic children receiving immunotherapy over one and half years. All the patients were sensitive to mite allergen. Peripheral blood mononuclear cells (PBMC) were isolated and, in vitro, stimulated with crude mite extract to enrich the mite-specific T-cell population. After 14 days, the enriched mite-specific T cells were stimulated with phorbol-12-myristate-13-acetate (PMA) and ionomycin for intracellular detection of cytokines such as IFN-γ, IL-4 in CD4+ and CD8+ T lymphocytes. The data here demonstrated that the levels of mite-specific IgG4 and IgA increased significantly in asthmatic children after immunotherapy. In addition, both IL-4 expressing CD4+ and CD8+ T cells were significantly lower in asthmatic children after immunotherapy compared with those of before treatment and the normal control (p <0.05). In contrast, the frequency of IFN-γ expressing CD4+ and CD8+ T cells did not significantly differ between untreated and SIT-treated groups. All these data suggested that decreased Type 2 CD4+ and CD8 + T cells might be closely correlated with the regulatory mechanisms of immunotherapy.",
keywords = "CD4 T cells, CD8 T cells, Immunotherapy, Intracellular staining",
author = "Fu, {Chi Ling} and Ye, {Yi Ling} and Lee, {Yueh L.} and Chiang, {Bor Luen}",
year = "2003",
month = "8",
doi = "10.1034/j.1399-3038.2003.00054.x",
language = "English",
volume = "14",
pages = "284--291",
journal = "Pediatric Allergy and Immunology",
issn = "0905-6157",
publisher = "Blackwell Munksgaard",
number = "4",

}

TY - JOUR

T1 - Both allergen-specific CD4 and CD8 type 2 T cells decreased in asthmatic children with immunotherapy

AU - Fu, Chi Ling

AU - Ye, Yi Ling

AU - Lee, Yueh L.

AU - Chiang, Bor Luen

PY - 2003/8

Y1 - 2003/8

N2 - Allergen-specific immunotherapy (IT) has been used for the treatment of atopic diseases since the turn of this century. The precise working mechanisms, however, remain to be clarified. The aim of this study was to investigate the role of particular subsets of allergen -specific T cells in the non-atopic individuals, untreated asthmatic children and the asthmatic children receiving immunotherapy. We collected peripheral blood from 16 untreated asthmatic children and 17 asthmatic children receiving immunotherapy over one and half years. All the patients were sensitive to mite allergen. Peripheral blood mononuclear cells (PBMC) were isolated and, in vitro, stimulated with crude mite extract to enrich the mite-specific T-cell population. After 14 days, the enriched mite-specific T cells were stimulated with phorbol-12-myristate-13-acetate (PMA) and ionomycin for intracellular detection of cytokines such as IFN-γ, IL-4 in CD4+ and CD8+ T lymphocytes. The data here demonstrated that the levels of mite-specific IgG4 and IgA increased significantly in asthmatic children after immunotherapy. In addition, both IL-4 expressing CD4+ and CD8+ T cells were significantly lower in asthmatic children after immunotherapy compared with those of before treatment and the normal control (p <0.05). In contrast, the frequency of IFN-γ expressing CD4+ and CD8+ T cells did not significantly differ between untreated and SIT-treated groups. All these data suggested that decreased Type 2 CD4+ and CD8 + T cells might be closely correlated with the regulatory mechanisms of immunotherapy.

AB - Allergen-specific immunotherapy (IT) has been used for the treatment of atopic diseases since the turn of this century. The precise working mechanisms, however, remain to be clarified. The aim of this study was to investigate the role of particular subsets of allergen -specific T cells in the non-atopic individuals, untreated asthmatic children and the asthmatic children receiving immunotherapy. We collected peripheral blood from 16 untreated asthmatic children and 17 asthmatic children receiving immunotherapy over one and half years. All the patients were sensitive to mite allergen. Peripheral blood mononuclear cells (PBMC) were isolated and, in vitro, stimulated with crude mite extract to enrich the mite-specific T-cell population. After 14 days, the enriched mite-specific T cells were stimulated with phorbol-12-myristate-13-acetate (PMA) and ionomycin for intracellular detection of cytokines such as IFN-γ, IL-4 in CD4+ and CD8+ T lymphocytes. The data here demonstrated that the levels of mite-specific IgG4 and IgA increased significantly in asthmatic children after immunotherapy. In addition, both IL-4 expressing CD4+ and CD8+ T cells were significantly lower in asthmatic children after immunotherapy compared with those of before treatment and the normal control (p <0.05). In contrast, the frequency of IFN-γ expressing CD4+ and CD8+ T cells did not significantly differ between untreated and SIT-treated groups. All these data suggested that decreased Type 2 CD4+ and CD8 + T cells might be closely correlated with the regulatory mechanisms of immunotherapy.

KW - CD4 T cells

KW - CD8 T cells

KW - Immunotherapy

KW - Intracellular staining

UR - http://www.scopus.com/inward/record.url?scp=0042931073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042931073&partnerID=8YFLogxK

U2 - 10.1034/j.1399-3038.2003.00054.x

DO - 10.1034/j.1399-3038.2003.00054.x

M3 - Article

VL - 14

SP - 284

EP - 291

JO - Pediatric Allergy and Immunology

JF - Pediatric Allergy and Immunology

SN - 0905-6157

IS - 4

ER -