Bone cell responses to a low elastic modulus titanium alloy surface immobilized with the natural cross-linker genipin

Ying Sui Sun, Chi Ya Huang, Chiang Sang Chen, Jean Heng Chang, Wen Tao Hou, Shu Jun Li, Yu Lin Hao, Haobo Pan, Her Hsiung Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)


In this study, we investigated various responses of human bone marrow mesenchymal stem cells (hMSCs) to a low elastic modulus Ti-24Nb-4Zr-8Sn (Ti2448) alloy surface that underwent immobilization using the natural cross-linker genipin. Specifically, hMSC's adhesion, proliferation, mineralization, and differentiation were evaluated. We also analyzed surface characteristics of the Ti2448 alloy, including chemistry, morphology, roughness, and wettability. Our results suggest that genipin immobilized Ti2448 has the potential to benefit orthopedic and dental implant applications. We found that the immobilization of genipin on a sand-blasted/acid-etched Ti2448 alloy surface significantly enhanced the mineralization of the extracellular matrix as well as the expression of certain osteogenic markers (bone sialoprotein and osteocalcin) in hMSCs. Conversely, surface roughness and hydrophilicity cannot be credited with the improved cellular responses observed in this study. The immobilization of genipin to produce a biomolecular surface on low elastic modulus Ti2448 alloy has considerable potential for bone implant applications.

Original languageEnglish
Pages (from-to)918-924
Number of pages7
JournalSurface and Coatings Technology
Publication statusPublished - Sep 25 2018



  • Cell response
  • Genipin
  • Immobilization
  • Low elastic modulus
  • Natural crosslinker
  • Ti alloy

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

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