Biomarkers of oxidative stress associated with the risk of potentially malignant oral disorders

T. Senghore, Y.-F. Li, F.-C. Sung, M.-H. Tsai, C.-H. Hua, C.-S. Liu, R.-J. Huang, C.-C. Yeh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aim: To investigate the effect of oxidative stress biomarkers on the risk of potentially malignant oral disorders (PMODs). Materials and Methods: A total of 208 male adults with PMODs and an equal number of same-age control patients were enrolled. Plasma biomarkers of oxidative stress, measured with 8-hydroxy-2’-deoxyguanosine (8-OHdG) and 8-isoprostane (8-ISO), were determined using enzyme-linked immunosorbent assay (ELISA) kits. PMODs were diagnosed in accordance with the World Health Organization (WHO) guidelines. Results: A significant association between a high level of 8-ISO and an increased risk of PMODs was identified [odds ratio (OR)=1.71, 95% confidence interval (CI)=1.12-2.63; p=0.013]. This positive association was stronger among patients with PMOD subtype of leukoplakia (OR=1.94, 95% Cl=1.24-3.06; p=0.004). However, no significant association was observed between plasma 8-OHdG levels and overall risk of PMODs or subtypes. Conclusion: Increased plasma 8-ISO levels may indicate the prominence of lipid peroxidation in the development of PMODs, particularly leukoplakia. © 2018 International Institute of Anticancer Research. All rights reserved.
Original languageEnglish
Pages (from-to)5211-5216
Number of pages6
JournalAnticancer Research
Volume38
Issue number9
Publication statusPublished - 2018

Fingerprint

8-epi-prostaglandin F2alpha
Leukoplakia
Oxidative Stress
Biomarkers
Odds Ratio
Lipid Peroxidation
Enzyme-Linked Immunosorbent Assay
Guidelines
Confidence Intervals

Keywords

  • Biomarkers
  • Leukoplakia
  • Oxidative stress
  • Potentially malignant oral disorders
  • 8 hydroxydeoxyguanosine
  • 8 isoprostane
  • biological marker
  • genomic DNA
  • 8-epi-prostaglandin F2alpha
  • 8-oxo-7-hydrodeoxyguanosine
  • deoxyguanosine
  • prostaglandin F2 alpha
  • adult
  • alcohol consumption
  • areca nut
  • Article
  • cancer risk
  • case control study
  • cigarette smoking
  • controlled study
  • DNA isolation
  • enzyme linked immunosorbent assay
  • genotyping technique
  • human
  • leukoplakia
  • lipid peroxidation
  • major clinical study
  • male
  • malignant transformation
  • mastication
  • measurement
  • mouth cancer
  • oxidative stress
  • potentially malignant oral disorder
  • priority journal
  • protein blood level
  • risk assessment
  • risk factor
  • analogs and derivatives
  • blood
  • middle aged
  • mouth tumor
  • precancer
  • Adult
  • Deoxyguanosine
  • Dinoprost
  • Humans
  • Lipid Peroxidation
  • Male
  • Middle Aged
  • Mouth Neoplasms
  • Oxidative Stress
  • Precancerous Conditions
  • Risk Factors

Cite this

Senghore, T., Li, Y-F., Sung, F-C., Tsai, M-H., Hua, C-H., Liu, C-S., ... Yeh, C-C. (2018). Biomarkers of oxidative stress associated with the risk of potentially malignant oral disorders. Anticancer Research, 38(9), 5211-5216.

Biomarkers of oxidative stress associated with the risk of potentially malignant oral disorders. / Senghore, T.; Li, Y.-F.; Sung, F.-C.; Tsai, M.-H.; Hua, C.-H.; Liu, C.-S.; Huang, R.-J.; Yeh, C.-C.

In: Anticancer Research, Vol. 38, No. 9, 2018, p. 5211-5216.

