Bone Marrow Combined With Dental Bud Cells Promotes Tooth Regeneration in Miniature Pig Model

Tzong Fu Kuo, Hsin Chi Lin, Kai Chiang Yang, Feng Huei Lin, Min Huey Chen, Chang Chin Wu, Hao Hueng Chang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Growth factors and morphogens secreted by bone marrow mesenchymal stem cells (BMSCs) of bone marrow fluid may promote tooth regeneration. Accordingly, a tissue engineering approach was utilized to develop an economical strategy for obtaining the growth factors and morphogens from BMSCs. Unerupted second molar tooth buds harvested from miniature pigs were cultured in vitro to obtain dental bud cells (DBCs). Bone marrow fluid, which contains BMSCs, was collected from the porcine mandible before operation. DBCs suspended in bone marrow fluid were seeded into a gelatin/chondoitin-6-sulfate/hyaluronan tri-copolymer scaffold (GCHT scaffold). The DBCs/bone marrow fluid/GCHT scaffold was autografted into the original alveolar sockets of the pigs. Radiographic and histological examinations were applied to identify the structure of regenerated tooth at 40 weeks postimplantation. The present results showed that one pig developed a complete tooth with crown, root, pulp, enamel, dentin, odontoblast, cementum, blood vessel, and periodontal ligament in indiscriminate shape. Three animals had an unerupted tooth that expressed dentin matrix protein-1, vascular endothelial growth factor, and osteopontin; and two other pigs also had dental-like structure with dentin tubules. This study reveals that DBCs adding bone marrow fluid and a suitable scaffold can promote the tooth regeneration in autogenic cell transplantation.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalArtificial Organs
Volume35
Issue number2
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

Fingerprint

Regeneration
Tooth
Bone
Swine
Bone Marrow
Stem cells
Fluids
Scaffolds
Dentin
Mesenchymal Stromal Cells
Hyaluronic Acid
Gelatin
Sulfates
Bone Marrow Cells
Intercellular Signaling Peptides and Proteins
Copolymers
Unerupted Tooth
Tooth Crown
Osteopontin
Odontoblasts

Keywords

  • Bone marrow fluid
  • Dental bud cell
  • Mesenchymal stem cell
  • Scaffold
  • Tooth regeneration

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

Bone Marrow Combined With Dental Bud Cells Promotes Tooth Regeneration in Miniature Pig Model. / Kuo, Tzong Fu; Lin, Hsin Chi; Yang, Kai Chiang; Lin, Feng Huei; Chen, Min Huey; Wu, Chang Chin; Chang, Hao Hueng.

In: Artificial Organs, Vol. 35, No. 2, 02.2011, p. 113-121.

Research output: Contribution to journalArticle

Kuo, Tzong Fu ; Lin, Hsin Chi ; Yang, Kai Chiang ; Lin, Feng Huei ; Chen, Min Huey ; Wu, Chang Chin ; Chang, Hao Hueng. / Bone Marrow Combined With Dental Bud Cells Promotes Tooth Regeneration in Miniature Pig Model. In: Artificial Organs. 2011 ; Vol. 35, No. 2. pp. 113-121.
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