Biomedical nanostructured coating for minimally invasive surgery devices applications: Characterization, cell cytotoxicity evaluation and an animal study in rat

Keng Liang Ou, Jan Show Chu, Hossein Hosseinkhani, Jeng Fong Chiou, Chih Hua Yu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Thermal injury and tissue sticking, which influence wound remodeling, are major concerns in electrosurgery. In this study, the effect of lateral thermal injury caused by different electrosurgical electrodes on hepatic remodeling was investigated. Methods: A monopolar electrosurgical unit equipped with untreated stainless steel (SS) and chromium nitride coated stainless steel (CrN-SS) electrodes was used to create lesions on the liver lobes of adult rats. Animals were sacrificed for evaluations at 0, 3, 7, and 28 days postoperatively. Results: CrN-SS needles generated lower levels of sticking tissue, and the thermographs showed that recorded highest temperature in liver tissue from the CrN-SS needle group was significantly lower than in the SS needle group. The total injury area of livers treated with CrN-SS needles was significantly lower than livers treated with SS needles at each time point. Moreover, the CrN-SS needles caused a relatively smaller area of lateral thermal injury, a smaller area of fibrotic tissue, and a faster process of hepatic remodeling in rat liver than the SS needles. Immunofluorescence staining and Western blot analysis showed that rats treated with CrN-SS needles expressed lower levels of NF-κB and caspase-3 postoperatively. Conclusions: This study reveals that the plating of electrodes with a CrN film is an efficient method for improving the performance of electrosurgical units and should benefit wound remodeling. However, more tests must be performed to confirm these promising findings in human patients.

Original languageEnglish
Pages (from-to)2174-2188
Number of pages15
JournalSurgical Endoscopy and Other Interventional Techniques
Volume28
Issue number7
DOIs
Publication statusPublished - 2014

Fingerprint

Minimally Invasive Surgical Procedures
Stainless Steel
Needles
Chromium
Equipment and Supplies
Liver
Wounds and Injuries
Electrodes
Hot Temperature
Electrosurgery
Caspase 3
Fluorescent Antibody Technique
Western Blotting
Staining and Labeling
Temperature

Keywords

  • Cytotoxicity
  • In vivo test
  • Minimally invasive surgery
  • Surface modification
  • Thermal injury
  • Tissue sticking

ASJC Scopus subject areas

  • Surgery

Cite this

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title = "Biomedical nanostructured coating for minimally invasive surgery devices applications: Characterization, cell cytotoxicity evaluation and an animal study in rat",
abstract = "Background: Thermal injury and tissue sticking, which influence wound remodeling, are major concerns in electrosurgery. In this study, the effect of lateral thermal injury caused by different electrosurgical electrodes on hepatic remodeling was investigated. Methods: A monopolar electrosurgical unit equipped with untreated stainless steel (SS) and chromium nitride coated stainless steel (CrN-SS) electrodes was used to create lesions on the liver lobes of adult rats. Animals were sacrificed for evaluations at 0, 3, 7, and 28 days postoperatively. Results: CrN-SS needles generated lower levels of sticking tissue, and the thermographs showed that recorded highest temperature in liver tissue from the CrN-SS needle group was significantly lower than in the SS needle group. The total injury area of livers treated with CrN-SS needles was significantly lower than livers treated with SS needles at each time point. Moreover, the CrN-SS needles caused a relatively smaller area of lateral thermal injury, a smaller area of fibrotic tissue, and a faster process of hepatic remodeling in rat liver than the SS needles. Immunofluorescence staining and Western blot analysis showed that rats treated with CrN-SS needles expressed lower levels of NF-κB and caspase-3 postoperatively. Conclusions: This study reveals that the plating of electrodes with a CrN film is an efficient method for improving the performance of electrosurgical units and should benefit wound remodeling. However, more tests must be performed to confirm these promising findings in human patients.",
keywords = "Cytotoxicity, In vivo test, Minimally invasive surgery, Surface modification, Thermal injury, Tissue sticking",
author = "Ou, {Keng Liang} and Chu, {Jan Show} and Hossein Hosseinkhani and Chiou, {Jeng Fong} and Yu, {Chih Hua}",
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T1 - Biomedical nanostructured coating for minimally invasive surgery devices applications

T2 - Characterization, cell cytotoxicity evaluation and an animal study in rat

AU - Ou, Keng Liang

AU - Chu, Jan Show

AU - Hosseinkhani, Hossein

AU - Chiou, Jeng Fong

AU - Yu, Chih Hua

PY - 2014

Y1 - 2014

N2 - Background: Thermal injury and tissue sticking, which influence wound remodeling, are major concerns in electrosurgery. In this study, the effect of lateral thermal injury caused by different electrosurgical electrodes on hepatic remodeling was investigated. Methods: A monopolar electrosurgical unit equipped with untreated stainless steel (SS) and chromium nitride coated stainless steel (CrN-SS) electrodes was used to create lesions on the liver lobes of adult rats. Animals were sacrificed for evaluations at 0, 3, 7, and 28 days postoperatively. Results: CrN-SS needles generated lower levels of sticking tissue, and the thermographs showed that recorded highest temperature in liver tissue from the CrN-SS needle group was significantly lower than in the SS needle group. The total injury area of livers treated with CrN-SS needles was significantly lower than livers treated with SS needles at each time point. Moreover, the CrN-SS needles caused a relatively smaller area of lateral thermal injury, a smaller area of fibrotic tissue, and a faster process of hepatic remodeling in rat liver than the SS needles. Immunofluorescence staining and Western blot analysis showed that rats treated with CrN-SS needles expressed lower levels of NF-κB and caspase-3 postoperatively. Conclusions: This study reveals that the plating of electrodes with a CrN film is an efficient method for improving the performance of electrosurgical units and should benefit wound remodeling. However, more tests must be performed to confirm these promising findings in human patients.

AB - Background: Thermal injury and tissue sticking, which influence wound remodeling, are major concerns in electrosurgery. In this study, the effect of lateral thermal injury caused by different electrosurgical electrodes on hepatic remodeling was investigated. Methods: A monopolar electrosurgical unit equipped with untreated stainless steel (SS) and chromium nitride coated stainless steel (CrN-SS) electrodes was used to create lesions on the liver lobes of adult rats. Animals were sacrificed for evaluations at 0, 3, 7, and 28 days postoperatively. Results: CrN-SS needles generated lower levels of sticking tissue, and the thermographs showed that recorded highest temperature in liver tissue from the CrN-SS needle group was significantly lower than in the SS needle group. The total injury area of livers treated with CrN-SS needles was significantly lower than livers treated with SS needles at each time point. Moreover, the CrN-SS needles caused a relatively smaller area of lateral thermal injury, a smaller area of fibrotic tissue, and a faster process of hepatic remodeling in rat liver than the SS needles. Immunofluorescence staining and Western blot analysis showed that rats treated with CrN-SS needles expressed lower levels of NF-κB and caspase-3 postoperatively. Conclusions: This study reveals that the plating of electrodes with a CrN film is an efficient method for improving the performance of electrosurgical units and should benefit wound remodeling. However, more tests must be performed to confirm these promising findings in human patients.

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KW - In vivo test

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KW - Surface modification

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