Research output: Contribution to journalArticle

Senghore, T, Li, Y-F, Sung, F-C, Tsai, M-H, Hua, C-H, Liu, C-S, Huang, R-J & Yeh, C-C 2018, 'Biomarkers of oxidative stress associated with the risk of potentially malignant oral disorders', Anticancer Research, vol. 38, no. 9, pp. 5211-5216.
Senghore T, Li Y-F, Sung F-C, Tsai M-H, Hua C-H, Liu C-S et al. Biomarkers of oxidative stress associated with the risk of potentially malignant oral disorders. Anticancer Research. 2018;38(9):5211-5216.
Senghore, T. ; Li, Y.-F. ; Sung, F.-C. ; Tsai, M.-H. ; Hua, C.-H. ; Liu, C.-S. ; Huang, R.-J. ; Yeh, C.-C. / Biomarkers of oxidative stress associated with the risk of potentially malignant oral disorders. In: Anticancer Research. 2018 ; Vol. 38, No. 9. pp. 5211-5216.
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abstract = "Aim: To investigate the effect of oxidative stress biomarkers on the risk of potentially malignant oral disorders (PMODs). Materials and Methods: A total of 208 male adults with PMODs and an equal number of same-age control patients were enrolled. Plasma biomarkers of oxidative stress, measured with 8-hydroxy-2’-deoxyguanosine (8-OHdG) and 8-isoprostane (8-ISO), were determined using enzyme-linked immunosorbent assay (ELISA) kits. PMODs were diagnosed in accordance with the World Health Organization (WHO) guidelines. Results: A significant association between a high level of 8-ISO and an increased risk of PMODs was identified [odds ratio (OR)=1.71, 95{\%} confidence interval (CI)=1.12-2.63; p=0.013]. This positive association was stronger among patients with PMOD subtype of leukoplakia (OR=1.94, 95{\%} Cl=1.24-3.06; p=0.004). However, no significant association was observed between plasma 8-OHdG levels and overall risk of PMODs or subtypes. Conclusion: Increased plasma 8-ISO levels may indicate the prominence of lipid peroxidation in the development of PMODs, particularly leukoplakia. {\circledC} 2018 International Institute of Anticancer Research. All rights reserved.",
keywords = "Biomarkers, Leukoplakia, Oxidative stress, Potentially malignant oral disorders, 8 hydroxydeoxyguanosine, 8 isoprostane, biological marker, genomic DNA, 8-epi-prostaglandin F2alpha, 8-oxo-7-hydrodeoxyguanosine, deoxyguanosine, prostaglandin F2 alpha, adult, alcohol consumption, areca nut, Article, cancer risk, case control study, cigarette smoking, controlled study, DNA isolation, enzyme linked immunosorbent assay, genotyping technique, human, leukoplakia, lipid peroxidation, major clinical study, male, malignant transformation, mastication, measurement, mouth cancer, oxidative stress, potentially malignant oral disorder, priority journal, protein blood level, risk assessment, risk factor, analogs and derivatives, blood, middle aged, mouth tumor, precancer, Adult, Deoxyguanosine, Dinoprost, Humans, Lipid Peroxidation, Male, Middle Aged, Mouth Neoplasms, Oxidative Stress, Precancerous Conditions, Risk Factors, Biomarkers, Leukoplakia, Oxidative stress, Potentially malignant oral disorders",
author = "T. Senghore and Y.-F. Li and F.-C. Sung and M.-H. Tsai and C.-H. Hua and C.-S. Liu and R.-J. Huang and C.-C. Yeh",
