Biomolecular clusters distribution up to mega dalton region using MALDI-quadrupole ion trap mass spectrometer

Yung Kun Chuang, Szu Hsueh Lai, Jung Lee Lin, Chung Hsuan Chen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r2 > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.

Original languageEnglish
Article number2789
JournalInternational Journal of Molecular Sciences
Volume19
Issue number9
DOIs
Publication statusPublished - Sep 17 2018

Keywords

  • Bovine serum albumin (BSA)
  • Cytochrome c
  • Large clusters
  • MALDI
  • Quantitative distribution

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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