Biosynthesis of d-aspartate in mammalian cells

Zhiqun Long, Hiroshi Homma, Jen Ai Lee, Takeshi Fukushima, Tomofumi Santa, Takeshi Iwatsubo, Ryo Hei Yamada, Kazuhiro Imai

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

In this communication, we demonstrate that d-aspartate (d-Asp) is synthesized in pheochromocytoma cells (PC12). To our knowledge this is the first report of biosynthesis of d-Asp in mammalian cells. Synthesis of d-Asp was demonstrated by its time-dependent accumulation in the cell culture, and by the fact that this accumulation was proportional to the number of inoculated cells. d-Asp in PC12 cells was identified by (i) co-elution with authentic d-Asp on two different HPLC columns, an octadesyl silica column and a Pirkle-type chiral column, (ii) reversed elution order of d-Asp and l-Asp on another Pirkle-type chiral column with an opposite configuration, and (iii) sensitivity to d-Asp oxidase. In the cells the amount of d-Asp was approx. 12-14% of total Asp and no other investigated d-amino acid was detected. The amount of d-Asp did not increase during the culture of mouse 3T3 fibroblasts and human neuroblastoma NB-1 cells. Immunocytochemical staining with anti-d-Asp antiserum demonstrated that d-Asp synthesized is present in the cytoplasm of the cells. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalFEBS Letters
Volume434
Issue number3
DOIs
Publication statusPublished - Sep 4 1998
Externally publishedYes

Fingerprint

Biosynthesis
Aspartic Acid
Cells
PC12 Cells
Cell culture
Pheochromocytoma
Fibroblasts
Neuroblastoma
Silicon Dioxide
Immune Sera
Oxidoreductases
Cytoplasm
Cell Culture Techniques
Cell Count
High Pressure Liquid Chromatography
Staining and Labeling
Amino Acids

Keywords

  • Biosynthesis
  • D-Amino acid
  • D-Aspartate
  • D-Serine
  • PC12

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Long, Z., Homma, H., Lee, J. A., Fukushima, T., Santa, T., Iwatsubo, T., ... Imai, K. (1998). Biosynthesis of d-aspartate in mammalian cells. FEBS Letters, 434(3), 231-235. https://doi.org/10.1016/S0014-5793(98)00986-7

Biosynthesis of d-aspartate in mammalian cells. / Long, Zhiqun; Homma, Hiroshi; Lee, Jen Ai; Fukushima, Takeshi; Santa, Tomofumi; Iwatsubo, Takeshi; Yamada, Ryo Hei; Imai, Kazuhiro.

In: FEBS Letters, Vol. 434, No. 3, 04.09.1998, p. 231-235.

Research output: Contribution to journalArticle

Long, Z, Homma, H, Lee, JA, Fukushima, T, Santa, T, Iwatsubo, T, Yamada, RH & Imai, K 1998, 'Biosynthesis of d-aspartate in mammalian cells', FEBS Letters, vol. 434, no. 3, pp. 231-235. https://doi.org/10.1016/S0014-5793(98)00986-7
Long Z, Homma H, Lee JA, Fukushima T, Santa T, Iwatsubo T et al. Biosynthesis of d-aspartate in mammalian cells. FEBS Letters. 1998 Sep 4;434(3):231-235. https://doi.org/10.1016/S0014-5793(98)00986-7
Long, Zhiqun ; Homma, Hiroshi ; Lee, Jen Ai ; Fukushima, Takeshi ; Santa, Tomofumi ; Iwatsubo, Takeshi ; Yamada, Ryo Hei ; Imai, Kazuhiro. / Biosynthesis of d-aspartate in mammalian cells. In: FEBS Letters. 1998 ; Vol. 434, No. 3. pp. 231-235.
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