note = "Export Date: 18 October 2018 CODEN: ANTRD 通訊地址: Yeh, C.-C.; School of Public Health, College of Public Health, Taipei Medical University, 250 Wu-Hsing Street, Taiwan; 電子郵件: ccyeh@tmu.edu.tw 化學物質/CAS: deoxyguanosine, 961-07-9; prostaglandin F2 alpha, 551-11-1; 8-epi-prostaglandin F2alpha; 8-oxo-7-hydrodeoxyguanosine; Biomarkers; Deoxyguanosine; Dinoprost 參考文獻: Su, C.-C., Tsai, K.-Y., Hsu, Y.-Y., Lin, Y.-Y., Lian, I.-B., Chronic exposure to heavy metals and risk of oral cancer in Taiwanese males (2010) Oral Oncol, 46 (8), pp. 586-590; Swenberg, J.A., Lu, K., Moeller, B.C., Gao, L., Upton, P.B., Nakamura, J., Starr, T.B., Endogenous versus exogenous DNA adducts: Their role in carcinogenesis, epidemiology, and risk assessment (2011) Toxicol Sci, 120, pp. S130-S145; Valko, M., Rhodes, C.J., Moncol, J., Izakovic, M., Mazur, M., Free radicals, metals and antioxidants in oxidative stress-induced cancer (2006) Chem Biol Interact, 160 (1), pp. 1-40; Marczynski, B., Rozynek, P., Kraus, T., Schlosser, S., Raithel, H.J., Baur, X., Levels of 8-hydroxy-2’-deoxyguanosine in DNA of white blood cells from workers highly exposed to asbestos in Germany (2000) Mutat Res, 468 (2), pp. 195-202; Xing, F., Li, Z., Sun, A., Xing, D., Reactive oxygen species promote chloroplast dysfunction and salicylic acid accumulation in fumonisin B1-induced cell death (2013) FEBS Lett, 587 (14), pp. 2164-2172; Thomson, C.A., Giuliano, A.R., Shaw, J.W., Rock, C.L., Ritenbaugh, C.K., Hakim, I.A., Hollenbach, K.A., Pierce, J.P., Diet and biomarkers of oxidative damage in women previously treated for breast cancer (2005) Nutr Cancer, 51 (2), pp. 146-154; Ma, N., Tagawa, T., Hiraku, Y., Murata, M., Ding, X., Kawanishi, S., 8-Nitroguanine formation in oral leukoplakia, a premalignant lesion (2006) Nitric Oxide, 14 (2), pp. 137-143; Choudhari, S.K., Chaudhary, M., Gadbail, A.R., Sharma, A., Tekade, S., Oxidative and antioxidative mechanisms in oral cancer and precancer: A review (2014) Oral Oncol, 50 (1), pp. 10-18; Sheweita, S.A., Drug-metabolizing enzymes: Mechanisms and functions (2000) Curr Drug Metab, 1 (2), pp. 107-132; Sosa, V., Molin{\'e}, T., Somoza, R., Paciucci, R., Kondoh, H., Lleonart, M.E., Oxidative stress and cancer: An overview (2013) Ageing Res Rev, 12 (1), pp. 376-390; Li, Y.F., Sung, F.C., Tsai, M.H., Hua, C.H., Liu, C.S., Huang, Y.T., Yeh, C.C., Interactions between cigarette smoking and polymorphisms of xenobiotic-metabolizing genes: The risk of oral leukoplakia (2013) Dis Markers, 34 (4), pp. 247-255; Kadashetti, V., Shivakumar, K.M., Chaudhary, M., Patil, S., Gawande, M., Hande, A., Influence of risk factors on patients suffering from potentially malignant disorders and oral cancer: A case–control study (2017) J Oral Maxillofac Pathol, 21 (3), pp. 455-456; Warnakulasuriya, S., Johnson, N.W., Van Der Waal, I., Nomenclature and classification of potentially malignant disorders of the oral mucosa (2007) J Oral Pathol Med, 36 (10), pp. 575-580; Speight, P.M., Khurram, S.A., Kujan, O., Oral potentially malignant disorders: Risk of progression to malignancy (2017) Oral Surg Oral Med Oral Pathol Oral Radiol, 125 (6), pp. 612-627; Chiang, C.-J., Lo, W.-C., Yang, Y.-W., You, S.-L., Chen, C.