Abstract

To improve the biological activity of titanium, by using of glow discharge plasma (GDP), albumin-grafted titanium disk have been implemented and carefully studied. Titanium disks were pre-treated with GDP in an environment filled with argon and allylamine gas. Glutaraldehyde was used as a cross-linking agent for albumin grafting. Then, the surface of the albumin-grafted titanium was examined using scanning electron microscopy and X-ray photoelectron spectroscopy. In addition, the static water contact angles of the albumin-grafted titanium disks were measured using goniometry. To observe the effects of albumin adsorption on cell behavior, MG-63 osteoblast-like cells were cultured on the surface-modified titanium disks. Blood coagulation resistance of the modified titanium was monitored and compared to the control titanium disks. The results demonstrated that MG-63 osteoblast-like cells cultured on the albumin-grafted titanium disks expressed better-differentiated morphology compare to cells grown on the control disks. Furthermore, albumin-grafting treatment significantly improved the surface wettability of the titanium disks and resulted in a significantly negative effect on thrombus formation. Based on these results, it was believed that the GDP can potentially improve the biofunctionality of titanium surfaces.

Original languageEnglish
Pages (from-to)701-706
Number of pages6
JournalMedical and Biological Engineering and Computing
Volume49
Issue number6
DOIs
Publication statusPublished - Jun 2011

Fingerprint

Glow discharges
Titanium
Surface treatment
Plasmas
Albumins
Osteoblasts
Cultured Cells
Allylamine
Wettability
Photoelectron Spectroscopy
Argon
Glutaral
Blood Coagulation
Bioactivity
Coagulation
Serum Albumin
Electron Scanning Microscopy
Adsorption
Contact angle
Wetting

Keywords

  • Albumin
  • Glow discharge
  • Surface modification
  • Tissue engineering
  • Titanium

ASJC Scopus subject areas

  • Biomedical Engineering
  • Computer Science Applications
  • Medicine(all)

Cite this

@article{d93561a966f34a93a0175aafbb3c4330,
title = "Biological surface modification of titanium surfaces using glow discharge plasma",
abstract = "To improve the biological activity of titanium, by using of glow discharge plasma (GDP), albumin-grafted titanium disk have been implemented and carefully studied. Titanium disks were pre-treated with GDP in an environment filled with argon and allylamine gas. Glutaraldehyde was used as a cross-linking agent for albumin grafting. Then, the surface of the albumin-grafted titanium was examined using scanning electron microscopy and X-ray photoelectron spectroscopy. In addition, the static water contact angles of the albumin-grafted titanium disks were measured using goniometry. To observe the effects of albumin adsorption on cell behavior, MG-63 osteoblast-like cells were cultured on the surface-modified titanium disks. Blood coagulation resistance of the modified titanium was monitored and compared to the control titanium disks. The results demonstrated that MG-63 osteoblast-like cells cultured on the albumin-grafted titanium disks expressed better-differentiated morphology compare to cells grown on the control disks. Furthermore, albumin-grafting treatment significantly improved the surface wettability of the titanium disks and resulted in a significantly negative effect on thrombus formation. Based on these results, it was believed that the GDP can potentially improve the biofunctionality of titanium surfaces.",
keywords = "Albumin, Glow discharge, Surface modification, Tissue engineering, Titanium",
author = "Huang, {Haw Ming} and Hsieh, {Sung Chih} and Teng, {Nai Chia} and Sheng-Wei Feng and Ou, {Ken Liang} and Chang, {Wei Jen}",
year = "2011",
month = "6",
doi = "10.1007/s11517-011-0742-2",
language = "English",
volume = "49",
pages = "701--706",
journal = "Medical and Biological Engineering and Computing",
issn = "0140-0118",
publisher = "Peter Peregrinus Ltd",
number = "6",

}

TY - JOUR

T1 - Biological surface modification of titanium surfaces using glow discharge plasma

AU - Huang, Haw Ming

AU - Hsieh, Sung Chih

AU - Teng, Nai Chia

AU - Feng, Sheng-Wei

AU - Ou, Ken Liang

AU - Chang, Wei Jen

PY - 2011/6

Y1 - 2011/6

N2 - To improve the biological activity of titanium, by using of glow discharge plasma (GDP), albumin-grafted titanium disk have been implemented and carefully studied. Titanium disks were pre-treated with GDP in an environment filled with argon and allylamine gas. Glutaraldehyde was used as a cross-linking agent for albumin grafting. Then, the surface of the albumin-grafted titanium was examined using scanning electron microscopy and X-ray photoelectron spectroscopy. In addition, the static water contact angles of the albumin-grafted titanium disks were measured using goniometry. To observe the effects of albumin adsorption on cell behavior, MG-63 osteoblast-like cells were cultured on the surface-modified titanium disks. Blood coagulation resistance of the modified titanium was monitored and compared to the control titanium disks. The results demonstrated that MG-63 osteoblast-like cells cultured on the albumin-grafted titanium disks expressed better-differentiated morphology compare to cells grown on the control disks. Furthermore, albumin-grafting treatment significantly improved the surface wettability of the titanium disks and resulted in a significantly negative effect on thrombus formation. Based on these results, it was believed that the GDP can potentially improve the biofunctionality of titanium surfaces.

AB - To improve the biological activity of titanium, by using of glow discharge plasma (GDP), albumin-grafted titanium disk have been implemented and carefully studied. Titanium disks were pre-treated with GDP in an environment filled with argon and allylamine gas. Glutaraldehyde was used as a cross-linking agent for albumin grafting. Then, the surface of the albumin-grafted titanium was examined using scanning electron microscopy and X-ray photoelectron spectroscopy. In addition, the static water contact angles of the albumin-grafted titanium disks were measured using goniometry. To observe the effects of albumin adsorption on cell behavior, MG-63 osteoblast-like cells were cultured on the surface-modified titanium disks. Blood coagulation resistance of the modified titanium was monitored and compared to the control titanium disks. The results demonstrated that MG-63 osteoblast-like cells cultured on the albumin-grafted titanium disks expressed better-differentiated morphology compare to cells grown on the control disks. Furthermore, albumin-grafting treatment significantly improved the surface wettability of the titanium disks and resulted in a significantly negative effect on thrombus formation. Based on these results, it was believed that the GDP can potentially improve the biofunctionality of titanium surfaces.

KW - Albumin

KW - Glow discharge

KW - Surface modification

KW - Tissue engineering

KW - Titanium

UR - http://www.scopus.com/inward/record.url?scp=79959886846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959886846&partnerID=8YFLogxK

U2 - 10.1007/s11517-011-0742-2

DO - 10.1007/s11517-011-0742-2

M3 - Article

VL - 49

SP - 701

EP - 706

JO - Medical and Biological Engineering and Computing

JF - Medical and Biological Engineering and Computing

SN - 0140-0118

IS - 6

ER -