Biomarkers of DNA damage in patients with end-stage renal disease: Mitochondrial DNA mutation in hair follicles

Chin San Liu, Lie Yon Ko, Paik Seong Lim, Shu Huei Kao, Yau Huei Wei

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background. DNA damage was noted in patients with end-stage renal disease (ESRD). Mitochondrial DNA (mtDNA) mutations have been proposed as a genomic biomarker in the process of human ageing, degenerative diseases and carcinogenesis. Methods. Polymerase chain reaction (PCR) techniques were applied to detect mtDNA deletions in hair follicles, an appendage of skin, from 162 patients with ESRD. Results. The incidences of the 4977 bp deletion of mtDNA in hair follicles were found to increase with age in normal control and ESRD patients. As compared with normal subjects, ESRD patients had 3.5, 2.3, 2.7, 2.3 and 1.4 times higher incidences of the 4977 bp deletion of mtDNA in the age groups of 20-30, 31-40, 41-50, 51-60 and 61-70 years, respectively. Moreover, the difference in the proportion of mtDNA with the 4977 bp deletion was statistically significant between ESRD patients and normal subjects >50 years of age. Conclusion. We suggest that the 4977 bp deletion of mtDNA in hair follicles may serve as one of the tissue biomarkers of genetic instability of the mitochondrial genome in ESRD patients.

Original languageEnglish
Pages (from-to)561-565
Number of pages5
JournalNephrology Dialysis Transplantation
Volume16
Issue number3
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Hair Follicle
Mitochondrial DNA
DNA Damage
Chronic Kidney Failure
Biomarkers
Mutation
Mitochondrial Genome
Incidence
Carcinogenesis
Age Groups
Polymerase Chain Reaction
Skin

Keywords

  • Biomarker
  • Deletion
  • End-stage renal disease
  • Hair follicles
  • Mitochondrial DNA

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Biomarkers of DNA damage in patients with end-stage renal disease : Mitochondrial DNA mutation in hair follicles. / Liu, Chin San; Ko, Lie Yon; Lim, Paik Seong; Kao, Shu Huei; Wei, Yau Huei.

In: Nephrology Dialysis Transplantation, Vol. 16, No. 3, 2001, p. 561-565.

Research output: Contribution to journalArticle

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AU - Wei, Yau Huei

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