-J., Lai, M.S., Incidence and survival of adult cancer patients in Taiwan, 2002-2012 (2016) J Formos Med Assoc, 115 (12), pp. 1076-1088; Poulsen, H.E., Prieme, H., Loft, S., Role of oxidative DNA damage in cancer initiation and promotion (1998) Eur J Cancer Prev, 7 (1), pp. 9-16; Srivastava, K.C., Shrivastava, D., Analysis of plasma lipid peroxidation and antioxidant enzymes status in patients of oral leukoplakia: A case–control study (2016) J Int Soc Prev Community Dent, 6, pp. S213-s218; Ziech, D., Franco, R., Georgakilas, A.G., Georgakila, S., Malamou-Mitsi, V., Schoneveld, O., Pappa, A., Panayiotidis, M.I., The role of reactive oxygen species and oxidative stress in environmental carcinogenesis and biomarker development (2010) Chem Biol Interact, 188 (2), pp. 334-339; Kondo, S., Toyokuni, S., Tanaka, T., Hiai, H., Onodera, H., Kasai, H., Imamura, M., Overexpression of the hOGG1 gene and high 8-hydroxy-2’-deoxyguanosine (8-OHdG) lyase activity in human colorectal carcinoma: Regulation mechanism of the 8-OHdG level in DNA (2000) Clin Cancer Res, 6 (4), pp. 1394-1400; Niedernhofer, L.J., Daniels, J.S., Rouzer, C.A., Greene, R.E., Marnett, L.J., Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells (2003) J Biol Chem, 278 (33), pp. 31426-31433; Jr, R.P., Gammon, M.D., Terry, M.B., Agrawal, M., Zhang, F.F., Teitelbaum, S.L., Eng, S.M., Santella, R.M., Relationship between urinary 15-F2t-isoprostane and 8-oxodeoxyguanosine levels and breast cancer risk (2006) Cancer Epidemiol Biomarkers Prev, 15 (4), pp. 639-644; Brys, M., Morel, A., Forma, E., Krzeslak, A., Wilkosz, J., Rozanski, W., Olas, B., Relationship of urinary isoprostanes to prostate cancer occurrence (2013) Mol Cell Biochem, 372 (1-2), pp. 149-153; Freitas Bde, J., Lloret, G.R., Visacri, M.B., Tuan, B.T., Amaral, L.S., Baldini, D., De Sousa, V.M., Moriel, P., High 15-F2t-isoprostane levels in patients with a previous history of nonmelanoma skin cancer: The effects of supplementary antioxidant therapy (2015) Biomed Res Int, 2015, p. 963569; Benamira, M., Johnson, K., Chaudhary, A., Bruner, K., Tibbetts, C., Marnett, L.J., Induction of mutations by replication of malondialdehyde modified M13 DNA in Escherichia coli: Determination of the extent of DNA modification, genetic requirements for mutagenesis, and types of mutations induced (1995) Carcinogenesis, 16 (1), pp. 93-99; Liu, H., Jia, J., Mao, X., Lin, Z., Association of CYP1A1 and GSTM1 polymorphisms with oral cancer susceptibility: A meta-analysis (2015) Medicine, 94 (27), p. e895; Chole, R.H., Patil, R.N., Basak, A., Palandurkar, K., Bhowate, R., Estimation of serum malondialdehyde in oral cancer and precancer and its association with healthy individuals, gender, alcohol, and tobacco abuse (2010) J Cancer Res Ther, 6 (4), pp. 487-491; Valko, M., Jomova, K., Rhodes, C.J., Kuca, K., Musilek, K., Redox- And non-redox-metal-induced formation of free radicals and their role in human disease (2016) Arch Toxicol, 90 (1), pp. 1-37; He, P., Wei, M., Wang, Y., Liu, Q., Associations among glutathione S-transferase T1, M1, and P1 polymorphisms and the risk of oral leukoplakia (2016) Genet Test Mol Biomarkers, 20 (6), pp. 312-321",
year = "2018",
language = "English",
volume = "38",
pages = "5211--5216",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "9",

