Bone regeneration using a porcine bone substitute collagen composite in vitro and in vivo

Eisner Salamanca, Chia Chen Hsu, Haw Ming Huang, Nai Chia Teng, Che Tong Lin, Yu Hwa Pan, Wei Jen Chang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The biocharacteristics of xenogeneic grafts make them a possible substitute for autogenous bone grafts in dental bone graft procedures. This study aimed to develop a novel porcine graft with collagen capable of generating new bone in bone defects via osteoconduction over 8 weeks of healing and to compare it with a porcine graft. The porcine collagen graft was made to undergo a cell viability test (MTT) and alkaline phosphatase assay (ALP). The surgical procedure was performed in 20 male adult New Zealand white rabbits. Four calvarial critical-size defects of 6 mm in diameter were prepared in each rabbit. The upper left defect was filled with a porcine graft of 500-1000 μm, the upper right with a porcine collagen graft, the lower left with hydroxyapatite/beta-tricalcium phosphate and the lower right served as the control without any filling material. The rabbits were divided and sacrificed at 2, 4, 6 and 8 weeks after surgery. Histological and micro-CT scan results showed that the performance of the porcine collagen graft is superior for regenerating new bone. Porcine collagen graft showed cell viability and osteoblast-like cell differentiation in vitro. The results indicate that porcine collagen graft is a potential bone substitute for clinical application.

Original languageEnglish
Article number984
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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Bone Substitutes
Bone Regeneration
Swine
Collagen
Transplants
Rabbits
Bone and Bones
Cell Survival
In Vitro Techniques
Osteoblasts
Alkaline Phosphatase
Cell Differentiation
Tooth

ASJC Scopus subject areas

  • General

Cite this

Bone regeneration using a porcine bone substitute collagen composite in vitro and in vivo. / Salamanca, Eisner; Hsu, Chia Chen; Huang, Haw Ming; Teng, Nai Chia; Lin, Che Tong; Pan, Yu Hwa; Chang, Wei Jen.

In: Scientific Reports, Vol. 8, No. 1, 984, 01.12.2018.

Research output: Contribution to journalArticle

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