}

TY - JOUR

T1 - Biomarkers of oxidative stress associated with the risk of potentially malignant oral disorders

AU - Senghore, T.

AU - Li, Y.-F.

AU - Sung, F.-C.

AU - Tsai, M.-H.

AU - Hua, C.-H.

AU - Liu, C.-S.

AU - Huang, R.-J.

AU - Yeh, C.-C.

N1 - Export Date: 18 October 2018 CODEN: ANTRD 通訊地址: Yeh, C.-C.; School of Public Health, College of Public Health, Taipei Medical University, 250 Wu-Hsing Street, Taiwan; 電子郵件: ccyeh@tmu.edu.tw 化學物質/CAS: deoxyguanosine, 961-07-9; prostaglandin F2 alpha, 551-11-1; 8-epi-prostaglandin F2alpha; 8-oxo-7-hydrodeoxyguanosine; Biomarkers; Deoxyguanosine; Dinoprost 參考文獻: Su, C.-C., Tsai, K.-Y., Hsu, Y.-Y., Lin, Y.-Y., Lian, I.-B., Chronic exposure to heavy metals and risk of oral cancer in Taiwanese males (2010) Oral Oncol, 46 (8), pp. 586-590; Swenberg, J.A., Lu, K., Moeller, B.C., Gao, L., Upton, P.B., Nakamura, J., Starr, T.B., Endogenous versus exogenous DNA adducts: Their role in carcinogenesis, epidemiology, and risk assessment (2011) Toxicol Sci, 120, pp. S130-S145; Valko, M., Rhodes, C.J., Moncol, J., Izakovic, M., Mazur, M., Free radicals, metals and antioxidants in oxidative stress-induced cancer (2006) Chem Biol Interact, 160 (1), pp. 1-40; Marczynski, B., Rozynek, P., Kraus, T., Schlosser, S., Raithel, H.J., Baur, X., Levels of 8-hydroxy-2’-deoxyguanosine in DNA of white blood cells from workers highly exposed to asbestos in Germany (2000) Mutat Res, 468 (2), pp. 195-202; Xing, F., Li, Z., Sun, A., Xing, D., Reactive oxygen species promote chloroplast dysfunction and salicylic acid accumulation in fumonisin B1-induced cell death (2013) FEBS Lett, 587 (14), pp. 2164-2172; Thomson, C.A., Giuliano, A.R., Shaw, J.W., Rock, C.L., Ritenbaugh, C.K., Hakim, I.A., Hollenbach, K.A., Pierce, J.P., Diet and biomarkers of oxidative damage in women previously treated for breast cancer (2005) Nutr Cancer, 51 (2), pp. 146-154; Ma, N., Tagawa, T., Hiraku, Y., Murata, M., Ding, X., Kawanishi, S., 8-Nitroguanine formation in oral leukoplakia, a premalignant lesion (2006) Nitric Oxide, 14 (2), pp. 137-143; Choudhari, S.K., Chaudhary, M., Gadbail, A.R., Sharma, A., Tekade, S., Oxidative and antioxidative mechanisms in oral cancer and precancer: A review (2014) Oral Oncol, 50 (1), pp. 10-18; Sheweita, S.A., Drug-metabolizing enzymes: Mechanisms and functions (2000) Curr Drug Metab, 1 (2), pp. 107-132; Sosa, V., Moliné, T., Somoza, R., Paciucci, R., Kondoh, H., Lleonart, M.E., Oxidative stress and cancer: An overview (2013) Ageing Res Rev, 12 (1), pp. 376-390; Li, Y.F., Sung, F.C., Tsai, M.H., Hua, C.H., Liu, C.S., Huang, Y.T., Yeh, C.C., Interactions between cigarette smoking and polymorphisms of xenobiotic-metabolizing genes: The risk of oral leukoplakia (2013) Dis Markers, 34 (4), pp. 247-255; Kadashetti, V., Shivakumar, K.M., Chaudhary, M., Patil, S., Gawande, M., Hande, A., Influence of risk factors on patients suffering from potentially malignant disorders and oral cancer: A case–control study (2017) J Oral Maxillofac Pathol, 21 (3), pp. 455-456; Warnakulasuriya, S., Johnson, N.W., Van Der Waal, I., Nomenclature and classification of potentially malignant disorders of the oral mucosa (2007) J Oral Pathol Med, 36 (10), pp. 575-580; Speight, P.M., Khurram, S.A., Kujan, O., Oral potentially malignant disorders: Risk of progression to malignancy (2017) Oral Surg Oral Med Oral Pathol Oral Radiol, 125 (6), pp. 612-627; Chiang, C.-J., Lo, W.-C., Yang, Y.-W., You, S.-L., Chen, C.-J., Lai, M.S., Incidence and survival of adult cancer patients in Taiwan, 2002-2012 (2016) J Formos Med Assoc, 115 (12), pp. 1076-1088; Poulsen, H.E., Prieme, H., Loft, S., Role of oxidative DNA damage in cancer initiation and promotion (1998) Eur J Cancer Prev, 7 (1), pp. 9-16; Srivastava, K.C., Shrivastava, D., Analysis of plasma lipid peroxidation and antioxidant enzymes status in patients of oral leukoplakia: A case–control study (2016) J Int Soc Prev Community Dent, 6, pp. S213-s218; Ziech, D., Franco, R., Georgakilas, A.G., Georgakila, S., Malamou-Mitsi, V., Schoneveld, O., Pappa, A., Panayiotidis, M.I., The role of reactive oxygen species and oxidative stress in environmental carcinogenesis and biomarker development (2010) Chem Biol Interact, 188 (2), pp. 334-339; Kondo, S., Toyokuni, S., Tanaka, T., Hiai, H., Onodera, H., Kasai, H., Imamura, M., Overexpression of the hOGG1 gene and high 8-hydroxy-2’-deoxyguanosine (8-OHdG) lyase activity in human colorectal carcinoma: Regulation mechanism of the 8-OHdG level in DNA (2000) Clin Cancer Res, 6 (4), pp. 1394-1400; Niedernhofer, L.J., Daniels, J.S., Rouzer, C.A., Greene, R.E., Marnett, L.J., Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells (2003) J Biol Chem, 278 (33), pp. 31426-31433; Jr, R.P., Gammon, M.D., Terry, M.B., Agrawal, M., Zhang, F.F., Teitelbaum, S.L., Eng, S.M., Santella, R.M., Relationship between urinary 15-F2t-isoprostane and 8-oxodeoxyguanosine levels and breast cancer risk (2006) Cancer Epidemiol Biomarkers Prev, 15 (4), pp. 639-644; Brys, M., Morel, A., Forma, E., Krzeslak, A., Wilkosz, J., Rozanski, W., Olas, B., Relationship of urinary isoprostanes to prostate cancer occurrence (2013) Mol Cell Biochem, 372 (1-2), pp. 149-153; Freitas Bde, J., Lloret, G.R., Visacri, M.B., Tuan, B.T., Amaral, L.S., Baldini, D., De Sousa, V.M., Moriel, P., High 15-F2t-isoprostane levels in patients with a previous history of nonmelanoma skin cancer: The effects of supplementary antioxidant therapy (2015) Biomed Res Int, 2015, p. 963569; Benamira, M., Johnson, K., Chaudhary, A., Bruner, K., Tibbetts, C., Marnett, L.J., Induction of mutations by replication of malondialdehyde modified M13 DNA in Escherichia coli: Determination of the extent of DNA modification, genetic requirements for mutagenesis, and types of mutations induced (1995) Carcinogenesis, 16 (1), pp. 93-99; Liu, H., Jia, J., Mao, X., Lin, Z., Association of CYP1A1 and GSTM1 polymorphisms with oral cancer susceptibility: A meta-analysis (2015) Medicine, 94 (27), p. e895; Chole, R.H., Patil, R.N., Basak, A., Palandurkar, K., Bhowate, R., Estimation of serum malondialdehyde in oral cancer and precancer and its association with healthy individuals, gender, alcohol, and tobacco abuse (2010) J Cancer Res Ther, 6 (4), pp. 487-491; Valko, M., Jomova, K., Rhodes, C.J., Kuca, K., Musilek, K., Redox- And non-redox-metal-induced formation of free radicals and their role in human disease (2016) Arch Toxicol, 90 (1), pp. 1-37; He, P., Wei, M., Wang, Y., Liu, Q., Associations among glutathione S-transferase T1, M1, and P1 polymorphisms and the risk of oral leukoplakia (2016) Genet Test Mol Biomarkers, 20 (6), pp. 312-321

PY - 2018

Y1 - 2018

N2 - Aim: To investigate the effect of oxidative stress biomarkers on the risk of potentially malignant oral disorders (PMODs). Materials and Methods: A total of 208 male adults with PMODs and an equal number of same-age control patients were enrolled. Plasma biomarkers of oxidative stress, measured with 8-hydroxy-2’-deoxyguanosine (8-OHdG) and 8-isoprostane (8-ISO), were determined using enzyme-linked immunosorbent assay (ELISA) kits. PMODs were diagnosed in accordance with the World Health Organization (WHO) guidelines. Results: A significant association between a high level of 8-ISO and an increased risk of PMODs was identified [odds ratio (OR)=1.71, 95% confidence interval (CI)=1.12-2.63; p=0.013]. This positive association was stronger among patients with PMOD subtype of leukoplakia (OR=1.94, 95% Cl=1.24-3.06; p=0.004). However, no significant association was observed between plasma 8-OHdG levels and overall risk of PMODs or subtypes. Conclusion: Increased plasma 8-ISO levels may indicate the prominence of lipid peroxidation in the development of PMODs, particularly leukoplakia. © 2018 International Institute of Anticancer Research. All rights reserved.

AB - Aim: To investigate the effect of oxidative stress biomarkers on the risk of potentially malignant oral disorders (PMODs). Materials and Methods: A total of 208 male adults with PMODs and an equal number of same-age control patients were enrolled. Plasma biomarkers of oxidative stress, measured with 8-hydroxy-2’-deoxyguanosine (8-OHdG) and 8-isoprostane (8-ISO), were determined using enzyme-linked immunosorbent assay (ELISA) kits. PMODs were diagnosed in accordance with the World Health Organization (WHO) guidelines. Results: A significant association between a high level of 8-ISO and an increased risk of PMODs was identified [odds ratio (OR)=1.71, 95% confidence interval (CI)=1.12-2.63; p=0.013]. This positive association was stronger among patients with PMOD subtype of leukoplakia (OR=1.94, 95% Cl=1.24-3.06; p=0.004). However, no significant association was observed between plasma 8-OHdG levels and overall risk of PMODs or subtypes. Conclusion: Increased plasma 8-ISO levels may indicate the prominence of lipid peroxidation in the development of PMODs, particularly leukoplakia. © 2018 International Institute of Anticancer Research. All rights reserved.

KW - Biomarkers

KW - Leukoplakia

KW - Oxidative stress

KW - Potentially malignant oral disorders

KW - 8 hydroxydeoxyguanosine

KW - 8 isoprostane

KW - biological marker

KW - genomic DNA

KW - 8-epi-prostaglandin F2alpha

KW - 8-oxo-7-hydrodeoxyguanosine

KW - deoxyguanosine

KW - prostaglandin F2 alpha

KW - adult

KW - alcohol consumption

KW - areca nut

KW - Article

KW - cancer risk

KW - case control study

KW - cigarette smoking

KW - controlled study

KW - DNA isolation

KW - enzyme linked immunosorbent assay

KW - genotyping technique

KW - human

KW - leukoplakia

KW - lipid peroxidation

KW - major clinical study

KW - male

KW - malignant transformation

KW - mastication

KW - measurement

KW - mouth cancer

KW - oxidative stress

KW - potentially malignant oral disorder

KW - priority journal

KW - protein blood level

KW - risk assessment

KW - risk factor

KW - analogs and derivatives

KW - blood

KW - middle aged

KW - mouth tumor

KW - precancer

KW - Adult

KW - Deoxyguanosine

KW - Dinoprost

KW - Humans

KW - Lipid Peroxidation

KW - Male

KW - Middle Aged

KW - Mouth Neoplasms

KW - Oxidative Stress

KW - Precancerous Conditions

KW - Risk Factors

KW - Biomarkers

KW - Leukoplakia

KW - Oxidative stress

KW - Potentially malignant oral disorders

M3 - Article

VL - 38

SP - 5211

EP - 5216

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 9

ER -